Entamoeba spp. diagnosis in patients with inflammatory diarrhea by staining, copro-antigen ELISA and multiplex PCR methods

Gharibi, Zahra; Kazemi, Forough; Tavalla, Mehdi

doi:10.12980/apjtd.7.2017d7-137

Cited by 1 publication

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“…There was a 100% correlation between the antigen detection kit and the conventional nested PCR results for E. histolytica diagnosis in suspected patients (22,23,35). Gharibi et al (44), presented that among 200 patients with dysenteric diarrhea, 17, 30, and 23 were positive for E. his-tolytica/E. dispar using microscopic, coproantigen, and PCR techniques, respectively.…”

Section: Discussionmentioning

confidence: 99%

Detection of Entamoeba histolytica Coproantigen Among Children with Dysentery in Ahvaz, Southwest Iran

Shamsizadeh

Nikfar

Rahdar

2020

Zahedan J Res Med Sci

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: Entamoeba histolytica is one of the important parasitic diseases in many parts of the world, especially the tropical and subtropical regions. The parasite is transmitted through contaminated water and vegetables. The exact diagnosis of infection with the parasite is crucial in many medical laboratories since there are many false positive and negative results in their reports. Therefore, the current study aimed at evaluating and comparing microscopic and coproantigen ELISA (the enzyme-linked immunosorbent assay) results to reach an appropriate test for the correct diagnosis of amoebiasis in children. One hundred stool samples were collected from children under 15 years old with dysenteric diarrhea from April to September 2018. Microscopic tests and coproantigen ELISA were performed on all the samples. The results showed that 5% of the samples had E. histolytica/E. dispar cysts. The findings of ELISA to detect coproantigen did not show any specific E. histolytica antigen in the samples. Hence, all the patients received chemotherapy for shigellosis. E. histolytica infection is not the main causative agent for dysenteric diarrhea in children in the studied area, and laboratory experts should be trained to prevent false-positive reports.

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