Enrichment and biofilm formation of Anammox bacteria in a non-woven membrane reactor

Ni, Shou-Qing; Lee, Po‐Heng; Fessehaie, Anania; Bao, Gao; Sung, Shihwu

doi:10.1016/j.biortech.2009.10.050

Cited by 93 publications

(45 citation statements)

References 33 publications

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“…The anammox bacterial enrichment was observed in terms of operational parameters (pH, Temperature), nitrogen transformations, biomass development, and the presence of trace amounts of intermediates (van Dongen et al 2001). The confirmation of anammox bacteria was carried out by SEM analysis and using molecular techniques (Arrojo et al 2006;Ni et al 2010). The pH of the AnAOB reactors observed was as follows: , and M AN-AOB -7.2-7.6 (Guven et al 2004).…”

Section: Confirmation Studies For Aob (Mpn Analysis)mentioning

confidence: 98%

“…The physical nature and the surface morphology of the anaerobic reactor samples were determined during the end of the study period using scanning electron microscopy (Arrojo et al 2006;Ni et al 2010 The identification of the anammox bacteria in M AnAOB reactor was carried out by molecular techniques. DNA was extracted from biomass samples of M AnAOB reactor using QIAmp DNA mini kit (Qiagen).…”

Section: Scanning Electron Microscopy (Sem) Image Analysismentioning

confidence: 99%

“…The morphology and inner structure of the enriched AnAOB biomass in the 100-mL reactors were observed in more detail with the scanning electron microscopy [Photographs are as shown in Online Resource 1 (SEM_FC, MC, SC, and LC)]. The SEM images of the enriched AnAOB biomass in the reactors showed were mostly spherical and elliptical bacteria with single cells and clusters having a rough surface which were interspersed (Arrojo et al 2006;Ni et al 2010). There were also presence of few other organisms by the identification of different other structures like filamentous, tubular and rod-shaped bacteria, indicating the coexistence of anammox bacteria with other microbial populations like AOBs, NOBs, and denitrifiers in FC, MC, SC, and LC reactors (Wang et al 2009).…”

Section: Anammox Enrichment In 100-ml Reactorsmentioning

confidence: 99%

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Aerobic and anaerobic ammonium-oxidising bacterial enrichment from municipal solid waste

Shalini

Kurian

Wong

2014

Int. J. Environ. Sci. Technol.

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Leachate from the municipal solid waste (MSW) landfills contains high concentration of ammoniacal nitrogen that is a major toxic pollutant that has a great threat to environment. Among the processes available for the removal of ammoniacal nitrogen, one process is a combination of partial nitrification and anaerobic ammonium oxidation (anammox) process. It requires aerobic ammonium-oxidising bacteria (AOB) and anaerobic ammonium-oxidising bacteria (AnAOB). This paper presents the feasibility of enriching the AOB and AnAOB in 100-mL and 2.5-L batch reactors from fresh and mined MSW and leachate under aerobic and anaerobic conditions with varying feed-to-seed ratio. The AOB and AnAOB activity was monitored by measuring the intermediates such as hydroxylamine and hydrazine along with variations in ammoniacal nitrogen, nitrite nitrogen, and nitrate nitrogen concentrations in the reactor contents. The formation of intermediates such as hydroxylamine and hydrazine and ammoniacal nitrogen transformation data confirmed the enrichment of AOB and AnAOB. Further, AOB and AnAOB were validated by most probable number test and scanning electron microscopy analysis, respectively. DNA extraction, polymerase chain reaction amplification, and sequencing analysis authenticated the AnAOB as Candidatus Brocadia anammoxidans.

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Section: Confirmation Studies For Aob (Mpn Analysis)mentioning

confidence: 98%

Section: Scanning Electron Microscopy (Sem) Image Analysismentioning

confidence: 99%

Section: Anammox Enrichment In 100-ml Reactorsmentioning

confidence: 99%

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Aerobic and anaerobic ammonium-oxidising bacterial enrichment from municipal solid waste

Shalini

Kurian

Wong

2014

Int. J. Environ. Sci. Technol.

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“…The content of the biofilm in the suspended biomass has a significant impact on the suspended biomass sorption capacity. Biofilm structure and the extracellular polymeric substance (EPS) content increase the possibility of ion sorption (Guibaud et al, 2009;Ozturk et al, 2009;Ni et al, 2010). EPS is polyanionic in nature, with enhanced capability for cation ion bonding.…”

Section: +mentioning

confidence: 99%

Biosorption of Mn (II), Co (II) and Cr (VI) in a horizontal rotating tubular bioreactor: experiments and evaluation of the integral bioprocess model

Rezić

Zeiner

et al. 2014

Braz. J. Chem. Eng.

