Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule

Murray, James; Sim, Jaehwan; Oh, Kyunghoon; Sung, Gihyun; Lee, Ara; Shrinidhi, Annadka; Thirunarayanan, Ayyavu; Shetty, Dinesh; Kim, Kimoon

doi:10.1002/ange.201611894

Cited by 11 publications

(5 citation statements)

References 44 publications

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“…In this work, one segment of the reagent labeled the proteins of interest, while the other side contained a ferrocene moiety for CB7binding. [20] This strategy can be applied to a variety of applications, with a ferrocene tag substituted for the traditional biotin handle.…”

Section: Supramolecular Protein Immobilization and Detectionmentioning

confidence: 99%

Supramolecular strategies for protein immobilization and modification

Finbloom

Francis

2018

Current Opinion in Chemical Biology

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Protein immobilization and modification are widely used techniques in the fields of chemical biology and biomaterials science. While covalent strategies based on small molecules are traditionally used, supramolecular chemistry offers numerous useful opportunities for guiding the modification locations on complex protein landscapes and introducing different degrees of reversibility into the products. In this opinion, we highlight recent advances in using supramolecular interactions, particularly host-guest chemistry, for controlling protein modification and immobilization. We discuss supramolecular strategies for protein-conjugate purification and capture, as well as for protein modification via host-guest interactions and metal coordination. Lastly, we address recent advances in utilizing supramolecular interactions to direct covalent protein modification. These examples of supramolecular chemical biology present opportunities to advance a wide range of applications, including proteomics and drug delivery.

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Section: Supramolecular Protein Immobilization and Detectionmentioning

confidence: 99%

Supramolecular strategies for protein immobilization and modification

Finbloom

Francis

2018

Current Opinion in Chemical Biology

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“…issues, and various cancers [8]. These benefits are often attributed to their capacity to target oxidative stress-related pathways and the intricate regulatory network governing the inflammatory processes [9].…”

Section: Introductionmentioning

confidence: 99%

Advancements in Drug Delivery Systems for Natural Compounds Targeting Metabolic Disorders Associated with Inflammation

Sam,

Neal

2023

Arch Gastroenterol Res

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The global incidence of metabolic disorders is on the rise, posing a significant challenge to public health. With remarkable advancements in diagnostic tools and clinical procedures, our understanding of the etiology and underlying pathophysiology of these disorders has expanded considerably. Furthermore, the utilization of in vitro and in vivo experimental models, preceding clinical investigations, has catalyzed numerous breakthroughs in biomedicine, particularly in the identification and development of potential drug candidates for the management of metabolic disorders. Natural compounds isolated from various sources have garnered extensive attention as prospective drug candidates for the treatment of conditions such as diabetes, obesity, heart-related diseases, and cancer. This interest is partly attributed to their inherent antioxidant and anti-inflammatory properties. Concurrently, intensive research efforts have been directed towards enhancing the bioactivity and bioavailability of these compounds through selected drug delivery strategies. In this article, we provide valuable insights into recent advancements that shed light on the role of inflammatory-mediated responses in the initiation of metabolic disorders, with a specific focus on conditions like diabetes mellitus, obesity, heart-related diseases, cancer, and related drug delivery systems. Additionally, we explore the promising potential of natural products in managing these metabolic disorders. Furthermore, we present lists of potential biological targets suitable for high throughput screening in the drug discovery and development process. Finally, we delve into the findings gleaned from preclinical and clinical studies, setting the stage for the identification of suitable approaches in the realm of phytochemical drug delivery systems that hold promise for the treatment of metabolic disorders.

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“…Many host-guest pairs have been used in 3 guest-conjugated targets in complex biological media. 17,18,19,20,21,22,2324,25,26,27,28,29,30 It is even more challenging to achieve in situ capture in living animals, and there are only very limited successful examples via employing either monovalent 31,32 or multivalent 33,34 systems. In situ capture requires the host-guest pairs to be bioorthogonal, that is, the host-guest pairs are not interfered by biomolecules and salt ions.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Recognition-Based Bioorthogonal Host–Guest Pairs for Cell Targeting and Tissue Imaging in Living Animals

Sun

et al. 2022

CCS Chem

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Bioconjugation methods offer very important tools in studying biological systems. Synthetic host-guest pairs provide an alternative and complementary conjugation method to bioorthogonal reactions and biological association pairs. Nevertheless, the macrocyclic hosts that can be used for in situ capture are very limited and often rely on extremely high binding affinities. Herein, we report an alternative bioorthogonal host-guest pair that relies on highly selective molecular recognition in water. The host, namely amide naphthotube, possesses a biomimetic cavity with inward-directing hydrogen bonding sites and shows selective and strong binding to the guest (2-phenyl pyrimidine) even in biological media. Through anchoring the tetraphenyl ethylenemodified hosts to cell surfaces, the bioorthogonal host-guest pair can be applied in cell surface recognition, cell-cell interactions and tissue imaging in mice. The bioorthogonality is originated from the high binding selectivity of the biomimetic macrocyclic host, which is different from other known host-guest pairs that have

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Enrichment of Specifically Labeled Proteins by an Immobilized Host Molecule

Cited by 11 publications

References 44 publications

Supramolecular strategies for protein immobilization and modification

Supramolecular strategies for protein immobilization and modification

Advancements in Drug Delivery Systems for Natural Compounds Targeting Metabolic Disorders Associated with Inflammation

Biomimetic Recognition-Based Bioorthogonal Host–Guest Pairs for Cell Targeting and Tissue Imaging in Living Animals

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