Enoxacin inhibits proliferation and invasion of human osteosarcoma cells and reduces bone tumour volume in a murine xenograft model

Abstract

Osteosarcoma is the most prevalent primary bone malignancy in children and adolescents. Neoadjuvant chemotherapy combined with surgical resection, the current standard treatment of osteosarcoma, is associated with a 5-year survival rate of only ~70%. Therefore, it is necessary to identify new, more effective treatment strategies for patients with this lethal disease. Enoxacin is a highly effective broad-spectrum fluoroquinolone antibiotic with low toxicity. The drug inhibits the growth and metastasis of numerous tumour types, but its efficacy has not been studied in osteosarcoma. This study assessed the antitumour effects of enoxacin in osteosarcoma 143B cells and in a murine tumour xenograft model. Enoxacin inhibited the proliferation, invasion and migration of 143B cells, as well as inducing their apoptosis. These effects were thought to be mediated by downregulation of Bcl-xL, Bxl-2, matrix metalloproteinase (MMP)2 and MMP9 expression. Enoxacin also significantly impaired the growth of bone tumours in nude mice without affecting their liver or kidney function, or blood cell count. Collectively, these results indicate that enoxacin is a promising new drug for osteosarcoma that warrants further evaluation in clinical studies.

The role of genes of the Argonaute family in the effects of the RNA interference activator enoxacin on the lifespan of Drosophila melanogaster

“…Эноксацин является многообещающим средством для лечения некоторых заболеваний, в том числе связанных со старением. Он ингибирует рост многих типов раковых клеток in vitro и in vivo, включая остеосаркому [36], меланому [37], рак предстательной железы [26], поджелудочной железы [38], легкого [39], щитовидной железы [40], шейки матки [41]. Кроме того, это вещество останавливает прогрессирование аутоиммунного процесса в тканях желчевыводящих путей [42], а также уменьшает вызванное диетой (с высоким содержанием жиров 60 %) ожирение у мышей, нормализует уровень глюкозы в крови и снижает симптомы бокового амиотрофического склероза [43,44].…”

The RNAi enhancer enoxacin inhibits the growth and migration of esophageal squamous cell carcinoma cells

“…As shown in Table 1, in vitro and in vivo investigations on various types of cancer using several members of this family of antibiotics have shown a signi cant inhibitory effect on cancer cell growth and proliferation. Among all uoroquinolones tested, enoxacin has frequently been found to have the most potent inhibitory effect on cancer cells 12,13 . It was revealed that the anti-cancer effects of enoxacin is exerted through the general enhancement of the RNA interference (RNAi) pathway, thereby stimulating the miRNA biogenesis pathway 14 .…”

Application of interpretable machine learning algorithms to predict distant metastasis in osteosarcoma

“…Osteosarcoma is widely acknowledged as the most common type of primary malignant bone tumor that arises from mesenchymal tissue, 1 with an incidence rate of 4 to 5 cases per million people. 2 It has a bimodal age distribution pattern, occurring predominantly in children and young adults <20 years of age and those >50-60 years of age. 3,4 It has been reported that metastasis disease is found in approximately 20% of osteosarcoma patients at diagnosis.…”