This method is a variant of non-destructive multiparametric surface analysis and includes the implementation of hyperspectral and RGB image processing approaches from different angles. This work is based on a fundamental hyperspectral survey system for obtaining data on scanned biological objects in many spectral ranges and with several possible variants of assembling a system with different types of surface illumination with point light and diffuse illumination. The implementation of the technology through the use of a diffused light source provides the diffuse illumination of a biological object with pronounced symptoms of rarefaction on the surface of a biological object—in this case, potato tubers, due to the presence of signs of disease on the potato peel, as well as their deformation. Using broadband lamps, a short-pass filter is located between the source and the object (λ ≤ 400 nm, λ may vary depending on the excitation length), and a long-pass filter (λ ≥ 400 nm) between the root or onion and the chamber. The use of a vision system with a created database containing models of real defects in potato tubers showed a high sorting efficiency, providing an accuracy of sorting by size of 95.4%, and an accuracy by the presence of defects of 93.1%.