Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I

Abstract

BackgroundThis study aimed to investigate the molecular structural relationship between cell adhesive molecule CD44 and Lewis y antigen, and determine the effects of Lewis y antigen on CD44-mediated adhesion and spreading of ovarian cancer cell line RMG-I and the Lewis y antigen-overexpressed cell line RMG-I-H.MethodsThe expression of CD44 in RMG-I and RMG-I-H cells before and after treatment of Lewis y monoclonal antibody was detected by immunocytochemistry; the expression of Lewis y antigen and CD44 was detected by Western Blot. The structural relationship between Lewis y antigen and CD44 was determined by immunoprecipitation and confocal laser scanning microscopy. The adhesion and spreading of RMG-I and RMG-I-H cells on hyaluronic acid (HA) were observed. The expression of CD44 mRNA in RMG-I and RMG-I-H cells was detected by real-time RT-PCR.ResultsImmunocytochemistry revealed that the expression of CD44 was significantly higher in RMG-I-H cells than in RMG-I cells (P < 0.01), and its expression in both cell lines was significantly decreased after treatment of Lewis y monoclonal antibody (both P < 0.01). Western Blot confirmed that the content of CD44 in RMG-I-H cells was 1.46 times of that in RMG-I cells. The co-location of Lewis y antigen and CD44 was confirmed by co-immunoprecipitation. The co-expression of CD44 and Lewis y antigen in RMG-I-H cells was 2.24 times of that in RMG-I cells. The adhesion and spreading of RMG-I-H cells on HA were significantly enhanced as compared to those of RMG-I cells (P < 0.01), and this enhancement was inhibited by Lewis y monoclonal antibody (P < 0.01). The mRNA level of CD44 in both cell lines was similar (P > 0.05).ConclusionLewis y antigen strengthens CD44-mediated adhesion and spreading of ovarian cancer cells.

Expression of Lewis y antigen and integrin αv, β3 in ovarian cancer and their relationship with chemotherapeutic drug resistance

“…Lewis y antigen is not only a part of the integrin α5β1 and αvβ3 structures, but is also a part of the structure of other adhesion molecules such as CD44 [19]. Therefore, increased expression of Lewis y antigen can improve the adhesion of cells to the matrix and promote cell adhesion and metastasis through corresponding signal transduction pathways.…”

G9a is essential for EMT-mediated metastasis and maintenance of cancer stem cell-like characters in head and neck squamous cell carcinoma

“…CD44, a CSC marker in HNSCC, plays a role in tumor metastasis [33]. CD44 is a cell-surface glycoprotein that functions as a receptor for hyaluronic acid and is involved in cell adhesion and migration [34]. In HNSCC, a subpopulation of CD44 + CSCs displays a phenotypic switch, and become either proliferative or migratory [35].…”

Chemoresistance Is Associated with MUC1 and Lewis y Antigen Expression in Ovarian Epithelial Cancers

“…Our previous studies found that an upregulated expression of the Lewis y antigen can increase the expression of CD44, α5β1 and αvβ3. The Lewis y antigen, a structural component of CD44, α5β1 and αvβ3, increases cell adhesion and chemoresistance through various signaling pathways [6,26]. The Lewis y antigen, an important structural component of many molecules, can increase the invasiveness, adhesion and anti-apoptotic nature of cancer cells, leading to an increased chemoresistance.…”