Entamoeba histolytica: Ouabain-insensitive Na+-ATPase activity

Souza, A.M. De; Batista, Evander J.O.; Pinheiro, Ana Acácia de Sá; Carvalhaes, Mariana; Lopes, Anibal Gil; Souza, Wanderley de; Caruso-Neves, Celso

doi:10.1016/j.exppara.2007.04.010

Cited by 7 publications

(8 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neither the Na + /H + exchanger inhibitor ethylisopropylamiloride (EIPA; 20 μM) nor the Na + /K + -ATPase inhibitor ouabain (2 mM) had a significant effect on resting [Na + ] i (Figures 1B and 1C). Furosemide (100 μM), an inhibitor of some protozoal Na + -ATPases (De Souza et al., 2007a; Iizumi et al., 2006), caused a small (2.3 ± 0.7 mM) increase in [Na + ] i (n = 4; p = 0.009) (Figure 1D). Sodium orthovanadate (100 μM), a phosphate analog that inhibits P-type ATPases (Cantley et al., 1978a, 1978b), caused [Na + ] i to undergo a prolonged time-dependent increase (Figure 1E).…”

Section: Resultsmentioning

confidence: 99%

“…Some lower eukaryotes, including other protozoa, use an ENA P-type Na + -ATPase to extrude Na + and thereby maintain a low [Na + ] i (De Souza et al., 2007a; Iizumi et al., 2006; Stiles et al., 2003). Amino acid alignments using the sequences of ENA Na + -ATPase family members from Saccharomyces (ScENA1; CAA98867; 29% identity with PfATP4), Leishmania (LdCA1; AAC19126; 29% identity), Trypanosoma (TcENA1; XP_817442.1; 29% identity), and Entamoeba (Enthist1; XM_652464; 39% identity) revealed homology between these proteins and PfATP4.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Na+ Regulation in the Malaria Parasite Plasmodium falciparum Involves the Cation ATPase PfATP4 and Is a Target of the Spiroindolone Antimalarials

Spillman

Allen

McNamara

et al. 2013

Cell Host & Microbe

202

323

View full text Add to dashboard Cite

SummaryThe malaria parasite Plasmodium falciparum establishes in the host erythrocyte plasma membrane new permeability pathways that mediate nutrient uptake into the infected cell. These pathways simultaneously allow Na+ influx, causing [Na+] in the infected erythrocyte cytosol to increase to high levels. The intraerythrocytic parasite itself maintains a low cytosolic [Na+] via unknown mechanisms. Here we present evidence that the intraerythrocytic parasite actively extrudes Na+ against an inward gradient via PfATP4, a parasite plasma membrane protein with sequence similarities to Na+-ATPases of lower eukaryotes. Mutations in PfATP4 confer resistance to a potent class of antimalarials, the spiroindolones. Consistent with this, the spiroindolones cause a profound disruption in parasite Na+ homeostasis, which is attenuated in parasites bearing resistance-conferring mutations in PfATP4. The mutant parasites also show some impairment of Na+ regulation. Taken together, our results are consistent with PfATP4 being a Na+ efflux ATPase and a target of the spiroindolones.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Na+ Regulation in the Malaria Parasite Plasmodium falciparum Involves the Cation ATPase PfATP4 and Is a Target of the Spiroindolone Antimalarials

Spillman

Allen

McNamara

et al. 2013

Cell Host & Microbe

202

323

View full text Add to dashboard Cite

show abstract

“…Initial experiments involving the heterologous expression of PfATP4 in Xenopus laevis oocyte membranes gave results consistent with PfATP4 having a Ca 2+ -dependent ATPase activity ( Krishna et al., 2001 ); however these results were not replicated in a subsequent study ( Rottmann et al., 2010 ) and, in any case, a Ca 2+ transport function has not been demonstrated directly. Ca 2+ -ATPases are closely related to another class of P-type Na + -ATPases, the type IID ENA-ATPases ( e xitus na trus , translating to exit of salt; (reviewed in Rodriguez-Navarro and Benito, 2010 )), which efflux Na + from cells of lower eukaryotes including fungi, bryophytes and several protozoan parasites (including Trypanosoma ( Caruso-Neves et al., 1999; Iizumi et al., 2006 ), Leishmania ( Stiles et al., 2003; de Almeida-Amaral et al., 2008 ) and Entamoeba ( De Souza et al., 2007 )).…”

Section: Na + Regulation In P Falciparumentioning

confidence: 99%

The malaria parasite cation ATPase PfATP4 and its role in the mechanism of action of a new arsenal of antimalarial drugs

