Enrichment of diterpenes in green coffee oil using supercritical fluid extraction – Characterization and comparison with green coffee oil from pressing

Oliveira, Paola Maressa Aparecida de; Almeida, Rafael Henrique de; Oliveira, Naila Albertina de; Bostyn, Stéphane; Gonçalves, Cintia B.; Oliveira, Alessandra Lopes de

doi:10.1016/j.supflu.2014.08.016

Cited by 58 publications

(38 citation statements)

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“…7 According to literature reports, coffee oil could also be used as raw materials for the production of cosmetics as both linoleic acid and palmitic acid were effective in mitigating skin inflammation and skin aging. 4 For instance, Velazquez Pereda et al 8 reported that following treatment with green coffee oil (3.12-25.0 mg/mL), both human derma fibroblast cell CC-2511 and human skin keratin cell HaCaT could induce the formation of collagen, elastin, and glycosaminoglycans by 1.8-, 1.5-, and 2-fold, respectively. In another study dealing with the preparation of emulsion with coffee oil as raw materials, Wagemaker et al 9 illustrated that coffee oil was efficient in treating skin damage caused by ultraviolet B (UVB; 280-320 nm) radiation, with the sun protection factor (SPF) being 1.5.…”

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confidence: 99%

“…19,20 However, both Ferrari et al 21 and de Oliveira et al 4 pointed out that coffee oil obtained from green coffee bean was composed of about 75% triacylglycerol, a small amount of free fatty acid, and unsaponifiables including sterol, tocopherol, phospholipid, and diterpene. This outcome indicated that coffee oil recovered from coffee grounds or green coffee bean possessed a similar composition.…”

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Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

Yang¹,

Hung²,

Chen³

2017

IJN

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Coffee grounds, a waste by-product generated after making coffee, contains approximately 15% coffee oil which can be used as a raw material in cosmetics. Algae oil rich in docosahexaenoic acid (DHA) has been demonstrated to possess anticancer and anti-inflammation functions. The objectives of this study were to develop a gas chromatography-mass spectrometry (GC-MS) method for the determination of fatty acids in coffee oil and algae oil and prepare a nanoemulsion for studying its inhibition effect on ultraviolet A-induced skin damage in mice and growth of melanoma cells B16-F10. A total of 8 and 5 fatty acids were separated and quantified in coffee oil and algae oil by GC-MS, respectively, with linoleic acid (39.8%) dominating in the former and DHA (33.9%) in the latter. A nanoemulsion with a particle size of 30 nm, zeta potential −72.72 mV, and DHA encapsulation efficiency 100% was prepared by using coffee oil, algae oil, surfactant (20% Span 80 and 80% Tween 80), and deionized water. Differential scanning calorimetry (DSC) analysis revealed a high stability of nanoemulsion when heated up to 110°C at a pH 6, whereas no significant changes in particle size distribution and pH occurred over a 90-day storage period at 4°C. Animal experiments showed that a dose of 0.1% coffee oil-algae oil nanoemulsion was effective in mitigating trans-epidermal water loss, skin erythema, melanin formation, and subcutaneous blood flow. Cytotoxicity test implied effective inhibition of melanoma cell growth by nanoemulsion with an IC 50 value of 26.5 µg/mL and the cell cycle arrested at G2/M phase. A dose-dependent upregulation of p53, p21, cyclin B, and cyclin A expressions and downregulation of CDK1 and CDK2 occurred. Also, both Bax and cytochrome c expressions were upregulated and bcl-2 expression downregulated, accompanied by a rise in caspase-3, caspase-8, and caspase-9 activities for apoptosis execution. Collectively, the apoptosis pathway of melanoma cells B16-F10 may involve both mitochondria and death receptor.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

Yang¹,

Hung²,

Chen³

2017

IJN

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“…Juntamente com os fenômenos de absorção, distribuição e excreção, a biotransformação também participa da regulação de níveis plasmáticos de drogas e substâncias em geral. A quantificação dos bioativos cafestol e caveol do óleo de café verde extraído via CO 2 supercrítico mostrou concentrações semelhantes ao estudo De Oliveira et al (2014) corroborando com o fato de que a extração supercrítica tem uma indicação positiva no desenvolvimento de fitoterápicos a partir de matrizes vegetais.…”

