Enterocin CRL35 InhibitsListeria monocytogenesin a Murine Model

Abstract

Listeria monocytogenes is a foodborne pathogen causative of opportunistic infections. Listeriosis is associated with severe infections in pregnant women causing abortion or neonatal listeriosis. An alternative to antibiotics are safe novel bacteriocins peptides such as enterocin CRL35 with strong antilisterial activity produced by Enterococcus mundtii CRL35. In the present paper, our goal is to study the effectiveness of this peptide and the producer strain in a murine model of pregnancy-associated listeriosis. A single dose of 5×10(9) colony-forming unit of L. monocytogenes FBUNT (Faculty of Biochemistry-University of Tucumán) resulted in translocation of pathogen to liver and spleen of BALB/c pregnant mice. The maximum level of Listeria was observed on day 3 postinfection. Interestingly, the intragastric administration of enterocin CRL35 significantly reduced the translocation of the pathogen to vital organs. On the other hand, the preadministration of E. mundtii CRL35 slightly inhibited this translocation. Listeria infection caused a significant increase in polymorphonuclear leukocytes at day 3 postinfection compared to the noninfected group. This value was reduced after the administration of enterocin CRL35. No significant changes were observed in either white blood cells or lymphocytes counts. Based on the data presented in the present work enterocin CRL35 would be a promising alternative for the prevention of Listeria infections.

Bacteriocin-Producing Enterococcus faecium LCW 44: A High Potential Probiotic Candidate from Raw Camel Milk

“…This intestinal segment is also the entry portal of several pathogenic bacteria such as Salmonella and Listeria (Siebers and Finlay, 1996; Vázquez-Boland et al, 2001). Several groups have examined the possibility of reducing the incidence of these infections using bacteriocin producers such as Enterococcus mundtii CRL 35 (Salvucci et al, 2011), Lactococcus lactis DPC 6520 (Dobson et al, 2011) or Pediococcus acidilactici UL 5 (Dabour et al, 2009). In situ production of bacteriocin does indeed appear to provide protection against pathogenic microorganisms (Corr et al, 2007) and to enhance niche competition in the mammalian gastrointestinal tract (Kommineni et al, 2015).…”

Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review

“…Avian egg yolk antibodies against Listeria have shown promising results by inhibiting Listeria in various samples tested (Sui et al 2011) (Figure 3). Enterocin CRL35, a peptide possessing bacteriocin property, when tested in a murine model was found to have anti-listerial activity (Salvucci et al 2012). Bacteriocin activity has been attributed to a 28-mer fragment of this enterocin, demanding further insights into the peptide for finding a novel area for preventing the food-borne listerial infections (Masias et al 2015).…”

Survival and Metabolic Activity of Pediocin Producer Pediococcus acidilactici UL5: Its Impact on Intestinal Microbiota and Listeria monocytogenes in a Model of the Human Terminal Ileum

“…Other bacteriocin-producing strains failed to prevent L. monocytogenes infection. Enterococcus mundtii CRL35 (a producer of enterocin CRL35) fed to mice 5 hours before challenge only delayed translocation of the pathogens to the liver and spleen [60]. No improvement was obtained by feeding during the 3 days prior to challenge.…”