Enrichment process of 2,4-D degraders in different soils under upland conditions

Miwa, Noriko; Kuwatsuka, Shozo

doi:10.1080/00380768.1990.10414991

Soil Science and Plant Nutrition

1990

DOI: 10.1080/00380768.1990.10414991

|View full text |Cite

Enrichment process of 2,4-D degraders in different soils under upland conditions

Noriko Miwa

Shozo Kuwatsuka

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1991

2008

Publication Types

Select...

Article5

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 5 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of these compounds are good carbon sources for microorganisms capable of degrading them. For example, 2,4-dichlorophenoxyacetic acid (2,4-D) is known to be a growth substrate for a number of different soil microorganisms (4,(15)(16)(17). Since such synthetic compounds are usually not present in nature and their supply is under human control, they make good models to study microbial resource competition in soil.…”

mentioning

confidence: 99%

Analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria

Holben

Tiedje

1994

Appl Environ Microbiol

View full text Add to dashboard Cite

Competition among indigenous and inoculated 2,4-dichlorophenoxyacetic acid (2,4-D)-degrading bacteria was studied in a native Kansas prairie soil following 2,4-D additions. The soil was inoculated with four different 2,4-D-degrading strains at densities of 103 cells per g of soil; the organisms used were Pseudomonas cepacia DBO1(pJP4) and three Michigan soil isolates, strain 745, Sphingomonas paucimobilis 1443, and Pseudomonas pickettii 712. Following 2,4-D additions, total soil DNA was extracted and analyzed on Southern blots by using a #fdA gene probe which detected three of the strains and another probe that detected the fourth strain, S. paucimobilis 1443, which belongs to a different class of 2,4-D degraders. P. cepacia DBO1(pJP4), a constructed strain, outcompeted the other added strains and the indigenous 2,4-D-degrading populations. The S. paucimobilis population was the secondary dominant population, and strain 745 and P. pickettii were not detected. Relative fitness coeflicients determined in axenic broth cultures predicted the outcome of competition in soil for some but not all strains. Lag time was shown to be a principal determinant of competitiveness among the strains, but the lag times were significantly reduced in mixed broth cultures, which changed the competitive outcome. Plasmids containing the genes for the 2,4-D pathway were important determinants of competitiveness since plasmid pKA4 in P. cepacia DBO1 resulted in the slower growth characteristic of its original host, P. pickettii, rather than the rapid growth observed when this strain harbors pJP4.

show abstract

mentioning

confidence: 99%

Analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria

Holben

Tiedje

1994

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

Degradation of 2,4-DB in Argentinean agricultural soils with high humic matter content

Cuadrado

Merini

Flocco

et al. 2008

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

The dissipation of 4-(2,4-dichlorophenoxy) butyric acid (2,4-DB) in high-humic-matter-containing soils from agricultural fields of the Argentinean Humid Pampa region was studied, employing soil microcosms under different experimental conditions. The added herbicide was dissipated almost completely by soils with and without history of herbicide use by day 28. At 500 ppm, both soils showed the same degradation rates; but at 5-ppm concentration, the chronically exposed soil demonstrated a faster degradation of the herbicide. 2,4-DB addition produced increases in herbicide-degrading bacteria of three and 1.5 orders of magnitude in soils with and without history of herbicide use, respectively, in microcosms with 5 ppm. At 500-ppm concentration, the increase in 2,4-DB degraders was five orders of magnitude after 14 days, independent of the history of herbicide use. No differences were observed in either 2,4-DB degradation rates or in degrader bacteria numbers in the presence and absence of alfalfa plants, in spite of some differential characteristics in patterns of 2,4-DB metabolite accumulation. The main factor affecting 2,4-DB degradation rate would be the history of herbicide use, as a consequence of the adaptation of the indigenous microflora to the presence of herbicides in the field.

show abstract

Fungal bioconversion of 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4-dichlorophenol (2,4-DCP)

et al. 2005

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Enrichment process of 2,4-D degraders in different soils under upland conditions

Cited by 5 publications

References 10 publications

Analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria

Analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria

Degradation of 2,4-DB in Argentinean agricultural soils with high humic matter content

Fungal bioconversion of 2,4-dichlorophenoxyacetic acid (2,4-D) and 2,4-dichlorophenol (2,4-DCP)

Contact Info

Product

Resources

About