Enterococcus faecium Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and Utilization

Wang, Weiwei; Cai, Huiyi; Zhang, Anrong; Chen, Zhimin; Chang, Wenhuan; Liu, Guohua; Deng, Xuejuan; Bryden, W. L.; Zheng, Aijuan

doi:10.3390/ani10071232

Cited by 24 publications

(29 citation statements)

References 51 publications

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“…In addition, the salmonella-associated alteration of the gut microbiota could be a result of either pathogen-commensal microbiota interaction or host intestinal mucosal immune responses to the pathogen or even a combination of both ( 75 , 77 ). As previously reported, the supplementation of E. faecium significantly altered the gut microbiota composition of broilers and enriched the relative abundance of short-chain fatty acid-producing microbes ( 78 ). Also, E. faecium is a natural commensal bacteria of the poultry gastrointestinal tract and is commercially used as a probiotic in poultry diets ( 47 ), previous studies reported that numerous types of probiotic (e.g., Bacillus subtilis , Lactobacillus , and E. faecium ) supplementation can alter the community and structure of the gut microbiota and improve its diversity ( 11 , 79 – 81 ).…”

Section: Discussionsupporting

confidence: 64%

Intestinal Mucosal Immunity-Mediated Modulation of the Gut Microbiome by Oral Delivery of Enterococcus faecium Against Salmonella Enteritidis Pathogenesis in a Laying Hen Model

et al. 2022

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Enterococcus faecium (E. faecium) is a protective role that has crucial beneficial functions on intestinal homeostasis. This study aimed to investigate the effects of E. faecium on the laying performance, egg quality, host metabolism, intestinal mucosal immunity, and gut microbiota of laying hens under the Salmonella Enteritidis (S. Enteritidis) challenge. A total of 400 45-week-old laying hens were randomly divided into four treatments (CON, EF, SCON, and SEF groups) with five replicates for each group and 20 hens per replicate and fed with a basal diet or a basal diet supplemented with E. faecium (2.5 × 108 cfu/g feed). The experiment comprised two phases, consisting of the pre-salmonella challenged phase (from day 14 to day 21) and the post-salmonella challenged phase (from day 21 to day 42). At day 21 and day 22, the hens in SCON and SEF groups were orally challenged with 1.0 ml suspension of 109 cfu/ml S. Enteritidis (CVCC3377) daily, whereas the hens in CON and EF groups received the same volume of sterile PBS. Herein, our results showed that E. faecium administration significantly improved egg production and shell thickness during salmonella infection. Also, E. faecium affected host lipid metabolism parameters via downregulating the concentration of serum triglycerides, inhibited oxidative stress, and enhanced immune functions by downregulating the level of serum malondialdehyde and upregulating the level of serum immunoglobulin G. Of note, E. faecium supplementation dramatically alleviated intestinal villi structure injury and crypt atrophy, and improved intestinal mucosal barrier injuries caused by S. Enteritidis challenge. Moreover, our data revealed that E. faecium supplementation ameliorated S. Enteritidis infection-induced gut microbial dysbiosis by altering the gut microbial composition (reducing Bacteroides, Desulfovibrio, Synergistes, and Sutterella, and increasing Barnesiella, Butyricimonas, Bilophila, and Candidatus_Soleaferrea), and modulating the gut microbial function, such as cysteine and methionine metabolism, pyruvate metabolism, fatty acid metabolism, tryptophan metabolism, salmonella infection, and the PI3K-Akt signaling pathway. Taken together, E. faecium has a strong capacity to inhibit the S. Enteritidis colonization of hens. The results highlight the potential of E. faecium supplementation as a dietary supplement to combat S. Enteritidis infection in animal production and to promote food safety.

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Section: Discussionsupporting

confidence: 64%

Intestinal Mucosal Immunity-Mediated Modulation of the Gut Microbiome by Oral Delivery of Enterococcus faecium Against Salmonella Enteritidis Pathogenesis in a Laying Hen Model

et al. 2022

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“…Recently, it has been demonstrated that the interactions between gut microbiota and phosphorus/vitamin D were crucial in bone development and mineralization. 36 , 37 The positive effects of Escherichia Faecium on phosphorus metabolism were associated with changes in Escherichia Shigella in broilers, which improved gut phosphorus absorption and bone-forming metabolic activities, and decreased phosphorus excretion. 36 However, bone minerals and apatite also serve as a dumping ground for trace elements and drugs, which seriously affects the bone health.…”

Section: Discussionmentioning

confidence: 99%

“… 36 , 37 The positive effects of Escherichia Faecium on phosphorus metabolism were associated with changes in Escherichia Shigella in broilers, which improved gut phosphorus absorption and bone-forming metabolic activities, and decreased phosphorus excretion. 36 However, bone minerals and apatite also serve as a dumping ground for trace elements and drugs, which seriously affects the bone health. 38 Vitamin D3 supplementation decreased the relative abundance of Escherichia Shigella in the upper gastrointestinal tract.…”

