Enrichment and Purification of Aucubin from Eucommia ulmoides Ionic Liquid Extract Using Macroporous Resins

Yang, Xinyu; Wei, Mian; Tian, Hao; Liu, Tingting; Yang, Lei

doi:10.3390/ma11091758

Cited by 12 publications

(9 citation statements)

References 26 publications

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“…The global HPLE-RP condition, 15%, 150 °C and 80%, allowed the highest recovery of phenolic acids, with gallic acid being the most abundant (~70%) (Table 4). Yang et al [62] observed a similar behavior during RP, arguing that probably the compounds of interest are more stabilized by the interactions with the solvent than by the interactions with the resin surface. Hence, the higher the ethanol content in the raw extracts, the lower the polyphenols content in the purified extracts (Table 4).…”

Section: Resultsmentioning

confidence: 89%

The Impact of Temperature and Ethanol Concentration on the Global Recovery of Specific Polyphenols in an Integrated HPLE/RP Process on Carménère Pomace Extracts

et al. 2019

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Sequential extraction and purification stages are required to obtain extracts rich in specific polyphenols. However, both separation processes are often optimized independently and the effect of the integrated process on the global recovery of polyphenols has not been fully elucidated yet. We assessed the impact of hot-pressurized liquid extraction (HPLE) conditions (temperature: 90–150 °C; ethanol concentration: 15%–50%) on the global recovery of specific phenolic acids, flavanols, flavonols and stilbenes from Carménère grape pomace in an integrated HPLE/resin purification (RP) process. HPLE of phenolic acids, flavanols and stilbenes were favored when temperature and ethanol concentration increased, except for chlorogenic acid which showed an increment of its Gibbs free energy of solvation at higher ethanol contents. Ethanol concentration significantly impacted the global yield of the integrated HPLE/RP process. The lower the ethanol content of the HPLE extracts, the higher the recovery of phenolic acids, flavanols and stilbenes after RP, except for flavonols which present more polar functional groups. The best specific recovery conditions were 150 °C and ethanol concentrations of 15%, 32.5% and 50% for phenolic acids, flavanols and stilbenes, and flavonols, respectively. At 150 °C and 32.5% of ethanol, the extracts presented the highest total polyphenol content and antioxidant capacity. The integrated HPLE/RP process allows a selective separation of specific polyphenols and eliminates the interfering compounds, ensuring the safety of the extracts at all evaluated conditions.

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Section: Resultsmentioning

confidence: 89%

The Impact of Temperature and Ethanol Concentration on the Global Recovery of Specific Polyphenols in an Integrated HPLE/RP Process on Carménère Pomace Extracts

et al. 2019

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“…In order to fully recover aucubin, it is necessary to select a suitable desorbent that can dissolve the resin-enriched aucubin as much as possible in a small volume of desorbent. Under the same conditions, the solubility of aucubin in ethanol is higher than that in water . Besides, ethanol can be recycled and reused.…”

Section: Resultsmentioning

confidence: 99%

“…Under the same conditions, the solubility of aucubin in ethanol is higher than that in water. 53 Besides, ethanol can be recycled and reused. On the basis of this, ethanol was selected as the desorbent in the desorption test.…”

Section: Adsorption Kineticsmentioning

confidence: 99%

Extraction and Purification of Aucubin from Eucommia ulmoides Seed Draff in Natural Deep Eutectic Solvents Using Macroporous Resins