Section: Bioquímica E Hemograma (Toxicidade De Doses Repetidas)unclassified

“…Antecedendo a extração dinâmica, os grãos de café verde triturados permaneceram em contado com o CO 2 supercrítico durante o tempo estático de 20 min. O tempo total de extração dinâmica com CO 2 supercrítico foi de 6 h. A massa de grãos de café verde triturado utilizada em todas as 40 extrações foi de aproximadamente 88,6g, como também estabelecido porDe Oliveira et al (2014). Brasil é um país com potencial como produtor deste solvente.…”

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Análise da concentração de compostos bioativos e avaliação da toxicidade aguda in vivo dos diterpenos cafestol e caveol presentes no óleo de grãos de café verdes obtidos por extração supercrítica e por extração com fluido pressurizado

Oliveira¹

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OLIVEIRA, N. A. Concentration of bioactive compounds analysis and evaluation of acute toxicity in vivo of the diterpenes cafestol and kahweol from green beans coffee oil obtained by supercritical and pressurized fluid extractions. 2015. 111 f. M. Sc. Dissertation -Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo, Pirassununga, 2015.This study aimed to evaluate the bioactivity of the diterpenes in green coffee oil, cafestol (C), and kahweol (K) using supercritical fluid extraction (SFE-CO 2 ), and to optimize the extraction with pressurized liquid extraction (PLE) in batch by using a rotatable central composite design (RCCD 2 ). This issue has innovative and novel character, since the pressurized liquid extraction technology available until now for analyte extraction has been studied in vegetable oil extraction processes such as Pequi oil, green coffee oil, and Pitanga seeds extracts, at LTAPPN. This technique uses organic solvents and high extraction temperature, which increases the solvent solubilization capacity. The use of high pressure accelerates the diffusion rates into the pores of the matrix, due to the lower solvent viscosity. This behavior provides greater penetration of the solvent into the matrix, increasing solvent extraction capacity. The supercritical fluid extraction (SFE) is a clean technology as it does not employ organic solvents, besides being a promising alternative to obtain extracts enriched with bioactive compounds. The results of the acute activity of diterpenes in green coffee oil extracted by SFE and PLE showed that although no lethality was observed in the animals using the oil extracted by SFE at a dose of 2,000 mg / kg in an acute toxicity study, and 25, 50, and 75 mg / kg in a repeated dose toxicity study, changes were observed in biochemical, hematological, and histological parameters. However, more in vivo studies about the effects of green coffee oil are required for pharmaceutical, food, and cosmetic applications. Similarly, the technological innovation to obtain the green coffee oil aims at obtaining extracts enriched in diterpenes, preventing degradation and increasing stability. The results indicate that green coffee oil extracted by SFE and PLE (in batch) have high concentrations of the active compounds cafestol and kahweol, and the best extraction condition was the trial 4 (70 ° C, and 8 min), and the highest oil yield was 9.78%

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Novel Extraction Techniques for Bioactive Compounds from Herbs and Spices

Sánchez‐Camargo

Montero

Mendiola

et al. 2020

Herbs, Spices and Medicinal Plants

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Enrichment of diterpenes in green coffee oil using supercritical fluid extraction – Characterization and comparison with green coffee oil from pressing

Cited by 58 publications

References 22 publications

Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

Preparation of coffee oil-algae oil-based nanoemulsions and the study of their inhibition effect on UVA-induced skin damage in mice and melanoma cell growth

Análise da concentração de compostos bioativos e avaliação da toxicidade aguda in vivo dos diterpenos cafestol e caveol presentes no óleo de grãos de café verdes obtidos por extração supercrítica e por extração com fluido pressurizado

Novel Extraction Techniques for Bioactive Compounds from Herbs and Spices

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