Enhancing the radiotherapeutic index of gamma radiation on cervical cancer cells by gold nanoparticles

Yadav, Priya; Bandyopadhyay, A. K.; Chakraborty, Anindita; Sarkar, Keka

doi:10.1007/s13404-019-00260-2

Cited by 8 publications

(8 citation statements)

References 44 publications

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“…The diffusion cell experiments performed with the porcine vaginal mucosa membrane using PET imaging revealed the kinetics of AuNP release from the hydrogel, their accumulation in the membrane, and permeation across it and into the AC. PET is ∼1000 times more sensitive than spectroscopic analytical techniques usually employed for diffusion cell measurements, and it allows a continuous acquisition of a signal for a period of several days. , Overall, the diffusion cell results demonstrated that a very large fraction (47%) of AuNPs accumulated in the membrane after 42 h. This is a very significant result since potential toxicity of AuNPs to the uterus and other healthy tissues should be considered at all steps in the development of future cervical cancer therapies. − The gradual liberation of AuNPs from the hydrogel, coupled to their very strong retention at the membrane site, is in agreement with the requirements of AuNP administration locally prior to photothermal and radiosensitization procedures.…”

Section: Discussionmentioning

confidence: 89%

“…Photothermal and brachytherapy applications are being developed for the treatment of cervical cancer that often require the topical administration of therapeutic nanoparticles. − In the PTT method, the absorption of light in the visible and near-infrared region by AuNPs leads to local heat generation, which induces damage to cancer cells . In radiotherapy, AuNPs could be used as additives to enhance the effects of radiation on cancer tissues. ,, Moreover, in combination with chemotherapeutics ( e.g. , doxorubicin), they represent an improved approach for cancer chemoradiotherapy …”

Section: Discussionmentioning

confidence: 99%

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A Three-Dimensional Printable Hydrogel Formulation for the Local Delivery of Therapeutic Nanoparticles to Cervical Cancer

Kiseleva

Omar

Boisselier

et al. 2022

ACS Biomater. Sci. Eng.

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Cervical cancer is the fourth most common malignancy among women. Compared to other types of cancer, therapeutic agents can be administrated locally at the mucosal vaginal membrane. Thermosensitive gels have been developed over the years for contraception or for the treatment of bacterial, fungal, and sexually transmitted infections. These formulations often carry therapeutic nanoparticles and are now being considered in the arsenal of tools for oncology. They can also be three-dimensionally (3D) printed for a better geometrical adjustment to the anatomy of the patient, thus enhancing the local delivery treatment. In this study, a localized delivery system composed of a Pluronic F127-alginate hydrogel with efficient nanoparticle (NP) release properties was prepared for intravaginal application procedures. The kinetics of hydrogel degradation and its NP releasing properties were demonstrated with ultrasmall gold nanoparticles (∼80% of encapsulated AuNPs released in 48 h). The mucoadhesive properties of the hydrogel formulation were assayed by the periodic acid/Schiff reagent staining, which revealed that 19% of mucins were adsorbed on the gel’s surface. The hydrogel formulation was tested for cytocompatibility in three cell lines (HeLa, CRL 2616, and BT-474; no sign of cytotoxicity revealed). The release of AuNPs from the hydrogel and their accumulation in vaginal membranes were quantitatively measured in vitro/ex vivo with positron emission tomography, a highly sensitive modality allowing real-time imaging of nanoparticle diffusion (lag time to start of permeation of 3.3 h, 47% of AuNPs accumulated in the mucosa after 42 h). Finally, the potential of the AuNP-containing Pluronic F127-alginate hydrogel for 3D printing was demonstrated, and the geometrical precision of the 3D printed systems was measured by magnetic resonance imaging (<0.5 mm precision; deviation from the design values <2.5%). In summary, this study demonstrates the potential of Pluronic F127-alginate formulations for the topical administration of NP-releasing gels applied to vaginal wall therapy. This technology could open new possibilities for photothermal and radiosensitizing oncology applications.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

A Three-Dimensional Printable Hydrogel Formulation for the Local Delivery of Therapeutic Nanoparticles to Cervical Cancer

Kiseleva

Omar

Boisselier

et al. 2022

ACS Biomater. Sci. Eng.

