Enrichment of Malaria Parasites by Antibody Immobilized Magnetic Nanoparticles

Tangchaikeeree, Tienrat; Jangpatarapongsa, Kulachart; Polpanich, Duangporn; Thiramanas, Raweewan; Pornjarone, Atcharavalai; Udnaen, Somkiat; Udomsangpetch, Rachanee; Tangboriboonrat, Pramuan

doi:10.1166/jbn.2013.1669

Cited by 12 publications

(7 citation statements)

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“…Preferential selection of fast-growing parasite haplotypes would preclude characterization of parasite variants that grow poorly in culture or those that are outcompeted during culture. Parasite enrichment using culture-free methods ( Trang et al, 2004 ; Tangchaikeeree et al, 2013 ) may be required to minimize potential bias in resolving within-host parasite ecology of low-density infections. Recent improvements in targeted capture of parasite-infected erythrocytes from complex mixtures and single cell sequencing ( Trevino et al, 2017 ), coupled with repeat blood sampling should aid the resolution of within-host ecology of asymptomatic infections at unprecedented depth and scale.…”

Section: Discussionmentioning

confidence: 99%

Intra-host dynamics of co-infecting parasite genotypes in asymptomatic malaria patients

Nkhoma

Banda

Khoswe

et al. 2018

Infection, Genetics and Evolution

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Malaria-infected individuals often harbor mixtures of genetically distinct parasite genotypes. We studied intra-host dynamics of parasite genotypes co-infecting asymptomatic adults in an area of intense malaria transmission in Chikhwawa, Malawi. Serial blood samples (5 ml) were collected over seven consecutive days from 25 adults with asymptomatic Plasmodium falciparum malaria and analyzed to determine whether a single peripheral blood sample accurately captures within-host parasite diversity. Blood samples from three of the participants were also analyzed by limiting dilution cloning and SNP genotyping of the parasite clones isolated to examine both the number and relatedness of co-infecting parasite haplotypes. We observed rapid turnover of co-infecting parasite genotypes in 88% of the individuals sampled (n = 22) such that the genetic composition of parasites infecting these individuals changed dramatically over the course of seven days of follow up. Nineteen of the 25 individuals sampled (76%) carried multiple parasite genotypes at baseline. Analysis of serial blood samples from three of the individuals revealed that they harbored 6, 12 and 17 distinct parasite haplotypes respectively. Approximately 70% of parasite haplotypes recovered from the three extensively sampled individuals were unrelated (proportion of shared alleles <83.3%) and were deemed to have primarily arisen from superinfection (inoculation of unrelated parasite haplotypes through multiple mosquito bites). The rest were related at the half-sib level or greater and were deemed to have been inoculated into individual human hosts via parasite co-transmission from single mosquito bites. These findings add further to the growing weight of evidence indicating that a single blood sample poorly captures within-host parasite diversity and underscore the importance of repeated blood sampling to accurately capture within-host parasite ecology. Our data also demonstrate a more pronounced role for parasite co-transmission in generating within-host parasite diversity in high transmission settings than previously assumed. Taken together, these findings have important implications for understanding the evolution of drug resistance, malaria transmission, parasite virulence, allocation of gametocyte sex ratios and acquisition of malaria immunity.

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Section: Discussionmentioning

confidence: 99%

Intra-host dynamics of co-infecting parasite genotypes in asymptomatic malaria patients

Nkhoma

Banda

Khoswe

et al. 2018

Infection, Genetics and Evolution

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“…Our result is in line with previous reports. 25,38 The reactive epoxy-functionalized mPDGs were easily bioconjugated with the anti-human Vd2 antibody and the covalent binding process leads to a strong conjugation which is suitable for various types of biologic application. Moreover, the binding efficiency can be further elevated by varying the amount of antibodies or the incubation time of the interaction.…”

Section: Characterizations Of Mpdgsmentioning

confidence: 99%

Enrichment of human Vγ9Vδ2 T lymphocytes by magnetic poly(divinylbenzene-co-glycidyl methacrylate) colloidal particles conjugated with specific antibody

et al. 2018

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“…This strategy has been used for the separation/concentration of viruses, bacteria, parasites, protein biomarkers, nucleic acids, antibiotics, and toxins, and for bioremediation (pesticides removal) purposes. − Considering the availability and stability of ligands and the simplicity of the protocols, functionalized MPs will most likely have an increased role in sample preparation procedures.…”

Section: Sample Preparation Considerationsmentioning

confidence: 99%

Lab-on-Chip Devices: Gaining Ground Losing Size

Romao¹,

Martins²,

Germano³

et al. 2017

ACS Nano

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Portable analytical devices are notably gaining relevance in the panorama of urgent testing. Such devices have the potential to play an important role as easy-to-handle tools in critical situations. Epidemic infectious disease agents (e.g., Ebola virus, Coronavirus, Zika virus) could be controlled more easily by testing travelers on-site at the country borders to prevent outbreaks from spreading. The increasing incidence of hospital-acquired infections caused by antibiotic resistant pathogens could be minimized by point-of-care microbial analysis as well as rapid screening tests of bacteria resistance. The threat of bioterrorism using novel unknown bioweapons has never been so high, thus, in-the-field early identification of the biological agent is crucial for triggering a coordinated response. Food allergies are a growing public health concern-allergic reactions can result in anaphylactic shock, which can prove fatal in minutes-thus, the ability to test foods for common allergens, rapidly and locally, before ingestion, would improve food safety for those with allergies. Lab-on-chip devices are becoming widely available for diverse applications and are becoming increasingly affordable. However, to shrink in price and size simultaneously, some trade-offs must be made. In this Perspective, we present considerations about product specifications, design concepts, and application scenarios.

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Enrichment of Malaria Parasites by Antibody Immobilized Magnetic Nanoparticles

Cited by 12 publications

References 0 publications

Intra-host dynamics of co-infecting parasite genotypes in asymptomatic malaria patients

Intra-host dynamics of co-infecting parasite genotypes in asymptomatic malaria patients

Enrichment of human Vγ9Vδ2 T lymphocytes by magnetic poly(divinylbenzene-co-glycidyl methacrylate) colloidal particles conjugated with specific antibody

Lab-on-Chip Devices: Gaining Ground Losing Size

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