Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing

Chen, Szu-Ying; Huang, Po-Hsien

doi:10.3791/65352

JoVE

2023

DOI: 10.3791/65352

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Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing

Szu-Ying Chen

Po-Hsien Huang

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2024

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Transcriptome-wide RNA 5-methylcytosine profiles of human iPSCs and iPSC-derived cardiomyocytes

Chen,

Fang,

Huang

et al. 2024

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Cardiac regenerative therapy has recently progressed by reprogramming somatic cells into induced pluripotent stem cells (iPSCs) and advanced by large-scale differentiation-derived cardiomyocytes (hiPSC-CMs). However, repairing damaged cardiac tissues with hiPSC-CMs remains limited due to immune rejection, cardiac arrhythmias, and concerns over tumor formation after hiPSC-CM transplantation. Despite efforts in profiling epigenomic changes during cardiac differentiation, regulatory mechanisms underlying 5-methylcytosine (m 5 C) deposition in RNA m 5 C epitranscriptomic landscape during hiPSC-to-cardiomyocyte differentiation remain unclear. Herein, bisulfite RNA-sequencing analysis was conducted in human pluripotent stem cells (hPSCs) from three independent cellular origins, and their derived cardiomyocytes (hPSC-CM), metabolic-maturation of derived cardiomyocytes (hPSC-CM-lac) and biochemical-enhanced derived cardiomyocytes (hPSC-CM-TDI). Integrated analysis of differentially methylated RNA m 5 C profiles and transcriptome-wide expression facilitated the identification of m 5 C sites coupled to the cardiomyocyte differentiation and RNA-dependent regulatory mechanisms of stem cell pluripotency. The RNA m 5 C profiles in this dataset allow the evaluations of the m 5 C level and distribution of specific m 5 C loci and facilitate understanding of the m 5 C epitranscriptomic landscape in biological functions of hPSC-CM beyond in vivo transplantation barriers.

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Transcriptome-wide RNA 5-methylcytosine profiles of human iPSCs and iPSC-derived cardiomyocytes

Chen,

Fang,

Huang

et al. 2024

Sci Data

View full text Add to dashboard Cite

show abstract

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Enrichment of mRNA and Bisulfite-mRNA Library Preparation for Next-Generation Sequencing

Cited by 1 publication

References 0 publications

Transcriptome-wide RNA 5-methylcytosine profiles of human iPSCs and iPSC-derived cardiomyocytes

Transcriptome-wide RNA 5-methylcytosine profiles of human iPSCs and iPSC-derived cardiomyocytes

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