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“…However, large shadow areas and necrotic-shaped cells were observed in Gd 2 O 3 @BSA-Au + X-ray groups, which confirm the effective destruction of tumor cells . Here, the presence of Au NPs could further enhance ROS levels and kill tumors by blocking cell proliferation …”

Section: Resultsmentioning

confidence: 86%

“… 10 Here, the presence of Au NPs could further enhance ROS levels and kill tumors by blocking cell proliferation. 45 …”

Section: Resultsmentioning

confidence: 99%

Enhanced In Vivo Radiotherapy of Breast Cancer Using Gadolinium Oxide and Gold Hybrid Nanoparticles

Nosrati

Salehiabar

Charmi

et al. 2023

ACS Appl. Bio Mater.

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Radiation therapy has demonstrated promising effectiveness against several types of cancers. X-ray radiation therapy can be made further effective by utilizing nanoparticles of high-atomic-number (high-Z) materials that act as radiosensitizers. Here, in purpose of maximizing the radiation therapy within tumors, bovine serum albumin capped gadolinium oxide and gold nanoparticles (Gd 2 O 3 @BSA-Au NPs) are developed as a bimetallic radiosensitizer. In this study, we incorporate two high-Z-based nanoparticles, Au and Gd, in a single nanoplatform. The radiosensitizing ability of the nanoparticles was assessed with a series of in vitro tests, following evaluation in vivo in a breast cancer murine model. Enhanced tumor suppression is observed in the group that received radiation after administration of Gd 2 O 3 @BSA-Au NPs. As a result, cancer therapy efficacy is significantly improved by applying Gd 2 O 3 @BSA-Au NPs under X-ray irradiation, as evidenced by studies evaluating cell viability, proliferation, reactive oxygen species production, and in vivo anti-tumor effect.

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“…Nanomaterials based on gold (Au) (Z = 79) are markedly attractive in studies on radiosensitizers, which is attributed to beneficial characteristics, including a simplified synthesis process, controllable size and shape, good biocompatibility and ease of surface modification [55,56]. The detailed mechanism of the tumor-killing effect of Au nanoparticles (NPs) under γ-radiation was first discovered by Yadav et al, who found that elevated ROS levels induced by Au NPs efficiently kill tumors by blocking cell proliferation and inducing apoptosis [41]. In further research, the efficiency of ROS generation induced by Au was found to be related to the size and shape of Au materials.…”

Section: Nanomaterials Enhance the Rt Effect Via Ros Generationmentioning

confidence: 99%

Promoting Reactive Oxygen Species Generation: A Key Strategy in Nanosensitizer-Mediated Radiotherapy

Jia

Fan

et al. 2021

Nanomedicine (Lond.)

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The radiotherapy enhancement effect of numerous nanosensitizers is based on the excessive production of reactive oxygen species (ROS), and only a few systematic reviews have focused on the key strategy in nanosensitizer-mediated radiotherapy. To clarify the mechanism underlying this effect, it is necessary to understand the role of ROS in radiosensitization before clinical application. Thus, the source of ROS and their principle of tumor inhibition are first introduced. Then, nanomaterial-mediated ROS generation in radiotherapy is reviewed. The double-edged sword effect of ROS and the potential dangers they may pose to cancer patients are subsequently addressed. Finally, future perspectives regarding ROS-regulated nanosensitizer applications and development are discussed.

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Enhancing the radiotherapeutic index of gamma radiation on cervical cancer cells by gold nanoparticles

Cited by 8 publications

References 44 publications

A Three-Dimensional Printable Hydrogel Formulation for the Local Delivery of Therapeutic Nanoparticles to Cervical Cancer

A Three-Dimensional Printable Hydrogel Formulation for the Local Delivery of Therapeutic Nanoparticles to Cervical Cancer

Enhanced In Vivo Radiotherapy of Breast Cancer Using Gadolinium Oxide and Gold Hybrid Nanoparticles

Promoting Reactive Oxygen Species Generation: A Key Strategy in Nanosensitizer-Mediated Radiotherapy

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