Section: Discussionmentioning

confidence: 99%

Gut microbiota is associated with bone mineral density

Cheng

Wen

Yang

et al. 2021

Bone & Joint Research

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Aims Despite the interest in the association of gut microbiota with bone health, limited population-based studies of gut microbiota and bone mineral density (BMD) have been made. Our aim is to explore the possible association between gut microbiota and BMD. Methods A total of 3,321 independent loci of gut microbiota were used to calculate the individual polygenic risk score (PRS) for 114 gut microbiota-related traits. The individual genotype data were obtained from UK Biobank cohort. Linear regressions were then conducted to evaluate the possible association of gut microbiota with L1-L4 BMD (n = 4,070), total BMD (n = 4,056), and femur total BMD (n = 4,054), respectively. PLINK 2.0 was used to detect the single-nucleotide polymorphism (SNP) × gut microbiota interaction effect on the risks of L1-L4 BMD, total BMD, and femur total BMD, respectively. Results We detected five, three, and seven candidate gut microbiota-related traits for L1-L4 BMD, total BMD, and femur BMD, respectively, such as genus Dialister (p = 0.004) for L1-L4 BMD, and genus Eisenbergiella (p = 0.046) for total BMD. We also detected two common gut microbiota-related traits shared by L1-L4 BMD, total BMD, and femur total BMD, including genus Escherichia Shigella and genus Lactococcus. Interaction analysis of BMD detected several genes that interacted with gut microbiota, such as phospholipase D1 ( PLD1) and endomucin ( EMCN) interacting with genus Dialister in total BMD, and COL12A1 and Discs Large MAGUK Scaffold Protein 2 ( DLG2) interacting with genus Lactococcus in femur BMD. Conclusion Our results suggest associations between gut microbiota and BMD, which will be helpful to further explore the regulation mechanism and intervention gut microbiota of BMD. Cite this article: Bone Joint Res 2021;10(11):734–741.

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“…The expression levels of IL-2 and INF-γ are downregulated ( 75 ). E. faecium supplementation upregulates the expression of intestinal-type IIb sodium-dependent phosphate cotransporter mRNA, increases the concentration of serum alkaline phosphatase, changes the gut microbiota populations, and increases the utilization of phosphorus ( 76 ). Bacillus subtilis BYS2 supplementation improves the production performance, immunity, and disease resistance; promotes innate immune response, increases the expression levels of interferon (IFN)-stimulated genes and β-defensins, and upregulates inflammatory cytokines ( 77 ).…”

Section: Antibiotic-free Management Strategies In Broilersmentioning

confidence: 99%

Progress on Gut Health Maintenance and Antibiotic Alternatives in Broiler Chicken Production

Zhu

Sun²,

Zhang

et al. 2021

Front. Nutr.

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The perturbation of gut health is a common yet unresolved problem in broiler chicken production. Antibiotics used as growth promoters have remarkably improved the broiler production industry with high feed conversion efficiency and reduced intestinal problems. However, the misuse of antibiotics has also led to the increase in the development of antibiotic resistance and antibiotic residues in the meat. Many countries have enacted laws prohibiting the use of antibiotics in livestock production because of the increasing concerns from the consumers and the public. Consequently, one of the most significant discussions in the poultry industry is currently antibiotic-free livestock production. However, the biggest challenge in animal husbandry globally is the complete removal of antibiotics. The necessity to venture into antibiotic-free production has led researchers to look for alternatives to antibiotics in broiler chicken production. Many strategies can be used to replace the use of antibiotics in broiler farming. In recent years, many studies have been conducted to identify functional feed additives with similar beneficial effects as antibiotic growth promoters. Attention has been focused on prebiotics, probiotics, organic acids, emulsifiers, enzymes, essential oils, tributyrin, and medium-chain fatty acids. In this review, we focused on recent discoveries on gut health maintenance through the use of these functional feed additives as alternatives to antibiotics in the past 10 years to provide novel insights into the design of antibiotic-free feeds.

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Enterococcus faecium Modulates the Gut Microbiota of Broilers and Enhances Phosphorus Absorption and Utilization

Cited by 24 publications

References 51 publications

Intestinal Mucosal Immunity-Mediated Modulation of the Gut Microbiome by Oral Delivery of Enterococcus faecium Against Salmonella Enteritidis Pathogenesis in a Laying Hen Model

Intestinal Mucosal Immunity-Mediated Modulation of the Gut Microbiome by Oral Delivery of Enterococcus faecium Against Salmonella Enteritidis Pathogenesis in a Laying Hen Model

Gut microbiota is associated with bone mineral density

Progress on Gut Health Maintenance and Antibiotic Alternatives in Broiler Chicken Production

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