Enteric-coated pellets of F4 fimbriae for oral vaccination of suckling piglets against enterotoxigenic Escherichia coli infections

Snoeck, Veerle; Huyghebaert, Nathalie; Cox, Eric; Vermeire, An; Vancaeneghem, Sabine; Remon, Jean Paul; Goddeeris, Bruno

doi:10.1016/j.vetimm.2003.08.003

Cited by 42 publications

(28 citation statements)

References 14 publications

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“…However, incomplete anti-colonization immunity was induced against a challenge strain of ETEC with F18ab fimbriae when the vaccine contained F18ac fimbriae. These results are in contrast to those obtained by Snoeck et al (2003) in which oral vaccination of pigs at 7 and 21 d of age failed to protect against colonization by F4 ETEC. They also demonstrated that milk inhibits the adhesion of F4 fimbriae to the villous brush border, thereby interfering with the induction of local and systemic immunity.…”

Section: Vaccinationcontrasting

confidence: 99%

Subtherapeutic use of antibiotics in pork production: Risks and alternatives

Adjiri-Awere¹,

Lunen²

2005

Can. J. Anim. Sci.

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The purpose of this review is to discuss the issue of subtherapeutic use of antibiotics in pork production and possible alternatives. Specifically, the review describes the potential problem of antibiotic resistance and reviews disease prevention and immune potentiation strategies as alternatives to subtherapeutic antibiotics. In recent years, the use of in-feed subtherapeutic antibiotics by the pork industry for growth promotion and disease prophylaxis has come under increased scrutiny. The potential risk of bacteria acquiring resistance to specific antibiotics and the detrimental effects that this may have on human health has resulted in the banning of certain antibiotics in some European countries. Despite the current interest in the reduction or elimination of subtherapeutic antibiotic use in livestock production, there may be a risk that such a reduction or elimination would have negative effects on animal welfare, nutrient utilization, manure production and economic sustainability. A number of alternatives to subtherapeutic antibiotics appear promising; however, more research is required before they can be considered viable commercial products. Consequently, a systems approach involving nutrition, bacteriology, immunology, pathology and herd management is needed to find a cost-effective and practical way to maximize the efficient production of high-quality pork without the use of subtherapeutic antibiotics. Key words: Pig, pork production, subtherapeutic antibiotics, alternatives

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Section: Vaccinationcontrasting

confidence: 99%

Subtherapeutic use of antibiotics in pork production: Risks and alternatives

Adjiri-Awere¹,

Lunen²

2005

Can. J. Anim. Sci.

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“…FaeG is the major structural subunit for K88 fimbriae (3), and FaeG antigens elicited antibodies blocking K88 fimbrial adherence (26). Experimental vaccines carrying K88 antigens showed some protection against ETEC strains expressing the same fimbriae (25,34,36). FedF is a minor subunit of F18 fimbriae, and it plays a critical role in F18 fimbrial adherence (23).…”

mentioning

confidence: 99%

A Tripartite Fusion, FaeG-FedF-LT₁₉₂A2:B, of Enterotoxigenic Escherichia coli (ETEC) Elicits Antibodies That Neutralize Cholera Toxin, Inhibit Adherence of K88 (F4) and F18 Fimbriae, and Protect Pigs against K88ac/Heat-Labile Toxin Infection

Ruan¹,

Liu²,

Casey³

et al. 2011

Clin. Vaccine Immunol.

