Enteric Nervous System Stem Cells Derived From Human Gut Mucosa for the Treatment of Aganglionic Gut Disorders

Metzger, Marco; Caldwell, C; Barlow, Amanda; Burns, Alan J.; Thapar, Nikhil

doi:10.1053/j.gastro.2009.02.048

Cited by 190 publications

(235 citation statements)

References 31 publications

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“…Autologous ENS stem cells show promise for clinical therapeutic use (El-Nachef and Grikscheit 2014). Multiple reports show successful transplantation of ENS cells into aganglionic colon; however, incorporation into aganglionic hTEC in vivo has yet to be achieved (Metzger et al 2009;Bitar and Raghavan 2014).…”

Section: Tissue Engineering Functional Gastrointestinal Regionsmentioning

confidence: 99%

Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells

Trecartin

Grikscheit

2017

Cold Spring Harb Perspect Med

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Section: Tissue Engineering Functional Gastrointestinal Regionsmentioning

confidence: 99%

Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells

Trecartin

Grikscheit

2017

Cold Spring Harb Perspect Med

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“…Neural crest stem cells can be isolated from gut of animals (Natarajan et al 1999;Bondurand et al 2003;Kruger et al 2002;Almond et al 2007;Metzger et al 2009). NCSCs are a multipotent, migratory cell population unique to vertebrates that gives rise to many cell lineages including melanocytes, craniofacial cartilage and bone, smooth muscle, peripheral and enteric neurons and glia (Farlie et al 2004;Heanue and Pachnis 2007;Nagoshi et al 2008).…”

Section: Neural Crest Stem Cells (Ncscs)mentioning

confidence: 99%

Intestinal stem cells and stem cell-based therapy for intestinal diseases

2014

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Currently, many gastrointestinal diseases are a major reason for the increased mortality rate of children and adults every year. Additionally, these patients may cope with the high cost of the parenteral nutrition (PN), which aids in the long-term survival of the patients. Other treatment options include surgical lengthening, which is not sufficient in many cases, and intestinal transplantation. However, intestinal transplantation is still accompanied by many challenges, including immune rejection and donor availability, which may limit the transplant's success. The development of more safe and promising alternative treatments for intestinal diseases is still ongoing. Stem cell-based therapy (SCT) and tissue engineering (TE) appear to be the next promising choices for the regeneration of the damaged intestine. However, suitable stem cell source is required for the SCT and TE process. Thus, in this review we discuss how intestinal stem cells (ISCs) are a promising cell source for small intestine diseases. We will also discuss the different markers were used to identify ISCs. Moreover, we discuss the dominant Wnt signaling pathway in the ISC niche and its involvement in some intestinal diseases. Additionally, we discuss ISC culture and expansion, which are critical to providing enough cells for SCT and TE. Finally, we conclude and recommend that ISC isolation, culture and expansion should be considered when SCT is a treatment option for intestinal disorders. Therefore, we believe that ISCs should be considered a cell source for SCT for many gastrointestinal diseases and should be highlighted in future clinical applications.

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“…Metzger and cols., isolated by endoscopy, intestinal epithelial cells from 9 months to 17 years old children. They culture these cells to give rise to neurospheres and then transplanted them into the aganglionic chicken intestine and human hindgut generating ganglionic-type structures, neurons and glia [21].…”

Section: Introductionmentioning

confidence: 99%

ET3 Promotes the Glial Phenotype in Enteric Neural Progenitors When Used in Parallel or after GDNF Stimulus

Carreón-Rodríguez

Martínez-Martínez

Rodríguez-Valentín

2017

JSRT

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GDNF and ET3 show a complex interaction through the enteric nervous system development. GDNF stimulates the proliferation and differentiation of neural precursors, whereas ET3 blocks differentiation induced by GDNF thereby maintaining a constant pool of precursors. However, ET3 and its receptor play a critical role in the formation of enteric progenitor cells. The effects of these factors seem to compete at times and be complementary in others. Therefore, the aim of this study was to identify the synchronization between these factors to determine the effect on the differentiation and proliferation of neuronal and glial phenotypes in vitro, to generate populations with cell density and state of development suitable for colonization, adaptation and proper growth in transplantation. This work provides evidence regarding the different effects which ET3 on proliferation and neuronal and glial differentiation, depending on at which interact with the action of GDNF.

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Enteric Nervous System Stem Cells Derived From Human Gut Mucosa for the Treatment of Aganglionic Gut Disorders

Cited by 190 publications

References 31 publications

Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells

Tissue Engineering Functional Gastrointestinal Regions: The Importance of Stem and Progenitor Cells

Intestinal stem cells and stem cell-based therapy for intestinal diseases

ET3 Promotes the Glial Phenotype in Enteric Neural Progenitors When Used in Parallel or after GDNF Stimulus

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