Ensilibility and protein degradation characteristics of forage from mountain grasslands containing tanniferous species

Abstract

Ensiling forage from species‐rich mountain grasslands is challenging. Low concentrations of fermentable carbohydrates and the coarse morphological structure of the forage promote the activity of butyric acid forming bacteria. This is associated with the formation of ammonia from protein degradation, resulting in an insufficient pH decline. On the other hand, forage from species‐rich swards may contain tanniferous plant species which contain varying contents of condensed tannins (CT). Therefore, the silage quality of forage prepared from species‐rich mountain grasslands and the role CT may play in silage fermentation was studied. A set‐up of two long‐term mineral fertilization field experiments, located in the Jura mountains and the Alps in Switzerland were used to obtain forage with contrasting species and chemical composition. Collection was done during both the generative and vegetative growth stage from three differently fertilized swards: unfertilised (“0”), fertilized with phosphorus and potassium (“PK”) or fertilized with PK and nitrogen (“NPK”). The forage was wilted to approximately 37% dry matter (DM), chopped to 2 cm lengths and ensiled for at least 65 days as laboratory‐scale silages. The wilted forage was characterized by concentrations of crude protein between 117 and 130 g/kg DM and water‐soluble carbohydrates varying from 84 to 148 g/kg DM. Concentrations of CT ranged from 6 g to 14 g/kg DM and those of soluble CT from 1.8 to 7.6 g/kg DM. All silages contained butyric acid, irrespective of the type of fertilization or harvest stage (range: 1.5 g to 16 g/kg DM). Concentrations of acetic and lactic acid ranged from 2.1 g to 15.0 g/kg DM and from 21.0 to 44.0 g/kg DM, respectively. Concentrations of unfermented sugar remained high and pH levels were above those expected. Formation of non‐protein‐N (NPN) increased in the range of 130 to 264 g/kg N from wilted to ensiled forage. The negative correlations of CT or soluble CT contents with ammonia‐N or NPN in silage were found in both forage from the generative and vegetative harvests indicating a possible relationship with protein degradation during ensiling.

Topics from the 2023 EGF Symposium: The future role of ley‐farming in cropping systems, and other recent grassland events

“…Plant diversity can reduce vulnerability and increase drought resilience of permanent and sown productive grasslands (Lüscher et al, 2022). In recent years, various papers on multispecies swards were published in this journal that illustrate an increasing research interest in plant diversity and forage species other than grasses, both in temperate climate zones (Baker et al, 2023; Golińska et al, 2024; Ineichen et al, 2020, 2023; Komainda et al, 2022; Niderkorn et al, 2019) and dryland pasture systems (Seeno et al, 2023). This follows earlier work published in a special issue on annual and perennial forage legumes in dryland pasture systems (Smith & Elgersma, 2021).…”