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-In this research, a multi heavy metals removal process with mixed microbial culture was examined in a horizontal rotating tubular bioreactor (HRTB) with different combinations of process parameters. Three metals were selected as examples of cations (manganese and cobalt) and oxy-anion (hexavalent chromium). Hydrodynamic conditions and biomass sorption capacity in the HRTB had the main impact on the heavy metals removal efficiencies, which were for Mn 2+ 87.0-93.6%, Co 2+ 89.0-95.7% and Cr 6+ 99.7-100%, respectively. For the bioprocess description in the HRTB, the integral bioprocess model that combines hydrodynamics, mass transfer and kinetics was used. This model was evaluated for the new experimental conditions and average variances between experimental and simulated data were in the range of 0.12 -3.21·10 -3 . The results obtained clearly show that the integral bioprocess model is able to describe the heavy metal removal process in the HRTB.

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“…(1)), the conversion factor (Y) is 0.066 molCH 2 O 0,5 N 0,15 /mol NH 4 + . The low conversion rate of substrate into biomass reduces the costs with treatment and final disposal of the sludge (NI et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Establishment of anammox process in sludge samples collected from swine wastewater treatment system

et al. 2011

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ABSTRACT:The high load of nitrogen present in swine wastewater is one of the biggest management challenges of the activity. The Anammox process emerges as a good alternative for biological removal of nitrogen. This study aims to acclimate sludge collected from swine effluent treatment systems to establish the Anammox process. Two sludge samples were collected at Embrapa Swine and Poultry, Concordia -SC, Brazil, one from the bottom of an inactive anaerobic pond (inoculum A) and another from an aeration tank (inoculum B). Both were acclimated until the depletion of NO 3 -N, being subsequently inoculated in two reactors (Reactor A -Inoculum A and Reactor B -Inoculum B). The Reactor A showed activity after 110 days of operation, while the Reactor B needed 170 days. The difference in the start-up time could be explained by the different environmental conditions to which each sludge was submitted. FISH and PCR analyses confirmed the presence of microorganisms with Anammox activity, demonstrating that the sludge of swine wastewater treatment systems is a good source of inoculum for the development of the Anammox process.KEYWORDS: nitrogen removal, swine wastewater, Anammox process. ESTABELECIMENTO DO PROCESSO ANAMMOX A PARTIR DE LODO DE SISTEMA DE TRATAMENTO DE EFLUENTE DA SUINOCULTURARESUMO: A elevada carga de nitrogênio presente em efluentes da suinocultura é um dos maiores desafios de manejo da atividade. O processo Anammox surge como boa alternativa para a remoção biológica desse nutriente. Este trabalho teve como objetivo aclimatar diferentes amostras de lodos de sistemas de tratamento de efluentes da suinocultura com vistas ao estabelecimento do processo Anammox. Dois inóculos foram coletados na Embrapa Suínos e Aves, Concórdia -SC, um dos quais no fundo de uma lagoa anaeróbia inativa (Inóculo A) e o outro, em um tanque de aeração (Inóculo B). Ambos foram aclimatados até cessar o consumo de N-NO 3 -e, posteriormente, inoculados em dois reatores (Reator A -Inóculo A e Reator B -Inóculo B). O Reator A apresentou atividade, após aproximadamente 110 dias de operação, enquanto o Reator B precisou de aproximadamente 170 dias, diferença de tempo de partida que pode ser explicada pelas diferentes condições ambientais a que cada lodo estava submetido. As análises de FISH e PCR comprovaram a presença de microrganismos com atividade Anammox, mostrando que lodos de sistemas de tratamento de efluentes da suinocultura podem ser boa fonte de inóculo para o desenvolvimento do processo Anammox. PALAVRAS-CHAVE: remoção de nitrogênio, dejetos de suínos, anammox.Establishment of anammox process in sludge samples collected from swine wastewater treatment system Eng.

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Enrichment and biofilm formation of Anammox bacteria in a non-woven membrane reactor

Cited by 93 publications

References 33 publications

Aerobic and anaerobic ammonium-oxidising bacterial enrichment from municipal solid waste

Aerobic and anaerobic ammonium-oxidising bacterial enrichment from municipal solid waste

Biosorption of Mn (II), Co (II) and Cr (VI) in a horizontal rotating tubular bioreactor: experiments and evaluation of the integral bioprocess model

Establishment of anammox process in sludge samples collected from swine wastewater treatment system

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