Enterococcus durans EP1 a Promising Anti-inflammatory Probiotic Able to Stimulate sIgA and to Increase Faecalibacterium prausnitzii Abundance

Abstract

Enterococcus species, principally Enterococcus faecium are used as probiotics since a long time with preference in animal applications but safety considerations were updated and also new uses as probiotics can be envisaged. Fifteen Enterococcus strains isolated from different foods were identified and analyzed for virulence factors and antibiotic resistance. Three Enterococcus durans strains were selected to study their immunomodulatory properties on PBMC and Caco2 cells. Two strains presented a profile toward a mild inflammatory Th1 response considering TNF-α/IL-10 and IL-1β/IL-10 cytokines ratios. The third strain EP1, presented an anti-inflammatory potential and was selected for in vivo studies. In mice, the strain was well tolerated and did not cause any adverse effects. EP1 administration increased the amount of IgA+ cells in mesenteric lymph node (MLN) after 7 days of administration. In fecal samples, the IgA content increased gradually and significantly from day 7 to day 21 in treated group. Additionally, IL-17, IL-6, IL-1β, IFN-γ, and CXCL1 gene expression significantly decreased on day 21 in Peyer’s patches and IL-17 decreased in MLN. Mice treated with the probiotic showed significant lower mRNA levels of pro-inflammatory cytokines and mucins in the ileum at day 7 while their expression was normalized at day 21. Colonic expression of il-1β, il6, and mucins remain diminished at day 21. Ileum and colon explants from treated mice stimulated in vitro with LPS showed a significant reduction in IL-6 and an increase in IL-10 secretion suggesting an in vivo protective effect of the probiotic treatment against a proinflammatory stimulus. Interestingly, analysis of feces microbiota demonstrated that EP1 administration increase the amount of Faecalibacterium prausnitzii, a butyrate-producing bacteria, which is known for its anti-inflammatory effects. In conclusion, we demonstrated that EP1 strain is a strong sIgA inducer and possess mucosal anti-inflammatory properties. This strain also modulates gut microbiota increasing Faecalibacterium prausnitzii, a functionally important bacterium. Thus, E. durans EP1 is not only a good candidate to increases F. prausnitzii in some cases of dysbiosis but can also be interesting in gut inflammatory disorders therapy.

Sortase-Dependent Proteins Promote Gastrointestinal Colonization by Enterococci

“…As with other studies (36,38), our adhesion assays were performed using porcine mucin. Even though galactose, N-acetylglucosamine, fucose, and sialic acid are the most common carbohydrates in both porcine and mouse mucin (39,40), the structures of the mucin glycans differ between mouse mucin and porcine mucin.…”

Influence of Intestinal Indigenous Microbiota on Intrafamilial Infection by Helicobacter pylori in Japan

“…A higher abundance of F. prausnitzii was detected in the microbiota of H. pylori -negative parents. F. prausnitzii has been reported to be one of the bacterial species possessing anti-inflammatory properties ( 25 , 26 ), and higher abundances of this species were detected preferentially in Japanese subjects with lean body mass ( 27 ).…”

The Genus Enterococcus: Between Probiotic Potential and Safety Concerns—An Update

“…durans TN-3 suppressed the development of dextran sodium sulfate DSS colitis via the induction of IL-10 producing Treg cells (Kanda et al, 2016 ). Another recent study have reported that E. durans EP1 modulates gut microbiota by increasing Faecalibacterium prausnitzii , a functionally important bacterium in some cases of dysbiosis (Carasi et al, 2017 ).…”

“…This novel anti-inflammatory bacterium may be preferentially useful as a prophylactic treatment to avoid inflammatory bowel disease (Avram-Hananel et al, 2010 ). More recently, administration of E. durans EP1 was found to increase the amount of Faecalibacterium prausnitzii , a butyrate-producing bacteria, which is known for its anti-inflammatory effects (Carasi et al, 2017 ).…”