Liao,

Chen,

Tang

et al. 2023

ACS Omega

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Aucubin (AU) is an active ingredient that exerts strong antioxidant and anti-inflammatory effects in the treatment of several diseases. In order to improve the efficiency of resource utilization of traditional biomass waste, Eucommia ulmoides seeddraff (EUSD) waste biomass was used as the raw material, and a series of deep eutectic solvents were selected to evaluate the extraction efficiency of aucubin from EUSD. A response surface experiment was designed based on a single-factor experiment to optimize the extract conditions. The results showed that the best conditions for aucubin extraction were an HBD−HBA ratio of 2.18, a liquid−solid ratio of 46.92 mL/g, a water percentage of 37.95%, a temperature of 321.03 K, and an extraction time of 59.55 min. The maximum amount of aucubin was 156.4 mg/g, which was consistent with the theoretical value (156.8 mg/g). Then, the performance of 12 resins for adsorption and desorption was contrasted. The results revealed that HPD950 resin exhibited the best performance, with an adsorption capacity of 95.2% and a desorption capacity of 94.3%. Additionally, the pseudo-second-order model provided the best match to the kinetics data, the Langmuir model provided the best fit to the isotherm data, and adsorption was a beneficial, spontaneous, exothermic, and physical process. In the recyclability test, the HPD950 resin had great potential and excellent sustainability in aucubin recovery. In the antioxidant activity study, the aucubin extract exerted a strong antioxidant ability with scavenging capabilities for four free radicals. Furthermore, the antifungal activity study found that the aucubin extract exhibited a good antifungal effect against 5 tested pathogens. The research results can provide a theoretical basis for the extraction of high-value components from waste biomass by deep eutectic solvent and a certain application value for the development and utilization of natural aucubin products.

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“…It is reported that chlorogenic acid can protect mice against Cd-induced hepatorenal injury via regulating intestinal flora balance ( Ding et al, 2021 ). Except chlorogenic acid, geniposidic acid and aucubin are the other characteristic components with high contents in E. ulmoides ( Wang et al, 2016 ; Yang et al, 2018 ). Based on the rich foundation of natural products, E. ulmoides possess multiple pharmacological activities, such as anti-inflammatory and antihyperlipidemia ( Wang et al, 2019 ; Gu et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation Eucommia ulmoides extract at high content served as a feed additive in the hens industry

et al. 2022

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Since numerous natural components in Eucommia ulmoides belong to phytoestrogen, its effect on hens production deserve more attention. To investigate the potential of E. ulmoides extract used as a feed additive, laying performance, egg quality, yolk cholesterol, yolk fatty acids, yolk fatty, yolk volatile components, albumen amino acids, plasma biochemical parameters, intestinal histology, and gut microbiota of hens (n = 120) were determined between basal diet ( A ) and dietary supplementation low ( B ), middle ( C ), and high ( D ) level E. ulmoides extract for 11 wk. When compared to A group, 2 percentage points elevation in laying rate was observed of D group. Significant up-regulation of immunoglobulin indexes and down-regulation of lipid related indexes in D group were also found if comparison with A group, suggesting that supplementation E. ulmoides extract at a relative high content benefited in immunity enhancing and blood-fat depressing. Meanwhile, obvious variation in albumen amino acids and yolk volatile compounds were inspected as dietary supplementation E. ulmoides extract, especially in D group, implied that the flavor of egg would change under high-level E. ulmoides extract treatment. Besides, villus height and villus height to crypt depth ratio of duodenum, jejunum, and ileum in D group were also significantly higher than that of in A group, indicating high-level E. ulmoides extract contributed to nutrient adsorption via intestinal histology changing. Moreover, the richness, diversity, and composition of gut microbiota in D group also significantly altered with a comparison of A group. These variation caused gut microbiota in D group major enriched in the KEGG pathway of insulin signing pathway, systemic lupus erythematosus, and bacterial invasion of epithelial cells, which were conducive to egg production elevation via facilitating nutrient adsorption, inflammation relieving, blood lipid amelioration, and insulin resistance alleviation. These results indicated that dietary supplementation E. ulmoides extract at high content could serve as a feed additive in the hens industry.

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Enrichment and Purification of Aucubin from Eucommia ulmoides Ionic Liquid Extract Using Macroporous Resins

Cited by 12 publications

References 26 publications

The Impact of Temperature and Ethanol Concentration on the Global Recovery of Specific Polyphenols in an Integrated HPLE/RP Process on Carménère Pomace Extracts

The Impact of Temperature and Ethanol Concentration on the Global Recovery of Specific Polyphenols in an Integrated HPLE/RP Process on Carménère Pomace Extracts

Extraction and Purification of Aucubin from Eucommia ulmoides Seed Draff in Natural Deep Eutectic Solvents Using Macroporous Resins

Dietary supplementation Eucommia ulmoides extract at high content served as a feed additive in the hens industry

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