Enriching Rotifers with “Premium” Microalgae: Rhodomonas lens

Coutinho, Paula; Ferreira, Martiña; Otero, Ana

doi:10.1007/s10126-019-09936-4

Cited by 12 publications

(6 citation statements)

References 68 publications

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“…in this study were palmitic (C16:0), linoleic (C18:2), linolenic (C18:3), and stearidonic (C18:4) acid. These FA's are in line with the literature results for Rhodomonas species (van Houcke et al, 2017;Vu et al, 2019;Coutinho et al, 2020). However, differ from lipid profiles of other microalgae that are used in aquaculture, for example, Tisochrysis lutea, Chaetoceros neogracile, Skeletonema marinoı, Pavlova lutheri, Nitzschia sp., Thalassiosira sp.…”

Section: Fatty Acid Compositionsupporting

confidence: 89%

“…PUFAs, which are the most important FA for aquaculture, indicated a 2-fold increase in the first 96 h of N-starvation. Similar increased PUFA content (65% of total FA) was reported by Coutinho et al (2020) for Rhodomonas lens maintained under nitrate saturated conditions. DHA and EPA presented a peak in concentration 72 h after N-starvation.…”

Section: Fa Concentrationsupporting

confidence: 85%

“…Rhodomonas sp. has a high content of essential fatty acids (PUFAs) and nutrients, lipid accounts for about 10-30%, and protein takes about 30-60% of the dry weight (Renaud et al, 1999;Seixas et al, 2009;Costard et al, 2012;Coutinho et al, 2020). The variability of lipid and protein concentration depends on cultivation conditions.…”

Section: Introductionmentioning

confidence: 99%

“…It has been previously proved to constitute a high-quality diet to rear calanoid copepods Acartia sinjiensis and Artemia sp. (Knuckey et al, 2005;Seixas et al, 2009;Coutinho et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Nitrogen Starvation on Biomass Yield and Biochemical Constituents of Rhodomonas sp.

2020

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The microalgae Rhodomonas sp. is known as an excellent feed source for live feed organisms such as copepods. The main benefits of feeding Rhodomonas to live feed animals are attributed to the relative high polyunsaturated fatty acid (PUFA) level, the combination of containing both docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), and the ratio between these fatty acids (FA). It has been shown that microalgae are able to accumulate valuable metabolites, such as lipids, under adverse conditions. The easiest and most inexpensive method to induce stress to microalgae is through nitrogen (N) starvation. In this study, the effect of N-starvation on biomass concentration, cell volume, and cellular composition, such as fatty acid concentration and composition, and phycoerythrin (PE) concentration of Rhodomonas sp. during a period of 8 days, was investigated. The research was divided into two stages. In the first (growth) stage, Rhodomonas sp. was cultivated in small 400 ml photobioreactors (Algaemist-S) under optimal conditions in turbidostat mode, which reached a biomass concentration of 1.5 gDW L −1 and dilution rate of 1.3 d −1. Samples were taken every 24 h for cell density and volume and productivity measurements in order to ensure a healthy and stable culture. In the next stage (N-starvation), the biomass was washed and transferred in a reactor filled with N-depleted medium. During N-starvation, samples were taken for biomass concentration, cell volume, PE and FA composition. The results of this study demonstrate that the lipid content increased significantly from 9% (t = 0 h) to 30% (t = 120 h) of the dry weight. After 120 h of N-starvation, the total FA content of Rhodomonas sp. remained stable for the remainder of the experiment (next 72 h). The highest increase of the FA concentration was represented by C16:0, C:18:1, C18:2, and C18:3, with highest concentrations after 120 h of starvation. The maximum EPA and DHA concentrations were observed after 48 h of starvation, while the maximum DHA to EPA ratio was detected at the end of the starvation.

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Section: Fatty Acid Compositionsupporting

confidence: 89%

Section: Fa Concentrationsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

“…It has been previously proved to constitute a high-quality diet to rear calanoid copepods Acartia sinjiensis and Artemia sp. (Knuckey et al, 2005;Seixas et al, 2009;Coutinho et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Nitrogen Starvation on Biomass Yield and Biochemical Constituents of Rhodomonas sp.

2020

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“…Most aquatic animals are not able to synthesize polyunsaturated fatty acids (PUFA) which are crucial for their growth and development (Kanazawa et al 1979;Langdon and Waldock 1981;Gonzalez Araya et al 2012). Rhodomonas salina is an excellent feed because of its relatively high PUFA levels (> 60% of total fatty acids) and the combination of eicosapentaenoic acid (EPA 20:5n-3, 8% of total fatty acids) and docosahexaenoic acid (DHA 22:6n-3, 3-7% of total fatty acids) (Renaud et al 1999;Seixas et al 2009;Coutinho et al 2020). The increased growth and larvae survival are attributed to the increased concentrations of EPA and DHA in their diets (Thompson et al 1993;Helm and Bourne 2004;Brown and Blackburn 2013).…”

Section: Introductionmentioning

confidence: 99%

Effect of pH on Rhodomonas salina growth, biochemical composition, and taste, produced in semi-large scale under sunlight conditions

et al. 2022

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Rhodomonas salina is a microalgal species, belonging to the cryptophytes, and is widely used as aquaculture feed because of its high nutritional profile and phycoerythrin content. This study investigated the effect of pH on the growth, biochemical composition, and taste of R. salina when cultivated on a semi-large scale under natural light conditions. Two tubular photobioreactors (200 L) were used for the cultivation of R. salina with sunlight as the only illumination source. Two different pH setpoints were applied, 7 and 8.5. Optimal temperature and nutrient conditions were applied, according to previous research findings. The results demonstrated that the productivity of R. salina was higher at pH 7, 0.06–0.14 gdry weight L−1 day−1, compared to pH 8.5, 0.03–0.12 gdry weight L−1 day−1. It was found that protein and total fatty acid concentrations were higher in the biomass that was produced at pH 8.5, 33.7% and 12.3% of dry weight, respectively, while at pH 7, the protein content was 31.9% and the total fatty acids 8.8% of dry weight. The phycoerythrin concentration, like protein, was higher at pH 8.5, 2.7% of dry weight, compared to pH 7, 1% of dry weight. The free amino acid and nucleotide profile of R. salina was affected by the pH, resulting in increased equivalent umami concentration at pH 7. For the sensory evaluation, an expert panel on algae flavors evaluated the effect of pH on the taste of R. salina, reporting that the biomass that was produced at pH 7 had more umami flavor than the biomass that was produced at pH 8.5, which was evaluated as more bitter.

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