Enrichment and cultivation of pelagic bacteria from a humic lake using phenol and humic matter additions

Hutalle-Schmelzer, Kristine Michelle L.; Zwirnmann, Elke; Krüger, Angela; Grossart, Hans‐Peter

doi:10.1111/j.1574-6941.2010.00831.x

Cited by 18 publications

(26 citation statements)

References 54 publications

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“…Thus, it is assumed that only a specific fraction of natural bacterial communities can access the vast pool of organic polymers. Consistent with this, in many freshwater ecosystems, estuaries and coastal areas (representing the most productive systems and up to 20% of the surface waters on earth, and where most of the OM is directly accessible to only a very limited number of highly specialized bacteria) there appears to be a direct link between OM quality and bacterial community composition (Hutalle-Schmelzer et al 2010). Bacteria in such communities frequently form syntrophic associations and/or co-metabolism with other species (McInerney et al 2008), indicating that inter-specific interactions impact the functioning of the entire ecosystem (Keller & Surette 2006).…”

Section: Introductionmentioning

confidence: 75%

“…We also tested community-level responses to the introduction of an additional bacterial species that we expected to be competitively dominant under these conditions: Burkholderia, common in conditions with abundant polymeric OM (Hutalle-Schmelzer et al 2010) and potentially able to express chitino- (Kong et al 2001) and cellulolytic activity (Liang et al 2014). We found that community-level responses to predation differed from those of individual species, and that introduction of a protozoan grazer can enhance bacterial degradation of both chitin and cellulose by inducing bacterial co-aggregation.…”

Section: Introductionmentioning

confidence: 99%

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Interspecific interactions drive chitin and cellulose degradation by aquatic microorganisms

Corno

Salka²,

Pohlmann³

et al. 2015

Aquat. Microb. Ecol.

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Complex biopolymers (BPs) such as chitin and cellulose provide the majority of organic carbon in aquatic ecosystems, but the mechanisms by which communities of bacteria in natural systems exploit them are unclear. Previous degradation experiments in artificial systems predominantly used microcosms containing a single bacterial species, neglecting effects of interspecific interactions. By constructing simplified aquatic microbial communities, we tested how the addition of other bacterial species, of a nanoflagellate protist capable of consuming bacteria, or of both, affect utilization of BPs. Surprisingly, total abundance of resident bacteria in mixed communities increased upon addition of the protist. Concomitantly, bacteria shifted from free-living to aggregated morphotypes that seemed to promote utilization of BPs. In our model system, these interactions significantly increased productivity in terms of overall bacterial numbers and carbon transfer efficiency. This indicates that interactions on microbial aggregates may be crucial for chitin and cellulose degradation. We therefore suggest that interspecific microbial interactions must be considered when attempting to model the turnover of the vast pool of complex biopolymers in aquatic ecosystems.

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Section: Introductionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Interspecific interactions drive chitin and cellulose degradation by aquatic microorganisms

Corno

Salka²,

Pohlmann³

et al. 2015

Aquat. Microb. Ecol.

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show abstract

“…Two recent reports have also linked Methylophilus to utilization of biphenyl (Uhlik et al, 2009), phenol, and humic matter (Hutalle-Schmelzer et al, 2010). Interestingly, these compounds have a common phenolic hydroxyl proposed as intermediate compounds in anthracene degradation pathways (Pinyakong et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

Zhang

Wang

Wan

et al. 2011

J. Zhejiang Univ. Sci. B

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Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in a municipal solid waste (MSW) composting site. Knowledge of changes in microbial structure is useful to identify particular PAH degraders. However, the microbial community in the MSW composting soil and its change associated with prolonged exposure to PAHs and subsequent biodegradation remain largely unknown. In this study, anthracene was selected as a model compound. The bacterial community structure was investigated using terminal restriction fragment length polymorphism (TRFLP) and 16S rRNA gene clone library analysis. The two bimolecular tools revealed a large shift of bacterial community structure after anthracene amendment and subsequent biodegradation. Genera Methylophilus, Mesorhizobium, and Terrimonas had potential links to anthracene biodegradation, suggesting a consortium playing an active role.

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“…Using batch culture experiments, a relationship between bacterial community composition patterns and humic substance loading revealed that a DOC concentration above 0.54 mM favored tribes bacI-A1 and bacI-A2 of the phylum Bacteroidetes (51). Following a humic matter enrichment of lake water in another experiment, members of the Bacteroidetes related to Flexibacter (Chitinophaga) became the most abundant bacterial group, suggesting that they may play a significant role in humic matter degradation (104). The freshwater lake Bacteroidetes are often found in high abundance during periods following cyanobacterial blooms.…”

Section: Phylum Bacteroidetesmentioning

confidence: 99%

A Guide to the Natural History of Freshwater Lake Bacteria

Newton

Jones

Eiler

et al. 2011

Microbiol Mol Biol Rev

1,350

127

1,448

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SUMMARY Freshwater bacteria are at the hub of biogeochemical cycles and control water quality in lakes. Despite this, little is known about the identity and ecology of functionally significant lake bacteria. Molecular studies have identified many abundant lake bacteria, but there is a large variation in the taxonomic or phylogenetic breadths among the methods used for this exploration. Because of this, an inconsistent and overlapping naming structure has developed for freshwater bacteria, creating a significant obstacle to identifying coherent ecological traits among these groups. A discourse that unites the field is sorely needed. Here we present a new freshwater lake phylogeny constructed from all published 16S rRNA gene sequences from lake epilimnia and propose a unifying vocabulary to discuss freshwater taxa. With this new vocabulary in place, we review the current information on the ecology, ecophysiology, and distribution of lake bacteria and highlight newly identified phylotypes. In the second part of our review, we conduct meta-analyses on the compiled data, identifying distribution patterns for bacterial phylotypes among biomes and across environmental gradients in lakes. We conclude by emphasizing the role that this review can play in providing a coherent framework for future studies.

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Enrichment and cultivation of pelagic bacteria from a humic lake using phenol and humic matter additions

Cited by 18 publications

References 54 publications

Interspecific interactions drive chitin and cellulose degradation by aquatic microorganisms

Interspecific interactions drive chitin and cellulose degradation by aquatic microorganisms

Changes in bacterial community of anthracene bioremediation in municipal solid waste composting soil

A Guide to the Natural History of Freshwater Lake Bacteria

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