Enrichment and detection of small molecules using magnetic graphene as an adsorbent and a novel matrix of MALDI-TOF-MS

Abstract

Magnetic graphene composites were synthesized via a simple hydrothermal reaction, and the as-made composites were successfully applied as an adsorbent and a novel matrix in the enrichment and analysis of small molecules by matrix assisted laser desorption/ionization mass spectrometry.

Conventional Matrices Loaded Onto a Graphene Layer Enhances MALDI-TOF/TOF Signal: Its Application to Improve Detection of Phosphorylated Peptides

“…Since then, other articles have described the use of graphene as a matrix [1,[3][4][5], and the present work is the first study where graphene is used as a MALDI adjuvant improving ionization rate with a stabilizing effect that reduces the rate of fragmentation and gives rise to a better detection of phosphorylated peptides and a higher number of peaks detected in the analysis of complex samples. The enhancement of the signal with the combination of graphene/CHCA is especially useful in the case of complex samples where more peaks are detected, mainly in the high mass range of the spectra.…”

“…I n the last few years, the use of graphene as a new MALDI matrix for the analysis of low molecular weight molecules has been extensively described [1][2][3][4][5][6]. The main advantages of this matrix are related to (1) the absence of interfering peaks in the spectra at low mass range, usually visible when using traditional matrices [7], and (2) an attenuation of analyte fragmentation [8], which could reduce the complexity of the spectra at higher signal intensities.…”

Graphene and graphene oxide as nanomaterials for medicine and biology application

“…Sometimes, it is necessary to wash the graphene after separation, which is done by water or some other solvents [156,157]. To minimize the loss of graphene, magnetic graphene can be prepared by combining Fe 3 O 4 or cobalt particles with graphene sheets [91,156,158].…”

Nanomaterial and their applications in Environmental sample preparation techniques: A Review

“…Luo et al 64 immobilized graphene sheets onto silica-coated magnetic microspheres by simple adsorption and used as an extraction media for the enrichment of trace amount of six sulfonamide antibiotics in environmental water samples. Liu et al 65 .…”