Enkephalinergic sympathetic and parasympathetic innervation of the rat submandibular and sublingual glands

Shida, Toru; Ueda, Yutaka; Ishida‐Yamamoto, Akemi; Senba, Emiko; Tohyama, Masaya

doi:10.1016/0006-8993(91)90354-x

Cited by 14 publications

(9 citation statements)

References 22 publications

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“…Indeed, the relation between neuronal size and the extension of its axonal terminals was described previously [38,42]. Several studies showed the possible cause of the differences in neuronal size between target specific populations [20,42,47,48]. Voyvodic [54,55] demonstrated that increasing the relative target area by partial denervation resulted in a significant increase in the diameter of the SCG neurons, while decreasing the relative target area resulted in a decrease in the diameter of neurons.…”

Section: Discussionmentioning

confidence: 77%

“…It is generally understood that the salivary secretion of the parotid gland (PG) which is composed almost entirely of serous acini and the submandibular gland (SMG) which is composed of a mixture of serous and mucous acini [31] are controlled by both the sympathetic and parasympathetic nervous systems, and secretion of the sublingual gland (SLG) which is composed of mainly mucous acini appears to be controlled by the parasympathetic nervous system alone [15,51,54]. However, the SLG is also innervated by the superior cervical ganglion (SCG) whose nerve fibers distribute around ducts or blood vessels and decrease blood flow in the SLG [48,52].…”

Section: Introductionmentioning

confidence: 99%

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Differences in Morphology and Neuropeptide Immunoreactivity of Superior Cervical Ganglion Neurons that Innervate the Major Salivary Glands in Rats.

Miyauchi

Asamoto

Nojyo

et al. 2001

Acta Histochem. Cytochem

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A neuronal tracer, WGA-HRP, was injected into the rat parotid gland (PG), submandibular gland (SMG), and sublingual gland (SLG) to label the superior cervical ganglion (SCG) neurons, and the number, size of soma, dendritic arborization and immunoreactivities (IR) of vasoactive intestinal polypeptide (VIP) and neuropeptide Y (NPY) were investigated. The number and size of neurons projecting into SMG were greater than those of neurons projecting into the PG or SLG. The PG and SLG projecting neurons did not show significant differences in their number and size of soma, even the weight of the SLG was much smaller than the other two glands. The total length of dendrites of the SMG projecting neurons was greater than those of the PG or SLG projecting neurons. PG and SLG projecting neurons showed no significant difference in the total length of their dendrites. PG and SLG projecting neurons with NPY-IR were larger in number than SMG projecting neurons. SLG projecting neurons with VIP-IR were greater in number than neurons projecting into the other two glands. Our results indicate the differences in morphology and neuropeptide immunoreactivities among the SCG neurons which may be due to the differences in the neuronal functions and the participation of neurotrophic factors.

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Section: Discussionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

Differences in Morphology and Neuropeptide Immunoreactivity of Superior Cervical Ganglion Neurons that Innervate the Major Salivary Glands in Rats.

Miyauchi

Asamoto

Nojyo

et al. 2001

Acta Histochem. Cytochem

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“…However, in contrast to the rat, in the monkey, MGn neurons stained only for NPY and SP (Ng et al 1995). The presence of enkephalin (ENK) and TH in neurons of the rat MGn was also observed by Shida et al (1991). Four types of neurons have been distinguished in the rat MGn: cells containing both TH and ENK (9% of all ganglion cells), cells containing only ENK (17%), cells containing only TH (4%) and cells lacking both ENK and TH.…”

Section: Discussionmentioning

confidence: 89%

“…It has been demonstrated in a variety of species including the rat, mouse, cat, ferret and humans that nerve fibres innervating the MGl may contain biologically active substances, including vesicular acetylcholine transporter (VAChT; cholinergic marker), neuronal nitric oxide synthase (nNOS), vasoactive intestinal polypeptide (VIP), pituitary adenylate cyclase activating peptide (PACAP), VIPrelated peptide histidine methionine (PHM), neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL), Met5-enkephalin (Met-ENK), substance P (SP) and calcitonin gene-related peptide (CGRP) (Soinila et al 1989;Tobin et al 1990;Shida et al 1991;Hauser-Kronberger et al 1992;Lohinai et al 1995;Tobin et al 1995;Alm et al 1997;Jia et al 1997;Kusakabe et al 1997;Takai et al 1999;Del Fiacco et al 2014). Although relatively much is known about the innervation of the MGl, it should be emphasised that the studies mentioned above have mostly focused on laboratory mammals and humans.…”

mentioning

confidence: 99%

“…Furthermore, studies on the neurochemical coding of neurons in the MGn are scarce and were performed exclusively on laboratory animals. Neurons in the rat, monkey and ferret MGn have been found to exhibit tyrosine hydroxylase (TH)-, nNOS-, VIP-, NPY-, ENK-, and SP-immunoreactivity (Shida et al 1991;Alm et al 1997;Takai et al 1999). Interestingly, only a very limited amount of information is available on the expression of cholinergic markers in these parasympathetic nerve cells (Schafer et al 1998).…”