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Enterotoxigenic Escherichia coli (ETEC) strains expressing K88 (F4) or F18 fimbriae and heat-labile (LT)and/or heat-stable (ST) toxins are the major cause of diarrhea in young pigs. Effective vaccines inducing antiadhesin (anti-K88 and anti-F18) and antitoxin (anti-LT and anti-ST) immunity would provide broad protection to young pigs against ETEC. In this study, we genetically fused nucleotides coding for peptides from K88ac major subunit FaeG, F18 minor subunit FedF, and LT toxoid (LT 192 ) A2 and B subunits for a tripartite adhesin-adhesin-toxoid fusion (FaeG-FedF-LT 192 A2:B). This fusion was used for immunizations in mice and pigs to assess the induction of antiadhesin and antitoxin antibodies. In addition, protection by the elicited antiadhesin and antitoxin antibodies against a porcine ETEC strain was evaluated in a gnotobiotic piglet challenge model. The data showed that this FaeG-FedF-LT 192 A2:B fusion elicited anti-K88, anti-F18, and anti-LT antibodies in immunized mice and pigs. In addition, the anti-porcine antibodies elicited neutralized cholera toxin and inhibited adherence against both K88 and F18 fimbriae. Moreover, immunized piglets were protected when challenged with ETEC strain 30302 (K88ac/LT/STb) and did not develop clinical disease. In contrast, all control nonvaccinated piglets developed severe diarrhea and dehydration after being challenged with the same ETEC strain. This study clearly demonstrated that this FaeG-FedF-LT 192 A2:B fusion antigen elicited antibodies that neutralized LT toxin and inhibited the adherence of K88 and F18 fimbrial E. coli strains and that this fusion could serve as an antigen for vaccines against porcine ETEC diarrhea. In addition, the adhesin-toxoid fusion approach used in this study may provide important information for developing effective vaccines against human ETEC diarrhea.

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“…Fimbriae of ETEC in a cellulose-based delivery system reduced disease in experimentally infected pigs [150].…”

Section: Other Biodegradable Polymersmentioning

confidence: 99%

Mucosal delivery of vaccines in domestic animals

et al. 2006

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-Mucosal vaccination is proving to be one of the greatest challenges in modern vaccine development. Although highly beneficial for achieving protective immunity, the induction of mucosal immunity, especially in the gastro-intestinal tract, still remains a difficult task. As a result, only very few mucosal vaccines are commercially available for domestic animals. Here, we critically review various strategies for mucosal delivery of vaccines in domestic animals. This includes live bacterial and viral vectors, particulate delivery-systems such as polymers, alginate, polyphosphazenes, immune stimulating complex and liposomes, and receptor mediated-targeting strategies to the mucosal tissues. The most commonly used routes of immunization, strategies for delivering the antigen to the mucosal surfaces, and future prospects in the development of mucosal vaccines are discussed. mucosal / vaccine / livestock / animals / review

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Enteric-coated pellets of F4 fimbriae for oral vaccination of suckling piglets against enterotoxigenic Escherichia coli infections

Cited by 42 publications

References 14 publications

Subtherapeutic use of antibiotics in pork production: Risks and alternatives

Subtherapeutic use of antibiotics in pork production: Risks and alternatives

A Tripartite Fusion, FaeG-FedF-LT₁₉₂A2:B, of Enterotoxigenic Escherichia coli (ETEC) Elicits Antibodies That Neutralize Cholera Toxin, Inhibit Adherence of K88 (F4) and F18 Fimbriae, and Protect Pigs against K88ac/Heat-Labile Toxin Infection

Mucosal delivery of vaccines in domestic animals

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Enteric-coated pellets of F4 fimbriae for oral vaccination of suckling piglets against enterotoxigenic Escherichia coli infections

Cited by 42 publications

References 14 publications

Subtherapeutic use of antibiotics in pork production: Risks and alternatives

Subtherapeutic use of antibiotics in pork production: Risks and alternatives

A Tripartite Fusion, FaeG-FedF-LT192A2:B, of Enterotoxigenic Escherichia coli (ETEC) Elicits Antibodies That Neutralize Cholera Toxin, Inhibit Adherence of K88 (F4) and F18 Fimbriae, and Protect Pigs against K88ac/Heat-Labile Toxin Infection

Mucosal delivery of vaccines in domestic animals

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Product

Resources

About

A Tripartite Fusion, FaeG-FedF-LT₁₉₂A2:B, of Enterotoxigenic Escherichia coli (ETEC) Elicits Antibodies That Neutralize Cholera Toxin, Inhibit Adherence of K88 (F4) and F18 Fimbriae, and Protect Pigs against K88ac/Heat-Labile Toxin Infection