Enrichment of nitrite-dependent anaerobic methane oxidizing bacteria in a membrane bioreactor

Allegue, Tomás; Arias, Adrián; Fernandez‐Gonzalez, Nuria; Omil, F.; Garrido, Juan Manuel Méndez

doi:10.1016/j.cej.2018.04.134

Cited by 78 publications

(35 citation statements)

References 38 publications

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“…In this study, only batch incubations fed with nitrite showed N 2 O production, while no N 2 O production was detected in incubations with only nitrate as an electron acceptor. In other studies, similar effects on N 2 O production by cultures after adding pulses of nitrite have been observed (Allegue et al 2018; Itokawa et al 2001). This might explain the observations in batch incubations, where the microbial community is subjected to pulse feeding of substrates.…”

Section: Discussionsupporting

confidence: 76%

“…Methanoperedens-like archaea at a rate of 57 mg N L −1 day −1 and the total nitrogen removal efficiency of the culture was as high as 97.5%. The rates for ammonium and nitrite removal reported here are amongst the higher rates for anammox/N-damo cultures (Allegue et al 2018; Liu et al 2019; Luesken et al 2011a, 2011b; Shi et al 2013; Xie et al 2017a, b). Yet, as in these previous studies, it was shown that in a mixed anammox/N-damo culture, the presence of Ca.…”

Section: Discussionmentioning

confidence: 47%

“…As a proof of principle, we show that an active nitrogen/methane-removing community can be established, but we realize that for applications the supply will be through the water. Previous studies used methane supply via a membrane system (Allegue et al 2018; Chen et al 2015; Wu and Zhang 2017). These systems may be good alternatives if biofilm growth on the membranes is carefully controlled.…”

Section: Discussionmentioning

confidence: 99%

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Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Stultiens

Guerrero-Cruz

Kessel

et al. 2019

Appl Microbiol Biotechnol

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The reject water of anaerobic digestors still contains high levels of methane and ammonium that need to be treated before these effluents can be discharged to surface waters. Simultaneous anaerobic methane and ammonium oxidation performed by nitrate/nitrite-dependent anaerobic methane-oxidizing(N-damo) microorganisms and anaerobic ammonium-oxidizing(anammox) bacteria is considered a potential solution to this challenge. Here, a stable coculture of N-damo archaea, N-damo bacteria, and anammox bacteria was obtained in a sequencing batch reactor fed with methane, ammonium, and nitrite. Nitrite and ammonium removal rates of up to 455 mg N-NO 2 − L −1 day −1 and 228 mg N-NH 4 + L −1 were reached. All nitrate produced by anammox bacteria (57 mg N-NO 3 − L −1 day −1 ) was consumed, leading to a nitrogen removal efficiency of 97.5%. In the nitrite and ammonium limited state, N-damo and anammox bacteria each constituted about 30–40% of the culture and were separated as granules and flocs in later stages of the reactor operation. The N-damo archaea increased up to 20% and mainly resided in the granular biomass with their N-damo bacterial counterparts. About 70% of the nitrite in the reactor was removed via the anammox process, and batch assays confirmed that anammox activity in the reactor was close to its maximal potential activity. In contrast, activity of N-damo bacteria was much higher in batch, indicating that these bacteria were performing suboptimally in the sequencing batch reactor, and would probably be outcompeted by anammox bacteria if ammonium was supplied in excess. Together these results indicate that the combination of N-damo and anammox can be implemented for the removal of methane at the expense of nitrite and nitrate in future wastewater treatment systems.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 47%

Section: Discussionmentioning

confidence: 99%

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Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Stultiens

Guerrero-Cruz

Kessel

et al. 2019

Appl Microbiol Biotechnol

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“…The greater MLVSS value, the better the pellets would perform. The optimal time for nursery is seen from the highest MLVSS value, because the highest MLVSS value indicated the peak of the growth of microorganisms [16]. At that time, the active solution of ammonia oxidizers was ready for use and then immobilized into the pellets.…”

