Enrichment and isolation of endosulfan degrading and detoxifying bacteria

Kumar, Koel; Devi, Sivanesan Saravana; Krishnamurthi, Kannan; Kanade, Gajanan S.; Chakrabarti, Tapan

doi:10.1016/j.chemosphere.2006.12.076

Cited by 68 publications

(41 citation statements)

References 14 publications

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“…Endosulfan diol was identifi ed as the degradation intermediate. Two cultures identifi ed by 16S rRNA as Stenotrophomonas maltophilia and Rhodococcus erythropolis were found to be responsible for majority of the degradation by the mixed culture 8 .…”

Section: Use Of Technology At Actual Sitementioning

confidence: 99%

Biodegradation and bioremediation of pesticide in soil: concept, method and recent developments

Singh

2008

Indian J Microbiol

165

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Biodegradation is a natural process, where the degradation of a xenobiotic chemical or pesticide by an organism is primarily a strategy for their own survival. Most of these microbes work in natural environment but some modifi cations can be brought about to encourage the organisms to degrade the pesticide at a faster rate in a limited time frame. This capability of microbe is some times utilized as technology for removal of contaminant from actual site. Knowledge of physiology, biochemistry and genetics of the desired microbe may further enhance the microbial process to achieve bioremediation with precision and with limited or no scope for uncertainty and variability in microbe functioning. Gene encoding for enzyme has been identifi ed for several pesticides, which will provide a new inputs in understanding the microbial capability to degrade a pesticide and develop a super strain to achieve the desired result of bioremediation in a short time.

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Section: Use Of Technology At Actual Sitementioning

confidence: 99%

Biodegradation and bioremediation of pesticide in soil: concept, method and recent developments

Singh

2008

Indian J Microbiol

165

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“…Each strain of Acineto bacter, except A. lwoffii, also contained a small amount of 12:0 2-OH (Moss et al, 1988). Previous researchers have reported that endosulfan can be used as the sole sulfur source (Hussain et al, 2009;Kataoka and Takagi, 2013;Kumar et al, 2014) or the sole carbon source for microbial growth (Sutherland et al, 2000;Kumar et al, 2007;Castillo et al, 2011). In this study, the isolates were screened for their ability to utilize α-endosulfan as the sole sulfur source.…”

Section: Discussionmentioning

confidence: 99%

“…However, in this study, we demonstrated that our strains could degrade α-endosulfan much higher or similiar than of previously reported bacterial strains. S. maltophilia E4, Rhodococcus erythropolis E5 (Kumar et al, 2007), P. aeruginosa MN2B14 (Hussain et al, 2009) degraded 46% (15 days), 24% (15 days) and 95% (14 days) of α-endosulfan, respectively. In the case of the experiment done by Yu et al (2012), Stenotrophomonas.…”

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confidence: 99%

Isolation and Characterization of α-Endosulfan Degrading Bacteria from the Microflora of Cockroaches

