Enterobacteriaceae in soils and atmospheric dust aerosol accumulations of Moscow city

Глушакова, А. М.; Качалкин, А. В.; Прокофьева, Т.В.; Лысак, Л. В.

doi:10.1016/j.crmicr.2022.100124

Cited by 10 publications

(1 citation statement)

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“…The corresponding bla IMP genes are usually encoded on class 1 integrons and have spread to several clinically relevant bacteria, such as Enterobacteriaceae and Acinetobacter species [ 22 , 23 , 25 , 26 , 27 ]. Enterobacteriaceae , including carbapenemase-producing ones, have been identified in many environments, including rivers, estuaries and coastal waters [ 28 , 29 ], marine salterns [ 30 ], urban topsoils and dust aerosols [ 31 ], or edible vegetables [ 32 , 33 ]. Acinetobacter species are likewise ubiquitous in the environment, such as aqueous and soil habitats, food and animals [ 34 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Fate of Horizontal-Gene-Transfer Markers and Beta-Lactamase Genes during Thermophilic Composting of Human Excreta

et al. 2023

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Thermophilic composting is a suitable treatment for the recycling of organic wastes for agriculture. However, using human excreta as feedstock for composting raises concerns about antibiotic resistances. We analyzed samples from the start and end of a thermophilic composting trial of human excreta, together with green cuttings and straw, with and without biochar. Beta-lactamase genes blaCTX-M, blaIMP, and blaTEM conferring resistance to broad-spectrum beta-lactam antibiotics, as well as horizontal gene transfer marker genes, intI1 and korB, were quantified using qPCR. We found low concentrations of the beta-lactamase genes in all samples, with non-significant mean decreases in blaCTX-M and blaTEM copy numbers and a mean increase in blaIMP copy numbers. The decrease in both intI1 and korB genes from start to end of composting indicated that thermophilic composting can decrease the horizontal spread of resistance genes. Thus, thermophilic composting can be a suitable treatment for the recycling of human excreta.

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