Enhancing xylanase expression by Komagataella phaffii by formate as carbon source and inducer

Liu, Bing; Li, Haijian; Zhou, Huan; Zhang, Jianguo

doi:10.1007/s00253-022-12249-7

Cited by 4 publications

(2 citation statements)

References 39 publications

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“…S1). Studies have demonstrated the efficacy of formate as both an inducer and a carbon source to produce recombinant proteins (rProt) in P. pastoris (Singh and Narang, 2020; Liu, Li, et al ., 2022; Liu, Zhao, et al ., 2022). Herein, an enhanced green fluorescent protein (eGFP) reporter system was used to probe the regulation of the AOX1 gene promoter (p AOX1 ) by formate.…”

Section: Resultsmentioning

confidence: 99%

Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficientPichia pastoris

Bustos,

Berrios,

Fickers

2024

Preprint

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InPichia pastoris(Komagataella phaffii), formate is a recognized alternative inducer to methanol for expression systems based on the AOX1 promoter (pAOX1). By disrupting the formate dehydrogenase encodingFDH1gene, we converted such a system into a self-induced one, as adding any inducer in the culture medium is no longer requested for pAOX1 induction. In cells, formate is generated from serine through the THF-C1 metabolism, and it cannot be converted into carbon dioxide in anfdh1Δ strain. Under non-repressive culture conditions, such as on sorbitol, the intracellular formate generated from the THF-C1 metabolism is sufficient to induce pAOX1 and initiate protein synthesis. This was evidenced for two model proteins, namely intracellular eGFP and secreted CalB lipase fromC. antarctica. Similar protein productivities were obtained for anfdh1Δ strain on sorbitol and a non-disrupted strain on sorbitol-methanol. Considering aP. pastoris fdh1Δstrain as a workhorse for recombinant protein synthesis paves the way for the further development of methanol-free processes inP. pastoris.

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Section: Resultsmentioning

confidence: 99%

Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficientPichia pastoris

Bustos,

Berrios,

Fickers

2024

Preprint

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“…The carbon source includes a variety of compounds such as glucose [18], glycerol P. pastoris grows optimally at 28-30 • C [16] with a tolerance of pH ranging from 3 to 7 [17]. The carbon source includes a variety of compounds such as glucose [18], glycerol [19], L-rhamnose [20], formate [21], and so on. This microbe can also use methanol as its sole source of carbon and energy owing to the presence of enzymes in the peroxisomes essential to methanol metabolism, such as alcohol oxidase, dihydroxyacetone synthase, and peroxidase [22].…”

Section: Physiological Characteristics Of Pichia Pastorismentioning

confidence: 99%

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Zha,

Liu,

Ren

et al. 2023

JoF

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Pichia pastoris is the most widely used microorganism for the production of secreted industrial proteins and therapeutic proteins. Recently, this yeast has been repurposed as a cell factory for the production of chemicals and natural products. In this review, the general physiological properties of P. pastoris are summarized and the readily available genetic tools and elements are described, including strains, expression vectors, promoters, gene editing technology mediated by clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9, and adaptive laboratory evolution. Moreover, the recent achievements in P. pastoris-based biosynthesis of proteins, natural products, and other compounds are highlighted. The existing issues and possible solutions are also discussed for the construction of efficient P. pastoris cell factories.

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Bioconversion of C1 feedstocks for chemical production using Pichia pastoris

Guo

Qiao

Xin

et al. 2023

Trends in Biotechnology

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Enhancing xylanase expression by Komagataella phaffii by formate as carbon source and inducer

Cited by 4 publications

References 39 publications

Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficientPichia pastoris

Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficientPichia pastoris

Advances in Metabolic Engineering of Pichia pastoris Strains as Powerful Cell Factories

Bioconversion of C1 feedstocks for chemical production using Pichia pastoris

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