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confidence: 99%

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Immunohistochemical characterisation of neurons in the mandibular ganglion and nerve fibres supplying the porcine mandibular gland

Klimczuk¹,

Podlasz²,

Sienkiewicz³

et al. 2016

Vet. Med. - Czech

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ABSTRACT:The present study was designed to investigate the chemical coding of neurons in the mandibular ganglion (MGn) and nerve fibres supplying the porcine mandibular gland (MGl) with the use of immunofluorescence and RT-PCR. The cryostat sections from MGn and MGl were processed for double-labelling immunohistochemistry using antisera against vesicular acetylcholine transporter (VAChT), choline acetyltransferase (ChAT), dopamine β-hydroxylase (DβH), neuronal nitric oxide synthase (nNOS), vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), galanin (GAL), substance P (SP) and calcitonin gene-related peptide (CGRP). The MGl was found to be richly supplied by VAChT-positive nerve fibres that surrounded intra-and interlobular salivary ducts. A large number of VAChT-immunoreactive (VAChT-IR) nerve terminals were also observed around acini. Many periductal and periacinar nerve fibres stained positive for DβH. Immunoreactivity to GAL, NPY or VIP was observed in an intermediate number of nerve terminals which were associated with both salivary ducts and acini. Double-immunostaining revealed that in MGn nearly all neurons stained positive for VAChT/ChAT (98.45 ± 0.59%, mean ± SEM) and nNOS (99.71 ± 0.18%). An intermediate number of the nerve cell bodies displayed immunoreactivity to NPY or VIP (18.67 ± 0.52% and 8.11 ± 0.36%, respectively). Single GAL-IR and CGRP-positive neurons were also observed. RT-PCR revealed the presence of transcripts of ChAT, VAChT, nNOS, NPY, VIP and GAL. For SP and DβH very weak signals were observed. RT-PCR with primers targeting CGRP did not generate any PCR product.

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Coexistence of enkephalin- and tyrosine hydroxylase-like immunoreactivities in nerve fibers of the temporomandibular joint capsule of the guinea pig

1998

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Background:The innervation of joints has been suggested to play an important role for their normal function as well as for the pathogenesis of chronic arthritic conditions. It is therefore important to elucidate the functional anatomy of this innervation.Methods: The expression of methionine enkephalin-like immunoreactivity (ENK-LI) and tyrosine hydroxylase (TH)-LI as well as their possible colocalization were examined in the temporomandibular joint of the guinea pig.Results: Nerve fibers with ENK-LI were demonstrated in the synovium of the guinea pig temporomandibular joint capsule with the use of indirect immunofluorescence. The ENK ؉ fibers were found close to the surface of the synovial membrane, deeper in the synovium, and at the walls of blood vessels. Coexistence of ENK-and TH-LI was observed frequently in the synovial nerve fibers. After removal of the superior cervical ganglion (SCG), the ENKcontaining nerve fibers were reduced substantially in number on the operated side or were completely absent if the cervical sympathetic trunk was also excised.Conclusions: The findings indicate that the majority of fibers with ENK-LI derive from the SCG. ENK may act as a neuromodulator on the catecholaminergic nerves and may also have an analgesic effect in the joint.

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Enkephalinergic sympathetic and parasympathetic innervation of the rat submandibular and sublingual glands

Cited by 14 publications

References 22 publications

Differences in Morphology and Neuropeptide Immunoreactivity of Superior Cervical Ganglion Neurons that Innervate the Major Salivary Glands in Rats.

Differences in Morphology and Neuropeptide Immunoreactivity of Superior Cervical Ganglion Neurons that Innervate the Major Salivary Glands in Rats.

Immunohistochemical characterisation of neurons in the mandibular ganglion and nerve fibres supplying the porcine mandibular gland

Coexistence of enkephalin- and tyrosine hydroxylase-like immunoreactivities in nerve fibers of the temporomandibular joint capsule of the guinea pig

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