Özdal

Ozdal

Alguri

2016

Polish Journal of Microbiology

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A b s t r a c tExtensive applications of organochlorine pesticides like endosulfan have led to the contamination of soil and environments. Five different bacteria were isolated from cockroaches living in pesticide contaminated environments. According to morphological, physiological, biochemical properties, and total cellular fatty acid profile by Fatty Acid Methyl Esters (FAMEs), the isolates were identified as Pseudomonas aeruginosa G1, Stenotrophomonas maltophilia G2, Bacillus atrophaeus G3, Citrobacter amolonaticus G4 and Acinetobacter lwoffii G5. This is the first study on the bacterial flora of Blatta orientalis evaluated for the biodegradation of α-endosulfan. After 10 days of incubation, the biodegradation yields obtained from P. aeruginosa G1, S. maltophilia G2, B. atrophaeus G3, C. amolonaticus G4 and A. lwoffii G5 were 88.5% , 85.5%, 64.4%, 56.7% and 80.2%, respectively. As a result, these bacterial strains may be utilized for biodegradation of endosulfan polluted soil and environments. 64developed resistance to pesticides. If this is true, we can isolate new and effective α-endosulfan degrading bacteria from cockroaches' microflora and these isolates can be used for the biological treatment of waters and soils polluted with endosulfan and other insecticides. Experimental Material and MethodsChemicals. α-endosulfan and reagents were purchased from Sigma-Aldrich (St Louis, MO, USA). All solvents used were of the highest analytical grade and were employed without further purification. The stock α-endosulfan solution was prepared in acetone and used for all the experiments. The chemical structure and the most important physical parameters of endosulfan are summarized in Fig. 1 O, 24 (Siddique et al., 2003). After 7 days, 5 ml of each culture was re-inoculated into new α-endosulfan-NSM medium and further incubated at 30°C for 7 days. This subculture was repeated under the same culture conditions, and then an aliquot (0.2 ml) from each culture was applied to solid α-endosulfan-NSM for isolation of single colonies.Identification of endosulfan degrading microorganisms. α-endosulfan degrading bacterial isolates were identified by various tests, such as pigment formation, Gram staining, nitrate reduction, catalase and oxidase tests, and starch hydrolysis. Biochemical reactions were conducted according to Benson (2001). Biochemical activities were determined according to the recommended scheme of Bergey's Manual of Determinative Bacteriology (Holt et al., 1994). Identification was confirmed by the fatty acid analysis for all bacterial isolates. Fatty acid methyl ester (FAME) profiles of each bacterial strain were identified by comparing the commercial databases (Tripticase Soy Broth Agar 40) with the MIS software package. The identity of bacterial strains was revealed by computer comparison of FAME profiles of the unknown test strains with those in the library. FAMEs were separated by gas chromatography (HP6890, Hewlett Packard, Palo Alto, CA, USA) with a fused-silica capillary column (25 m × 0.2 mm) with cr...

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“…Maximum biodegradation by these three selected efficient bacterial strains was observed at an initial pH of 8.0 and at an incubation temperature of 30 º C. Kwon, et al, (2002) reported that the endosulfan diol is the major metabolite as pH increases, while endosulfan sulfate is the major metabolite as pH decreases. Kumar, et al, (2007) studied the degradation of endosulfan by a mixed culture isolated from a pesticide-contaminated soil was studied in batch experiments. After two weeks of incubation, the mixed culture was able to degrade 73% and 81% of alpha and beta endosulfan respectively.…”

Section: Discussionmentioning

confidence: 99%

Effect of tween 80 added to the soil on the degradation of endosulfan by Pseudomonas aeruginosa

Jayashree

Vasudevan

2007

Int. J. Environ. Sci. Technol.

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Endosulfan, a chlorinated cyclodiene insecticide is of environmental concern because of its apparent persistence and toxicity to many non target organisms. Endosulfan is hydrophobic and persists in soil for more than a year. To overcome the problem of hydrophobic and limited availability, surfactants play a major role in soil remediation. In the present study, the effect of Tween 80 added to the soil on the degradation of endosulfan by Pseudomonas aeruginosa at different pH (7.0 and 8.5) was studied. The addition of synthetic surfactant Tween 80 enhanced the solubility and degradation of endosulfan. A significant degradation (94%) was observed in pH 8.5 and Tween 80 added soil; the bacterial population in the treatment unit T8 was 75 x 10 9 CFU/g of soil. The unit T4 inoculated at pH 8.5 showed 86 % alpha and 60 % beta endosulfan degradation, the bacterial population was 73 x 10 8 CFU / g of soil. The degradation of both the isomers were observed and accompanied with formation of endodiol and endosulfan sulfate.

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Enrichment and isolation of endosulfan degrading and detoxifying bacteria

Cited by 68 publications

References 14 publications

Biodegradation and bioremediation of pesticide in soil: concept, method and recent developments

Biodegradation and bioremediation of pesticide in soil: concept, method and recent developments

Isolation and Characterization of α-Endosulfan Degrading Bacteria from the Microflora of Cockroaches

Effect of tween 80 added to the soil on the degradation of endosulfan by Pseudomonas aeruginosa

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