Enrichment of ovarian cancer stem‐like cells is associated with epithelial to mesenchymal transition through an miRNA‐activated <scp>AKT</scp> pathway

Luo, Xin; Dong, Zhihao; Chen, Yang; Liu, Yang; Lai, Dongmei

doi:10.1111/cpr.12038

Cited by 73 publications

(63 citation statements)

References 42 publications

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“…Here, we reported that overexpression of miR-200c results in low levels of p-PI3K and p-AKT and that this phenomenon could be rescued by Sox2. Luo and colleagues have reported that the miR-20a/miR-200c-PTEN-AKT axis regulates EMT-associated CSC enrichment of epithelial ovarian cancer (EOC) cells (47). In this axis, miR200c expression was significantly higher in EOC spheroid cells, which produce large numbers of CSC cells, and activated the PI3K-AKT pathway.…”

Section: Discussionmentioning

confidence: 99%

Regulation of Colorectal Carcinoma Stemness, Growth, and Metastasis by an miR-200c-Sox2–Negative Feedback Loop Mechanism

Xue

et al. 2014

Clinical Cancer Research

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Purpose: To elucidate a novel mechanism of miR-200c in the regulation of stemness, growth, and metastasis in colorectal carcinoma (CRC).Experimental Design: Quantitative reverse transcription PCR was used to quantify miR-200c expression in CRC cell lines and tissues. A luciferase assay was adopted for the target evaluation. The functional effects of miR-200c in CRC cells were assessed by its forced or inhibited expression using lentiviruses.Results :

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Section: Discussionmentioning

confidence: 99%

Regulation of Colorectal Carcinoma Stemness, Growth, and Metastasis by an miR-200c-Sox2–Negative Feedback Loop Mechanism

Xue

et al. 2014

Clinical Cancer Research

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“…Ovarian CSLCs were enriched as previously described (21). Upon reaching 80% confluence, the SKOV3 cells were dissociated by 0.25% Trypsin-EDTA (Life Technologies, Carlsbad, CA, USA) for 1-2 min at 37˚C.…”

Section: Enrichment Of Cslcsmentioning

confidence: 99%

“…The migratory ability of the cells was determined by Transwell assay as previously described (21). Briefly, the cells were seeded into the top chamber of 8.0 µm Transwell invasion chambers (Corning Inc., Corning, NY, USA) in serum-free medium.…”

Section: Enrichment Of Cslcsmentioning

confidence: 99%

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The marine-derived fungal metabolite, terrein, inhibits cell proliferation and induces cell cycle arrest in human ovarian cancer cells

Chen

Wang

Shen

et al. 2014

International Journal of Molecular Medicine

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Abstract. The difficulties faced in the effective treatment of ovarian cancer are multifactorial, but are mainly associated with relapse and drug resistance. Cancer stem-like cells have been reported to be an important contributor to these hindering factors. In this study, we aimed to investigate the anticancer activities of a bioactive fungal metabolite, namely terrein, against the human epithelial ovarian cancer cell line, SKOV3, primary human ovarian cancer cells and ovarian cancer stem-like cells. Terrein was separated and purified from the fermentation metabolites of the marine sponge-derived fungus, Aspergillus terreus strain PF26. Its anticancer activities against ovarian cancer cells were investigated by cell proliferation assay, cell migration assay, cell apoptosis and cell cycle assays. The ovarian cancer stem-like cells were enriched and cultured in a serum-free in vitro suspension system. Terrein inhibited the proliferation of the ovarian cancer cells by inducing G 2 /M phase cell cycle arrest. The underlying mechanisms involved the suppression of the expression of LIN28, an important marker gene of stemness in ovarian cancer stem cells. Of note, our study also demonstrated the ability of terrein to inhibit the proliferation of ovarian cancer stem-like cells, in which the expression of LIN28 was also downregulated. Our findings reveal that terrein (produced by fermention) may prove to be a promising drug candidate for the treatment of ovarian cancer by inhibiting the proliferation of cancer stem-like cells.

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“…Increasing evidence has suggested that miRNAs play crucial roles in many biological processes, such as development, cell proliferation, invasion, migration and differentiation 20, 21, 22, 23, 24. Mutations of miRNA‐encoding genes or aberrant expression of miRNAs have been well described in various tumours, including gastric, breast and lung cancers, as well as glioblastoma and melanoma 25, 26, 27.…”

Section: Introductionmentioning

confidence: 99%

The role of microRNAs in intrahepatic cholangiocarcinoma

Shen

Chan

et al. 2016

J Cellular Molecular Medi

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Intrahepatic cholangiocarcinoma (ICC) is the second most common primary hepatic malignancy with poor prognosis. Despite improvements in its diagnosis and therapy, the prognosis for ICC patients remains poor. An improved understanding of ICC pathogenesis and consequential identification of novel therapeutic targets would improve the prognosis of ICC patients. MicroRNAs (miRNAs) are a class of highly conserved, endogenous, small non‐coding RNA molecules of 18–23 nucleotides in length, which regulate gene expression through complementary base‐pairing with target messenger RNAs and subsequent gene silencing. Several studies have shown deregulated expression of miRNAs in ICC cell lines and tissues, in which these miRNAs play important roles in ICC apoptosis, cell proliferation, invasion, migration and metastasis. In this review, we illustrate the potential role of miRNA in the pathogenesis of ICC and explore the possibilities of using miRNAs as prognostic and diagnostic markers, as well as therapeutic targets in ICC.

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Enrichment of ovarian cancer stem‐like cells is associated with epithelial to mesenchymal transition through an miRNA‐activated AKT pathway

Abstract: Our findings demonstrate that EMT contributed to enrichment of ovarian CSCs in vitro, making EMT targeting in epithelial ovarian cancer a novel therapeutic option.

Cited by 73 publications

References 42 publications

Regulation of Colorectal Carcinoma Stemness, Growth, and Metastasis by an miR-200c-Sox2–Negative Feedback Loop Mechanism

Regulation of Colorectal Carcinoma Stemness, Growth, and Metastasis by an miR-200c-Sox2–Negative Feedback Loop Mechanism

The marine-derived fungal metabolite, terrein, inhibits cell proliferation and induces cell cycle arrest in human ovarian cancer cells

The role of microRNAs in intrahepatic cholangiocarcinoma

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