Enteral and parenteral nutrition distinctively modulate intestinal permeability and T cell function in vitro

Guzy, Claudia; Schirbel, Anja; Paclik, Daniela; Wiedenmann, Bertram; Dignaß, Axel; Sturm, Andreas

doi:10.1007/s00394-008-0754-3

Cited by 34 publications

(18 citation statements)

References 35 publications

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“…One aspect requires comment. The initial concentration of LEs used in this study was referenced to the in vitro study conducted by C. Guzy group [17]. In that study, 5% PN containing approximately 20% of lipid was used, suggesting that the final concentration of LE was approximately 1%.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we used human gut-derived Caco-2 cells as a model, which has been approved suitable for studying intestinal homeostasis and studying the ingredients of parenteral nutrition in vitro [17]. We investigated the expression of CELF1 in the cells exposed to varying LEs, and explored the possible mechanisms linking CELF1 and LE-induced cell death.…”

Section: Introductionmentioning

confidence: 99%

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Olive Oil-Supplemented Lipid Emulsion Induces CELF1 Expression and Promotes Apoptosis in Caco-2 Cells

Yan

Zhu

Gong

et al. 2017

Cell Physiol Biochem

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Background and Aims: Parenterally-administered lipid emulsion (LE) is a key cause of enterocyte apoptosis under total parenteral nutrition, yet the pathogenesis has not been fully understood. CUGBP, Elav-like family member 1 (CELF1) has been recently identified as a crucial modulator of apoptosis, and thus this study sought to investigate its role in the LE-induced apoptosis in vitro. Methods: Caco-2 cells were used as an in vitro model. The cells were treated with varying LEs derived from soybean oil, olive oil or fish oil, and changes in the apoptosis and CELF1 expression were assessed. Rescue study was performed using transient knockdown of CELF1 with specific siRNA prior to LE treatment. Regulation of CELF1 by LE treatment was studied using quantitative real-time PCR and Western blotting. Results: All the LEs up-regulated CELF1expression and induced apoptosis, but only olive oil-supplemented lipid emulsion (OOLE)-induced apoptosis was attenuated by depletion of CELF1. Up-regulation of apoptosis-inducing factor (AIF) was involved in OOLE-induced CELF1 dependent apoptosis. The protein expression of CELF1 was up-regulated by OOLE in a dose- and time-dependent manner, but the mRNA expression of CELF1 was unchanged. Analysis by polysomal profiling and nascent protein synthesis revealed that the regulation of CELF1 by OOLE treatment was mediated by directly accelerating its protein translation. Conclusion: OOLE-induces apoptosis in Caco-2 cells partially through up-regulation of CELF1.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Olive Oil-Supplemented Lipid Emulsion Induces CELF1 Expression and Promotes Apoptosis in Caco-2 Cells

Yan

Zhu

Gong

et al. 2017

Cell Physiol Biochem

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“…There is evidence that EN and PN, in diseases accompanied by mucosal barrier dysfunction, interact with the intestinal barrier and the immune system, by promoting wound healing and regulating T-cell function, enhancing the mucosal defense against BT and systemic inflammation [69]. There seems to be a diversification in the function of mucosal host defenses between EN and PN [70].…”

Section: Composition Of Enteral and Parenteral Nutrition Solutionsmentioning

confidence: 99%

Artificial Nutrition and Intestinal Mucosal Barrier Functionality

Anastasilakis¹,

Ioannidis

Gkiomisi

et al. 2013

Digestion

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The gastrointestinal tract has a major role in digestion and absorption of nutrients while playing a leading role in defense of the body. It forms a shield against the invasion of various microorganisms or their products (e.g. antigens, toxins) and therefore it is important to establish its integrity and functionality. That depends on the route of administration and the composition of the artificial nutrition. This study concentrates on the influences of different kinds of artificial nutrition in the functionality of the intestinal mucosal barriers. It seems that full macromolecular solutions of enteral nutrition ensure an adequate mucous immune response, while a lack of nutritional stimulus in the lumen leads rapidly to a dysfunction of gastric-associated lymphatic tissue and mucosal immune system. This dysfunction renders the patients susceptible to infections in distant organs, hospital pneumonia, and multiorgan failure of non-infectious etiology. In patients with indication of total parenteral nutrition administration, addition of bombesin or glutamine preserves mucosal immune response and may limit the adverse effects.

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“…Nevertheless, mechanisms underlying the action of enteral nutrition are yet to be fully understood, although several mechanisms have been proposed by many researchers. These mechanisms include improvement of nutritional status (Beatti et al, 1994), down regulation of pro-inflammatory cytokines (de Jong et al, 2007), modification of gut flora (Leach, 2008), anti-inflammatory effects (Fell, 2005), promoting epithelial healing (Fell et al, 2000), decreased gut permeability (Guzy et al, 2009) and decreased antigenic load to the gut (Beatti et al, 1994). The association between diet and IBD is likely to be determined by major components found in the foods.…”

Section: Mechanisms Underlying the Association Of Diet With Ibdmentioning

confidence: 99%

The Role of Diet, Prebiotic and Probiotic in the Development and Management of Inflammatory Bowel Diseases (IBD)

Abdulamir¹,

Zaman²,

Hafidh³

et al. 2012

Inflammatory Bowel Disease - Advances in Pathogenesis and Management

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Enteral and parenteral nutrition distinctively modulate intestinal permeability and T cell function in vitro

Abstract: Our study provides evidence that by promoting wound healing and regulating T cell function, EN, AM, and PN potently interact with the intestinal barrier and immune system, thus justifying its use in diseases accompanied by impaired mucosal barrier function.

Cited by 34 publications

References 35 publications

Olive Oil-Supplemented Lipid Emulsion Induces CELF1 Expression and Promotes Apoptosis in Caco-2 Cells

Olive Oil-Supplemented Lipid Emulsion Induces CELF1 Expression and Promotes Apoptosis in Caco-2 Cells

Artificial Nutrition and Intestinal Mucosal Barrier Functionality

The Role of Diet, Prebiotic and Probiotic in the Development and Management of Inflammatory Bowel Diseases (IBD)

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