Ensifer fredii symbiovar vachelliae nodulates endemic Vachellia gummifera in semiarid Moroccan areas

Phylogeny of symbiotic genes reveals symbiovars within legume-nodulating Paraburkholderia species

“…Based on our analysis of NI values (Table 2), we were also able to suggest a 92% threshold for symbiovar delimitation in the analysis of nodA, nodC, nodAC, nifH, nifHDK and fixABC genes of Paraburkholderia. This cut-off value has also been suggested for symbiotic genes of alpha-rhizobia [6,24].…”

Guidelines for the description of rhizobial symbiovars

“…Several different individual genes have been used for this purpose, though the majority of authors have included nodC and we suggest that a phylogeny of this gene should be included in the description of every new symbiovar. In the genus Bradyrhizobium , a limit of 92.5 % identity of nodC gene sequences served to distinguish symbiovars sojae and pachyrhizi [ 34 ], and a 92 % threshold was suggested for symbiovars in the genus Paraburkholderia [ 35 ], similar to the value proposed for symbiovars of Ensifer fredii [ 36 ]. For a number of other symbiovars in the genus Bradyrhizobium , a limit in NifH amino acid identity around 95 % was found to be suitable [ 25 ].…”