Enrichment and characterization of a highly efficient tetrahydrofuran-degrading bacterial culture

Huang, Hui; H, Yu; Qi, Minbo; Liu, Zubi; Wang, Haixia; Lü, Zhenmei

doi:10.1007/s10532-019-09888-5

Cited by 15 publications

(15 citation statements)

References 58 publications

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“…The strains and plasmids used in this study are listed in Table 1. The THF-degrading strain Rhodococcus ruber ZM07 Król et al, 2010 (collection number CCTCC AB 2019217) was isolated from an enrichment culture designated H-1 (Huang et al, 2019). The non-THF-degrading strain Escherichia coli K12 (Bachmann et al, 1976) and its knockout strain K12 thiE (which can neither degrade THF nor produce thiamine) were both cocultured with ZM07, and the latter strain was constructed as described in the Supplementary Methods.…”

Section: Strains and Plasmidsmentioning

confidence: 99%

“…For experiments under thiamine-rich conditions, 0.01 mM external thiamine was added to the BSM. All strains used in this study were cultured in 100 mL of BSM with an initial pH of 7.0 at 30 • C, referred to the conditions of THF-degrading bacterial culture H-1 (Huang et al, 2019). Most experiments were performed using 500 mL shaking flasks at 160 rpm, while specifically designed two-phase reactors were used in the experiment to verify the interaction mode between strains ZM07 and K12 with a shaking rate of 100 rpm.…”

Section: Subculture Conditions and Experimentsmentioning

confidence: 99%

“…In this study, a THF-degrading bacterium, Rhodococcus ruber ZM07, was isolated from a THF-degrading bacterial culture H-1 (Huang et al, 2019). This strain cannot be continuously passaged in basal salt medium (BSM) with THF as the sole carbon source, although it can cooperate with many kinds of other bacteria, such as Escherichia coli K12, to form a stable symbiotic system.…”

Section: Introductionmentioning

confidence: 99%

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Thiamine-Mediated Cooperation Between Auxotrophic Rhodococcus ruber ZM07 and Escherichia coli K12 Drives Efficient Tetrahydrofuran Degradation

Huang

Liu

et al. 2020

Front. Microbiol.

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Tetrahydrofuran (THF) is a universal solvent widely used in the synthesis of chemicals and pharmaceuticals. As a refractory organic contaminant, it can only be degraded by a small group of microbes. In this study, a thiamine auxotrophic THF-degrading bacterium, Rhodococcus ruber ZM07, was isolated from an enrichment culture H-1. It was cocultured with Escherichia coli K12 (which cannot degrade THF but can produce thiamine) and/or Escherichia coli K12ΔthiE (which can neither degrade THF nor produce thiamine) with or without exogenous thiamine. This study aims to understand the interaction mechanisms between ZM07 and K12. We found that K12 accounted for 30% of the total when cocultured and transferred with ZM07 in thiamine-free systems; in addition, in the three-strain (ZM07, K12, and K12ΔthiE) cocultured system without thiamine, K12ΔthiE disappeared in the 8th transfer, while K12 could still stably exist (the relative abundance remained at approximately 30%). The growth of K12 was significantly inhibited in the thiamine-rich system. Its proportion was almost below 4% after the fourth transfer in both the two-strain (ZM07 and K12) and three-strain (ZM07, K12, and K12ΔthiE) systems; K12ΔthiE’s percentage was higher than K12’s in the three-strain (ZM07, K12, and K12ΔthiE) cocultured system with exogenous thiamine, and both represented only a small proportion (less than 1% by the fourth transfer). The results of the coculture of K12 and K12ΔthiE in thiamine-free medium indicated that intraspecific competition between them may be one of the main reasons for the extinction of K12ΔthiE in the three-strain (ZM07, K12, and K12ΔthiE) system without exogenous thiamine. Furthermore, we found that ZM07 could cooperate with K12 through extracellular metabolites exchanges without physical contact. This study provides novel insight into how microbes cooperate and compete with one another during THF degradation.

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Section: Strains and Plasmidsmentioning

confidence: 99%

Section: Subculture Conditions and Experimentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thiamine-Mediated Cooperation Between Auxotrophic Rhodococcus ruber ZM07 and Escherichia coli K12 Drives Efficient Tetrahydrofuran Degradation

Huang

Liu

et al. 2020

Front. Microbiol.

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“…Additionally, strain P. dioxanivorans CB1190 was shown to degrade 120 mg/L 1,4-dioxane with a degradation rate of 30.23 ± 2.86 mg L −1 d −1 , which decreased by nearly 90% with the addition of 0.15 and 0.3 mM Cu 2+ [ 45 ]. In addition, Cu 2+ was also observed to sharply inhibit the THF degradation by consortium H1, with a THF degradation ratio of less than 10% observed after 108 h when the Cu 2+ concentration was increased to 1 mM [ 47 ]. However, the THF degradation ratio was maintained at 51.3% and was only 12% lower than that of the normal control group when strain ZM01 was cultivated in the presence of 1 mM Cu 2+ , indicating that it has better THF degradation potential under Cu 2+ stress.…”

Section: Resultsmentioning

confidence: 99%

Biodegradation of Tetrahydrofuran by the Newly Isolated Filamentous Fungus Pseudallescheria boydii ZM01

Ren

Wang

et al. 2020

Microorganisms

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Tetrahydrofuran (THF) is widely used as a precursor for polymer syntheses and a versatile solvent in industries. THF is an environmental hazard and carcinogenic to humans. In the present study, a new THF-degrading filamentous fungus, Pseudallescheria boydii ZM01, was isolated and characterized. Strain ZM01 can tolerate a maximum THF concentration of 260 mM and can completely degrade 5 mM THF in 48 h, with a maximum THF degradation rate of 133.40 mg THF h−1 g−1 dry weight. Growth inhibition was not observed when the initial THF concentration was below 150 mM, and the maximum THF degradation rate was still maintained at 118.21 mg THF h−1 g−1 dry weight at 50 mM THF, indicating the great potential of this strain to degrade THF at high concentrations. The initial key metabolic intermediate 2-hydroxytetrahydrofuran was detected and identified by gas chromatography (GC) analyses for the first time during the THF degradation process. Analyses of the effects of initial pH, incubation temperature, and heavy metal ions on THF degradation revealed that strain ZM01 can degrade THF under a relatively wide range of conditions and has good degradation ability under low pH and Cu2+ stress, suggesting its adaptability and applicability for industrial wastewater treatment.

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“…One liter of LB contained 10 g tryptone, 5 g yeast extract, and 10 g NaCl, and the initial pH of the media was 7.4. (Parales et al, 1994;Huang et al, 2019). For experiments under thiamine-rich conditions, 0.01 mM external thiamine was added to the BSM.…”

Section: Subculture Conditions and Experimentsmentioning

confidence: 99%

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Enrichment and characterization of a highly efficient tetrahydrofuran-degrading bacterial culture

Cited by 15 publications

References 58 publications

Thiamine-Mediated Cooperation Between Auxotrophic Rhodococcus ruber ZM07 and Escherichia coli K12 Drives Efficient Tetrahydrofuran Degradation

Thiamine-Mediated Cooperation Between Auxotrophic Rhodococcus ruber ZM07 and Escherichia coli K12 Drives Efficient Tetrahydrofuran Degradation

Biodegradation of Tetrahydrofuran by the Newly Isolated Filamentous Fungus Pseudallescheria boydii ZM01

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