Enhancing the sensitivity of DNA microarrays by metal-enhanced fluorescence using vertical nanorod structures

Badshah, Mohsin Ali; Ju, Jonghyun; Lu, Xun; Abbas, Naseem; Kim, Seok-Min

doi:10.1016/j.snb.2018.07.163

Cited by 27 publications

(34 citation statements)

References 38 publications

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“…The total enhancement factor was ~210× and the additional ~36× enhancement might be due to the surface area increment and enhanced binding ability. These measured values using the protein were similar to the enhancement data using deoxyribonucleic acid (DNA) sample in previous research [ 31 ].…”

Section: Fabrication Of the Glad Mef Substratesupporting

confidence: 85%

“…The vertical Ag nanorods (500 nm) were formed on the substrate in the electron beam evaporation system under a vacuum condition of ~9.5 × 10 −7 Torr at a deposition rate of ~5 Å/s. The length of the Ag nanorod was selected for maximizing the fluorescence enhancement factor based on our previous study [ 31 ]. Figure 2 a,b show the top view and cross-sectional view, respectively, of the scanning electron microscopy (SEM) images of the fabricated MEF Ag nanorod substrate.…”

Section: Fabrication Of the Glad Mef Substratementioning

confidence: 99%

“…The mechanisms for the enhancement of fluorescence on vertical Ag nanorods MEF substrate can be categorized by the enhanced near-field, the increment in surface area, and the enhanced binding ability [ 31 ]. The most common reason for fluorescence enhancement on metallic nanostructure is the enhanced electromagnetic field generated by localized surface Plasmon resonance (LSPR).…”

Section: Fabrication Of the Glad Mef Substratementioning

confidence: 99%

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Development of a Protein Microarray Chip with Enhanced Fluorescence for Identification of Semen and Vaginal Fluid

Abbas

Badshah

et al. 2018

Sensors

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The detection of body fluids has been used to identify a suspect and build a criminal case. As the amount of evidence collected at a crime site is limited, a multiplex identification system for body fluids using a small amount of sample is required. In this study, we proposed a multiplex detection platform using an Ag vertical nanorod metal enhanced fluorescence (MEF) substrate for semen and vaginal fluid (VF), which are important evidence in cases of sexual crime. The Ag nanorod MEF substrate with a length of 500 nm was fabricated by glancing angle deposition, and amino functionalization was conducted to improve binding ability. The effect of incubation time was analyzed, and an incubation time of 60 min was selected, at which the fluorescence signal was saturated. To assess the performance of the developed identification chip, the identification of semen and VF was carried out. The developed sensor could selectively identify semen and VF without any cross-reactivity. The limit of detection of the fabricated microarray chip was 10 times better than the commercially available rapid stain identification (RSID) Semen kit.

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Section: Fabrication Of the Glad Mef Substratesupporting

confidence: 85%

Section: Fabrication Of the Glad Mef Substratementioning

confidence: 99%

Section: Fabrication Of the Glad Mef Substratementioning

confidence: 99%

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Development of a Protein Microarray Chip with Enhanced Fluorescence for Identification of Semen and Vaginal Fluid

Abbas

Badshah

et al. 2018

Sensors

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“…The size of the nanostructures governs the scattering and absorption ratios, active surface plasmon (SP) mode, the peak position of the plasmon mode, and localization of the plasmons [21]. Previous investigations [22] showed that the variations in size of the nanostructures can boost the MEF enhancement factor (MEF-EF). MEF-EF is defined as the ratio of fluorescence intensity between nanostructured and conventional glass substrates, measured at the same wavelength and under the same experimental conditions.…”

Section: Introductionmentioning

confidence: 99%

“…The shape of the nanostructures is another critical parameter for describing the plasmonic characteristics [20]. Different shapes of nanostructures such as nanowires [10], spheres [13], rods [19,22,23], cubes [24,25], triangles [26,27], and crescent-like structures [28], were developed with multiple methods to tune the spectral properties, i.e., to boost the efficiency and MEF-EF [9,29,30].…”

Section: Introductionmentioning

confidence: 99%

Recent Developments in Plasmonic Nanostructures for Metal Enhanced Fluorescence-Based Biosensing

Koh²,

et al. 2020

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Metal-enhanced fluorescence (MEF) is a unique phenomenon of surface plasmons, where light interacts with the metallic nanostructures and produces electromagnetic fields to enhance the sensitivity of fluorescence-based detection. In particular, this enhancement in sensing capacity is of importance to many research areas, including medical diagnostics, forensic science, and biotechnology. The article covers the basic mechanism of MEF and recent developments in plasmonic nanostructures fabrication for efficient fluorescence signal enhancement that are critically reviewed. The implications of current fluorescence-based technologies for biosensors are summarized, which are in practice to detect different analytes relevant to food control, medical diagnostics, and forensic science. Furthermore, characteristics of existing fabrication methods have been compared on the basis of their resolution, design flexibility, and throughput. The future projections emphasize exploring the potential of non-conventional materials and hybrid fabrication techniques to further enhance the sensitivity of MEF-based biosensors.

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Plasmonic Fluorescence Enhancement in Diagnostics for Clinical Tests at Point‐of‐Care: A Review of Recent Technologies

et al. 2022

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Fluorescence‐based biosensors have widely been used in the life‐sciences and biomedical applications due to their low limit of detection and a diverse selection of fluorophores that enable simultaneous measurements of multiple biomarkers. Recent research effort has been made to implement fluorescent biosensors into the exploding field of point‐of‐care testing (POCT), which uses cost‐effective strategies for rapid and affordable diagnostic testing. However, fluorescence‐based assays often suffer from their feeble signal at low analyte concentrations, which often requires sophisticated, costly, and bulky instrumentation to maintain high detection sensitivity. Metal‐ and metal oxide‐based nanostructures offer a simple solution to increase the output signal from fluorescent biosensors due to the generation of high field enhancements close to a metal or metal oxide surface, which has been shown to improve the excitation rate, quantum yield, photostability, and radiation pattern of fluorophores. This article provides an overview of existing biosensors that employ various strategies for fluorescence enhancement via nanostructures and have demonstrated the potential for use as POCT. Biosensors using nanostructures such as planar substrates, freestanding nanoparticles, and metal–dielectric–metal nanocavities are discussed with an emphasis placed on technologies that have shown promise towards POCT applications without the need for centralized laboratories.

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Enhancing the sensitivity of DNA microarrays by metal-enhanced fluorescence using vertical nanorod structures

Cited by 27 publications

References 38 publications

Development of a Protein Microarray Chip with Enhanced Fluorescence for Identification of Semen and Vaginal Fluid

Development of a Protein Microarray Chip with Enhanced Fluorescence for Identification of Semen and Vaginal Fluid

Recent Developments in Plasmonic Nanostructures for Metal Enhanced Fluorescence-Based Biosensing

Plasmonic Fluorescence Enhancement in Diagnostics for Clinical Tests at Point‐of‐Care: A Review of Recent Technologies

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