Enrichment of activated sludge for enhanced hydrogen production from crude glycerol

Varrone, Cristiano; Rosa, Sílvia; Fiocchetti, Floriana; Giussani, Barbara; Izzo, G.; Massini, Giulia; Marone, Antonella; Signorini, Antonella; Wang, Aijie

doi:10.1016/j.ijhydene.2012.11.069

Cited by 47 publications

(31 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This fermentation has been improved by modifying the microorganisms, the culture media, or the reactor conditions. The bacteria applied normally came from methanogenic fermentation including several types of hydrogenic bacteria (Rossi et al 2011); however, the strains have been improved using molecular techniques (transgenic strains) (Gonzalez et al 2008) or selection techniques (eco-biotechnological) (Varrone et al 2013b), and these methodologies have allowed the increment of H 2 generation and glycerol consumption. In dark fermentation, culture medium has achieved improvements in the hydrogen production.…”

Section: Microbial Fuel Cellsmentioning

confidence: 99%

Conversion of residues and by-products from the biodiesel industry into value-added products

Plácido

Capareda

2016

Bioresour. Bioprocess.

View full text Add to dashboard Cite

Biodiesel, one of the most important sources of renewable energy, is produced in large quantities around the world; however, its production generates different kinds of residues and by-products which raise economic and environmental concerns. This review presents a compilation of the data on current state of transformation of residues and by-products of biodiesel industry into products that are suitable for bio-refining. The review has analyzed glycerol, biodiesel washing wastewaters, and solid residues. The technologies were described and the most significant experimental results and variables were summarized to allow researchers an easy access to this information.

show abstract

Section: Microbial Fuel Cellsmentioning

confidence: 99%

Conversion of residues and by-products from the biodiesel industry into value-added products

Plácido

Capareda

2016

Bioresour. Bioprocess.

View full text Add to dashboard Cite

show abstract

“…Therefore, crude glycerol production exceeds the current commercial demand of purified glycerol since the last decade and has been recently proposed as a carbon source for bioenergy production (Singhabhandhu and Tezuka 2010). It is a potential promising feedstock for hydrogen production, since it is a simple substrate that is easily biodegradable during glycolysis by an oxidative pathway (Siles et al 2010;Varrone et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…When mixed cultures coming from anaerobic digester were used in continuous systems with low HRT, low hydrogen yields were reported (0.05 mol H2 mol −1 glycerol ), but higher yields were observed after inoculum pretreatment through thermal shock (0.11-0.41 mol H2 mol −1 glycerol ) (Temudo et al 2008). However, this type of pretreatment is unsuitable at industrial scale because it implies an extra energetic cost and a lack of robustness of the biomass to face operational failures (Ren et al 2008;Akutsu et al 2009;Seifert et al 2009;Varrone et al 2013). Therefore, it is interesting to study a glycerol adaptation strategy of the mixed culture without heat-pretreatment of the inoculum.…”

Section: Introductionmentioning

confidence: 99%

Adaptation of acidogenic sludge to increasing glycerol concentrations for biohydrogen production

Tapia-Venegas

Cabrol

Brandhoff

et al. 2015

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Hydrogen is a promising alternative as an energetic carrier and its production by dark fermentation from wastewater has been recently proposed, with special attention to crude glycerol as potential substrate. In this study, two different feeding strategies were evaluated for replacing the glucose substrate by glycerol substrate: a one-step strategy (glucose was replaced abruptly by glycerol) and a step-by-step strategy (progressive decrease of glucose concentration and increase of glycerol concentration from 0 to 5 g L(-1)), in a continuous stirred tank reactor (12 h of hydraulic retention time (HRT), pH 5.5, 35 °C). While the one-step strategy led to biomass washout and unsuccessful H2 production, the step-by-step strategy was efficient for biomass adaptation, reaching acceptable hydrogen yields (0.4 ± 0.1 molH2 mol(-1) glycerol consumed) around 33 % of the theoretical yield independently of the glycerol concentration. Microbial community structure was investigated by single-strand conformation polymorphism (SSCP) and denaturing gradient gel electrophoresis (DGGE) fingerprinting techniques, targeting either the total community (16S ribosomal RNA (rRNA) gene) or the functional Clostridium population involved in H2 production (hydA gene), as well as by 454 pyrosequencing of the total community. Multivariate analysis of fingerprinting and pyrosequencing results revealed the influence of the feeding strategy on the bacterial community structure and suggested the progressive structural adaptation of the community to increasing glycerol concentrations, through the emergence and selection of specific species, highly correlated to environmental parameters. Particularly, this work highlighted an interesting shift of dominant community members (putatively responsible of hydrogen production in the continuous stirred tank reactor (CSTR)) according to the gradient of glycerol proportion in the feed, from the family Veillonellaceae to the genera Prevotella and Clostridium sp., putatively responsible of hydrogen production in the CSTR.

show abstract

“…Studies for the enhancement of the yield and the production rate have evaluated the effect of pH, temperature, bioreactor design, and substrates [3][4][5], as well as the use of inocula made of pure cultures or artificial consortia [6,7]. In order to increase the sustainability of the process, experiments have been conducted using wastewater or organic wastes as substrates [8,9]. Slurry and other environmental matrices have also been selected and used as inocula since they are rich in H 2 producers [3,4,[7][8][9].…”

Section: Introductionmentioning

confidence: 99%

“…In order to increase the sustainability of the process, experiments have been conducted using wastewater or organic wastes as substrates [8,9]. Slurry and other environmental matrices have also been selected and used as inocula since they are rich in H 2 producers [3,4,[7][8][9]. The members of bacterial communities during H 2 production were mainly identified as facultative anaerobes, belonging to the Enterobacteriaceae and several species of the strict anaerobe Clostridium [1].…”

Section: Introductionmentioning

confidence: 99%

Characterization by length heterogeneity (LH)-PCR of a hydrogen-producing community obtained in dark fermentation using coastal lake sediment as an inoculum

et al. 2013

Self Cite

View full text Add to dashboard Cite

Background: Sustainable biohydrogen production can be achieved by dark fermentation of organic wastes, and studies were carried out using a range of substrates and inocula. The bacterial populations involved were mainly identified as facultative anaerobes (Enterobacteriaceae) and strictly anaerobes (Clostridiales), and their dynamics in relation to H 2 and metabolite production depends on the physical and environmental conditions of the bioreactor. This study has evaluated the use of length heterogeneity (LH)-PCR fingerprinting to detect changes in the microbial community during continuous hydrogen production under dark fermentation.

show abstract

Enrichment of activated sludge for enhanced hydrogen production from crude glycerol

Cited by 47 publications

References 52 publications

Conversion of residues and by-products from the biodiesel industry into value-added products

Conversion of residues and by-products from the biodiesel industry into value-added products

Adaptation of acidogenic sludge to increasing glycerol concentrations for biohydrogen production

Characterization by length heterogeneity (LH)-PCR of a hydrogen-producing community obtained in dark fermentation using coastal lake sediment as an inoculum

Contact Info

Product

Resources

About