Spillman

Kirk

2015

International Journal for Parasitology: Drugs and Drug Resistan

View full text Add to dashboard Cite

The intraerythrocytic malaria parasite, Plasmodium falciparum, maintains a low cytosolic Na+ concentration and the plasma membrane P-type cation translocating ATPase ‘PfATP4’ has been implicated as playing a key role in this process. PfATP4 has been the subject of significant attention in recent years as mutations in this protein confer resistance to a growing number of new antimalarial compounds, including the spiroindolones, the pyrazoles, the dihydroisoquinolones, and a number of the antimalarial agents in the Medicines for Malaria Venture's ‘Malaria Box’. On exposure of parasites to these compounds there is a rapid disruption of cytosolic Na+. Whether, and if so how, such chemically distinct compounds interact with PfATP4, and how such interactions lead to parasite death, is not yet clear. The fact that multiple different chemical classes have converged upon PfATP4 highlights its significance as a potential target for new generation antimalarial agents. A spiroindolone (KAE609, now known as cipargamin) has progressed through Phase I and IIa clinical trials with favourable results. In this review we consider the physiological role of PfATP4, summarise the current repertoire of antimalarial compounds for which PfATP4 is implicated in their mechanism of action, and provide an outlook on translation from target identification in the laboratory to patient treatment in the field.

show abstract

“…Additionally, it has been shown, in inner kidney cortex, that Ang II inhibits the Na + -ATPase activity, mediated by AT2 receptors through a cholera toxin-sensitive PKA pathway [37]. These receptors are differentially distributed throughout the nephron, from outer to inner renal cortex, leading to a preferential binding of Ang II either to AT1 or AT2 receptors, respectively.…”

Section: Modulation Of the Na+-atpase Activitymentioning

confidence: 99%

“…These receptors are differentially distributed throughout the nephron, from outer to inner renal cortex, leading to a preferential binding of Ang II either to AT1 or AT2 receptors, respectively. Therefore, the predominant effect of Ang II on the Na + -ATPase in outer cortex would be stimulatory [40, 131–133], while in the inner cortex this peptide would have an inhibitory effect [37]. …”

Section: Modulation Of the Na+-atpase Activitymentioning

confidence: 99%

The second sodium pump: from the function to the gene

Rocafull

Thomas

Castillo

2012

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

Transepithelial Na+ transport is mediated by passive Na+ entry across the luminal membrane and exit through the basolateral membrane by two active mechanisms: the Na+/K+ pump and the second sodium pump. These processes are associated with the ouabain-sensitive Na+/K+-ATPase and the ouabain-insensitive, furosemide-inhibitable Na+-ATPase, respectively. Over the last 40 years, the second sodium pump has not been successfully associated with any particular membrane protein. Recently, however, purification and cloning of intestinal α-subunit of the Na+-ATPase from guinea pig allowed us to define it as a unique biochemical and molecular entity. The Na+- and Na+/K+-ATPase genes are at the same locus, atp1a1, but have independent promoters and some different exons. Herein, we spotlight the functional characteristics of the second sodium pump, and the associated Na+-ATPase, in the context of its role in transepithelial transport and its response to a variety of physiological and pathophysiological conditions. Identification of the Na+-ATPase gene (atna) allowed us, using a bioinformatics approach, to explore the tertiary structure of the protein in relation to other P-type ATPases and to predict regulatory sites in the promoter region. Potential regulatory sites linked to inflammation and cellular stress were identified in the atna gene. In addition, a human atna ortholog was recognized. Finally, experimental data obtained using spontaneously hypertensive rats suggest that the Na+-ATPase could play a role in the pathogenesis of essential hypertension. Thus, the participation of the second sodium pump in transepithelial Na+ transport and cellular Na+ homeostasis leads us to reconsider its role in health and disease.

show abstract

Entamoeba histolytica: Ouabain-insensitive Na+-ATPase activity

Cited by 7 publications

References 29 publications

Na+ Regulation in the Malaria Parasite Plasmodium falciparum Involves the Cation ATPase PfATP4 and Is a Target of the Spiroindolone Antimalarials

Na+ Regulation in the Malaria Parasite Plasmodium falciparum Involves the Cation ATPase PfATP4 and Is a Target of the Spiroindolone Antimalarials

The malaria parasite cation ATPase PfATP4 and its role in the mechanism of action of a new arsenal of antimalarial drugs

The second sodium pump: from the function to the gene

Contact Info

Product

Resources

About