Enrichment of live unlabelled cardiomyocytes from heterogeneous cell populations using manipulation of cell settling velocity by magnetic field

Sofla, Aarash; Cirkovic, B.; Hsieh, Anne; Miklas, Jason W.; Filipović, Nenad; Radišić, Milica

doi:10.1063/1.4791649

Cited by 20 publications

(22 citation statements)

References 37 publications

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“…A device consisting of a column wrapped around with Ni wire placed between magnets sorted live cardiomyocytes from a heterogeneous solution in a label-free microfluidic approach with 93% purity. 10 Osman et al separated Jurkat cells labeled with magnetic nanoparticles from a solution spiked with human embryonic kidney under a continuous flow utilizing an integrated flat micro-patterned hard magnetic film.…”

Section: B)mentioning

confidence: 99%

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

et al. 2014

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This study describes the development and testing of a magnetic microfluidic chip (MMC) for trapping and isolating cells tagged with superparamagnetic beads (SPBs) in a microfluidic environment for selective treatment and analysis. The trapping and isolation are done in two separate steps; first, the trapping of the tagged cells in a main channel is achieved by soft ferromagnetic disks and second, the transportation of the cells into side chambers for isolation is executed by tapered conductive paths made of Gold (Au). Numerical simulations were performed to analyze the magnetic flux and force distributions of the disks and conducting paths, for trapping and transporting SPBs. The MMC was fabricated using standard microfabrication processes. Experiments were performed with E. coli (K12 strand) tagged with 2.8 μm SPBs. The results showed that E. coli can be separated from a sample solution by trapping them at the disk sites, and then isolated into chambers by transporting them along the tapered conducting paths. Once the E. coli was trapped inside the side chambers, two selective treatments were performed. In one chamber, a solution with minimal nutrition content was added and, in another chamber, a solution with essential nutrition was added. The results showed that the growth of bacteria cultured in the second chamber containing nutrient was significantly higher, demonstrating that the E. coli was not affected by the magnetically driven transportation and the feasibility of performing different treatments on selectively isolated cells on a single microfluidic platform.

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Section: B)mentioning

confidence: 99%

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

et al. 2014

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“…Techniques that rely upon magnetic forces to manipulate cells are not limited to those that use particles as the magnetic source (Sofla et al, 2013). The intrinsic magnetic properties of select cells allow for label-free manipulation without the potential interference of attached particles.…”

Section: Overview Of Cell Separation Methodsmentioning

confidence: 99%

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

2014

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Magnetic sorting using magnetic beads has become a routine methodology for the separation of key cell populations from biological suspensions. Due to the inherent ability of magnets to provide forces at a distance, magnetic cell manipulation is now a standardized process step in numerous processes in tissue engineering, medicine, and in fundamental biological research. Herein we review the current status of magnetic particles to enable isolation and separation of cells, with a strong focus on the fundamental governing physical phenomena, properties and syntheses of magnetic particles and on current applications of magnet-based cell separation in laboratory and clinical settings. We highlight the contribution of cell separation to biomedical research and medicine and detail modern cell separation methods (both magnetic and non-magnetic). In addition to a review of the current state-of-the-art in magnet-based cell sorting, we discuss current challenges and available opportunities for further research, development and commercialization of magnetic particle-based cell separation systems.

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“…The use of microfluidic chips has been also demonstrated to measure the bladder cancer biomarker APOA1 in human urine 26 and to enrich live cardiomyocytes from adult mouse hearts. 27 Donolato et al 28 have recently described an on-chip magnetic particle conveyor for transport and separation of magnetic particles.…”

Section: Introductionmentioning

confidence: 99%

On-chip magnetophoretic isolation of CD4 + T cells from blood

Darabi

Guo

2013

Biomicrofluidics

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This paper presents the design, fabrication, and testing of a magnetophoretic bioseparation chip for the rapid isolation and concentration of CD4 þ T cells from the peripheral blood. In a departure from conventional magnetic separation techniques, this microfluidic-based bioseperation device has several unique features, including locally engineered magnetic field gradients and a continuous flow with a buffer switching scheme to improve the performance of the separation process. Additionally, the chip is capable of processing significantly smaller sample volumes than conventional methods and sample losses are eliminated due to decreased handling. Furthermore, the possibility of sample-to-sample contamination is reduced with the disposable format. The overall dimensions of the device were 22 mm by 60 mm by 1 mm, approximately the size of a standard microscope slide. The results indicate a cell purity of greater than 95% at a sample flow rate of 50 ml/h and a cell recovery of 81% at a sample flow rate of 10 ml/h. The cell purity was found to increase with increasing the sample flow rate. However, the cell recovery decreases with an increase in the flow rate. A parametric study was also performed to investigate the effects of channel height, substrate thickness, magnetic bead size, and number of beads per cell on the cell separation performance. V C 2013 AIP Publishing LLC. [http://dx

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Enrichment of live unlabelled cardiomyocytes from heterogeneous cell populations using manipulation of cell settling velocity by magnetic field

Cited by 20 publications

References 37 publications

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

Isolation of cells for selective treatment and analysis using a magnetic microfluidic chip

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review

On-chip magnetophoretic isolation of CD4 + T cells from blood

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