Enrichment and immobilization of quinone-respiring bacteria in anaerobic granular sludge

Abstract

The capacity of an anaerobic granular sludge for serving as an immobilizing mechanism for quinone-respiring bacteria was evaluated. The inoculum was continuously fed with a basal medium containing the humic model compound, anthraquinone-2,6-disulfonate (AQDS), as a terminal electron acceptor. Complete reduction of AQDS was achieved by the granular sludge for a prolonged period in an anaerobic bioreactor provided with a mixture of volatile fatty acids as a substrate. Phylogenetic analysis revealed the enrichment and immobilization of AQDS-respiring bacteria appearing as dominant organisms in the microbial population of the AQDS-supplemented reactor, compared to a reactor control operated under methanogenic conditions. The consistent quinone-reducing capacity observed in the consortium indicates that it is feasible to apply quinone-reducing microorganisms in continuous bioreactors and this ability can potentially be important in wastewaters rich in humic substances. The quinone reducing activity could also be applied to accelerate the conversion of xenobiotics susceptible to reductive biotransformations such as azo dyes and polychlorinated compounds in continuous bioreactors.

Enhancing the electron transfer capacity and subsequent color removal in bioreactors by applying thermophilic anaerobic treatment and redox mediators

“…Interestingly, in the current continuous flow experiment at 30jC, AQDS at a concentration of 0.025 mM did not have any toxic effect towards methanogens, as the CH 4 productions between the reactors AQDS-free and AQDS-supplemented were similar. This is in agreement with Cervantes et al (2003) who observed a rapid methanogenic activity recovery even using an AQDS concentration that is about 500-times higher than the AQS concentration used in the present investigation.…”

On-Water Reactivity and Regioselectivity of Quinones in C–N Coupling with Amines: Experimental and Theoretical Study

“…Quinones also have relevance in industrial applications such as dyes, [5] and in the biodegradation of priority pollutants. [6] Among quinones, aminoquinone derivatives are remarkable, based on the potential biological applications they posses. The aminoquinone motif [7] is a component of the molecular framework of numerous natural products, such as kinamycins, [8] caulibugulones, [9] griffithazanone A, [10] cyclosmenospongine, [11] and others, and they have been used as reactive intermediates for the synthesis of many biologically relevant compounds.…”

Biodegradación de colorantes azo por Pleurotus ostreatus

“…La concentración de colorantes en el agua residual de las empresas textiles puede variar de 100 a 500 mg/L (Sponza & Işik, 2004). Los colorantes más utilizados son los de tipo azo, cuya característica principal es el enlace insaturado de dos moléculas de nitrógeno (-N=N-), que son poco biodegradables, pero se ha demostrado que se pueden establecer las condiciones para que sean degradados y en ciertos casos mineralizados (Field et al, 2000;Cervantes et al, 2001;Cervantes et al, 2003;González & Escamilla, 2008). Cabe mencionar que los efluentes de la industria textil se caracterizan por presentar fluctuaciones extremas en parámetros, tales como la Demanda Química de Oxígeno (DQO), la Demanda Bioquímica de Oxígeno (DBO), pH, color y salinidad.…”