Section: A Microorganisms Growth In Active Suspensionmentioning

confidence: 99%

Development of Active Solids Activator (Pellet) Using Local Culture from Badung River, Bali to Enhance Nitrification Process of Goat Wastewater

Suyasa¹,

Sudiartha²

2021

International Journal on Advanced Science, Engineering and Information Technology

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Goat urine wastewater that is disposed of without being processed will certainly cause environmental pollution. Therefore it is necessary to process the livestock waste. Processing goat urine into fertilizer needs to be done by converting ammonia to nitrate, or what is called the nitrification process. Nitrification takes place in two stages of oxidation, the first is the oxidation of ammonium to nitrite, and the second is the oxidation of nitrite to nitrate with the help of oxygen. The materials used in this study were (NH4)2SO4,

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“…The relative abundance of Methanosaeta on N4M3 was 40.5% higher than the control biofilm. In some recent studies, Methanosaeta is found as the dominant methanogen in anaerobic reactors with nitrite, where methanogenesis and denitrification were simultaneously occurring (Allegue et al 2018). This suggesting that Methanosaeta has some tolerance to nitrite, leading to its increased abundance in N4M3.…”

Section: Biofilm Development On Nitrite Admixed Concrete In Wastewatermentioning

confidence: 95%

Understanding and controlling of concrete corrosion in sewers

Li¹

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Sulfide-induced concrete corrosion is the main cause of the deterioration and early structure failure of concrete sewers. Sewer corrosion poses potential issues of odor emission and public safety, and the cost of preventive measures is a significant economic problem globally. Our understanding of sewer corrosion has increased dramatically in recent years. However, there is limited knowledge of the concrete corrosion at high H2S levels and the effective corrosion mitigation strategies. The overall aim of this thesis is to improve the understanding of the sewer corrosion processes and develop a long-lasting corrosion mitigation strategy. To improve the understanding of sewer corrosion, the corrosion development on concrete coupons was investigated in sewers with high H2S concentrations. In a pilot sewer with gaseous H2S at 1100±100 ppm, the surface pH of concrete was reduced from 10.5±0.3 to 3.1±0.5 within 20 days and this coincided with a rapid corrosion rate of 3.5±0.3 mm/year. Microbial community analysis based on 16S rRNA gene sequencing indicated the absence of sulfide-oxidizing microorganisms in the corrosion layer. The chemical analysis of corrosion products supported the reaction of cement with sulfuric acid formed by the chemical oxidation of H2S. The results suggested that chemically induced oxidation of H2S can lead to the rapid corrosion of new concrete sewers within a few weeks. Generally, chemically induced corrosion plays an important role in the initiation stage of concrete corrosion before the development of sulfide oxidizing microorganisms (SOM). Once SOM is well developed, the microbially induced concrete corrosion (MICC) predominately contributes to the corrosion of concrete sewers. This thesis proposed to incorporate nitrite as an admixture into concrete for MICC control in sewers. Prior to the corrosion resistance test, the feasibility of applying nitrite admixed concrete for sewer structures was investigated. The impacts of calcium nitrite addition and its dosages on the mechanical properties were evaluated through standard tests and the anaerobic sewer biofilm communities developed on concrete surfaces were analyzed by DNA sequencing. The results revealed that setting time and water demand for normal consistency were reduced, but slump, drying shrinkage, and apparent volume of permeable voids increased with the calcium nitrite dosage up to 4% weight of cement. The cumulative leached fraction of nitrite, 28-day compressive 'Distinct microbially induced concrete corrosion at the tidal region of reinforced concrete sewers', Water Research 150, 392-402. (2019). vii

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Enrichment of nitrite-dependent anaerobic methane oxidizing bacteria in a membrane bioreactor

Cited by 78 publications

References 38 publications

Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Interactions between anaerobic ammonium- and methane-oxidizing microorganisms in a laboratory-scale sequencing batch reactor

Development of Active Solids Activator (Pellet) Using Local Culture from Badung River, Bali to Enhance Nitrification Process of Goat Wastewater

Understanding and controlling of concrete corrosion in sewers

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