Enrichment and characterization of a nitric oxide-reducing microbial community in a continuous bioreactor

Garrido-Amador, Paloma; Stortenbeker, Niek; Wessels, Hans; Speth, Daan R.; García-Heredia, Inmaculada; Kartal, Boran

doi:10.1038/s41564-023-01425-8

Cited by 7 publications

(2 citation statements)

References 97 publications

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“…On the other hand, the diversity increased for both cultivable bacterial and fungal communities when transferred to the high-AQI site compared to those maintained at the low-AQI site, indicating that increased NO and/or decreased O 3 promoted the growth of microorganisms unable to grow at the low-AQI site. This increase in diversity can be explained primarily by the growth of O 3 -sensitive microorganisms but also by the finding that some microbial taxa are capable of harnessing energy through the reduction of NO [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Environmental Gaseous Pollutants on the Cultivable Bacterial and Fungal Communities of the Aerobiome

Mejías,

Madrid,

Díaz

et al. 2024

Microorganisms

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Understanding air microbial content, especially in highly polluted urban areas, is crucial for assessing its effect on human health and ecosystems. In this context, the impact of gaseous pollutants on the aerobiome remains inconclusive due to a lack of studies separating this factor from other contaminants or environmental factors. In this study, we aimed to experimentally assess the influence of contrasting concentrations of atmospheric gaseous pollutants as isolated variables on the composition of the aerobiome. Our study sites were contrasting Air Quality Index (AQI) sites of the Metropolitan Region of Chile, where nitric oxide (NO) was significantly lower at the low-AQI site than at the high-AQI site, while ozone (O3) was significantly higher. Cultivable aerobiome communities from the low-AQI site were exposed to their own pollutants or those from the high-AQI site and characterized using high-throughput sequencing (HTS), which allowed comparisons between the entire cultivable communities. The results showed increased alpha diversity in bacterial and fungal communities exposed to the high-AQI site compared to the low-AQI site. Beta diversity and compositional hierarchical clustering analyses revealed a clear separation based on NO and O3 concentrations. At the phylum level, four bacterial and three fungal phyla were identified, revealing an over-representation of Actinobacteriota and Basidiomycota in the samples transferred to the high-AQI site, while Proteobacteria were more abundant in the community maintained at the low-AQI site. At the functional level, bacterial imputed functions were over-represented only in samples maintained at the low-AQI site, while fungal functions were affected in both conditions. Overall, our results highlight the impact of NO and/or O3 on both taxonomic and functional compositions of the cultivable aerobiome. This study provides, for the first time, insights into the influence of contrasting pollutant gases on entire bacterial and fungal cultivable communities through a controlled environmental intervention.

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Section: Discussionmentioning

confidence: 99%

The Impact of Environmental Gaseous Pollutants on the Cultivable Bacterial and Fungal Communities of the Aerobiome

Mejías,

Madrid,

Díaz

et al. 2024

Microorganisms

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“…[30] NO is also important for biofilm regulation not only in a clinical context [31] but also for biofouling or biofilm reactors, which are rapidly emerging in microbiology, bioengineering, materials science, and process engineering. [32][33][34][35][36] Because NO is a reactive gaseous molecule, various NO-donors with different release mechanisms, such as heat, enzymes, oxidants, reductants, nucleophiles, and light were explored. [37] Among these release stimuli, light represents the most elegant trigger.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Control Over Nitric Oxide Photorelease via a Molecular Keypad Lock

Elbeheiry,

Schulz

2024

Chemistry A European J

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Based on Boolean logic, molecular keypad locks secure molecular information, typically with an optical output. Here we investigate a rare example of a molecular keypad lock with a chemical output. To this end, the light‐activated release of biologically important nitric oxide from a ruthenium complex is studied, using proton concentration and photon flux as inputs. In a pH‐dependent equilibrium, a nitritoruthenium(II) complex is turned into a nitrosylruthenium(II) complex, which releases nitric oxide under irradiation with visible light. The precise prediction of the output nitric oxide concentration as function of the pH and photon flux is achieved with an artificial intelligence approach, namely the adaptive neuro‐fuzzy inference system. In this manner an exceptionally high level of control over the output concentration is obtained. Moreover, the provided concept to lock a chemical output as well as the output prediction may be applied to other (photo)release schemes.

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Determination of site‐specific nitrogen cycle reaction kinetics allows accurate simulation of in situ nitrogen transformation rates in a large North American estuary

Tang,

Fortin,

Intrator

et al. 2024

Limnology & Oceanography

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Nitrogen (N) bioavailability affects phytoplankton growth and primary production in the aquatic environment. N bioavailability is partly determined by biological N cycling processes that either transform N species or remove fixed N. Reliable estimates of their kinetic parameters can help understand the distribution of N cycling processes. However, available estimates of kinetic parameters are often derived from microbial isolates and may not be representative of the natural environment. Observations are particularly lacking in estuarine and coastal waters. We conducted isotope tracer addition incubations to evaluate substrate affinities of nitrification, denitrification and anammox in the Chesapeake Bay water column. The half‐saturation constant for ammonia oxidation ranged from 0.38 to 0.75 μM ammonium, substantially higher than observed in the open oceans. Half‐saturation constants for denitrification—0.92–1.86 μM nitrite or 1.15 μM nitrate—were within the lower end or less than those reported for other aquatic environments and for denitrifier isolates. Interestingly, water column denitrification potential was comparable to that of sedimentary denitrification, highlighting the contribution of the water column to N removal during anoxia. Mostly undetectable anammox rates prevented us from deriving the half‐saturation constants, suggesting a low affinity of anammox. Using these substrate kinetics, we were able to predict in situ N cycling rates and explain the vertical distribution of N nutrient concentrations. Our newly derived substrate kinetics parameters can be useful for improving model representation of N nutrient dynamics in estuarine and coastal waters, which is critical for assessing the ecosystem productivity and function.

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Enrichment and characterization of a nitric oxide-reducing microbial community in a continuous bioreactor

Cited by 7 publications

References 97 publications

The Impact of Environmental Gaseous Pollutants on the Cultivable Bacterial and Fungal Communities of the Aerobiome

The Impact of Environmental Gaseous Pollutants on the Cultivable Bacterial and Fungal Communities of the Aerobiome

Enhancing Control Over Nitric Oxide Photorelease via a Molecular Keypad Lock

Determination of site‐specific nitrogen cycle reaction kinetics allows accurate simulation of in situ nitrogen transformation rates in a large North American estuary

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