Enterohemorrhagic Escherichia coli O157:H7 Survival in an
            <i>In Vitro</i>
            Model of the Human Large Intestine and Interactions with Probiotic Yeasts and Resident Microbiota

Thévenot, Jonathan; Etienne‐Mesmin, Lucie; Denis, Sylvain; Chalancon, Sandrine; Alric, Monique; Livrelli, Valérie; Blanquet‐Diot, Stéphanie

doi:10.1128/aem.03303-12

Cited by 37 publications

(23 citation statements)

References 55 publications

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“…This would enhance the production of virulence genes, allowing the pathogen to establish a niche in the colon. An established microbial community may be robust enough to negate the effects of E. coli O157:H7 on microbial community structure by increasing the production of acetic acid while reducing that of butyric acid (24). The shift in short-chain fatty acid production in this study implies that the pathogen is now fully integrated into the microbial community and is influencing the microbial community to reduce acetate production and increase butyric acid production in order to allow the pathogen to produce virulence factors and find an ecological niche.…”

Section: Discussionmentioning

confidence: 99%

“…The function of in vitro gut environments has been tested by determining the microorganisms present (17,(21)(22)(23) and by monitoring fatty acid production (15,17,21,24) and enzymatic activity (17,21). For aquatic systems, such as surface water, wastewater, and groundwater, researchers have generally used synthetic formulas to recreate the aquatic chemistry found in those environments and to alleviate variability (18,(25)(26)(27)(28).…”

mentioning

confidence: 99%

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Linking Microbial Community Structure to Function in Representative Simulated Systems

Marcus

Wilder

Quazi

et al. 2013

Appl Environ Microbiol

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Pathogenic bacteria are generally studied as a single strain under ideal growing conditions, although these conditions are not the norm in the environments in which pathogens typically proliferate. In this investigation, a representative microbial community along with Escherichia coli O157:H7, a model pathogen, was studied in three environments in which such a pathogen could be found: a human colon, a septic tank, and groundwater. Each of these systems was built in the lab in order to retain the physical/ chemical and microbial complexity of the environments while maintaining control of the feed into the models. The microbial community in the colon was found to have a high percentage of bacteriodetes and firmicutes, while the septic tank and groundwater systems were composed mostly of proteobacteria. The introduction of E. coli O157:H7 into the simulated systems elicited a shift in the structures and phenotypic cell characteristics of the microbial communities. The fate and transport of the microbial community with E. coli O157:H7 were found to be significantly different from those of E. coli O157:H7 studied as a single isolate, suggesting that the behavior of the organism in the environment was different from that previously conceived. The findings in this study clearly suggest that to gain insight into the fate of pathogens, cells should be grown and analyzed under conditions simulating those of the environment in which the pathogens are present.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Linking Microbial Community Structure to Function in Representative Simulated Systems

Marcus

Wilder

Quazi

et al. 2013

Appl Environ Microbiol

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show abstract

“…It's the first one that has allowed the maintaining of anaerobiosis inside the fermentor by the sole metabolic activity of the microbiota and not by flushing with N 2 or CO 2 , as usually done in other colonic in vitro models. Up to date, ARCOL has been used to reproduce the colon of humans (Blanquet-Diot et al, 2012;Cordonnier et al, 2015;Thevenot et al, 2015;Thevenot et al, 2013), pre-ruminant calves (Gerard-Champod et al, 2010) and pigs.…”

Section: The Artificial Colon: Arcolmentioning

confidence: 99%

Can dynamicin vitrodigestion systems mimic the physiological reality?

Dupont

Alric

Blanquet‐Diot

et al. 2018

Critical Reviews in Food Science and Nutrition

243

128

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During the last decade, there has been a growing interest in understanding the fate of food during digestion in the gastrointestinal tract in order to strengthen the possible effects of food on human health. Ideally, food digestion should be studied in vivo on humans but this is not always ethically and financially possible. Therefore simple static in vitro digestion models mimicking the gastrointestinal tract have been proposed as alternatives to in vivo experiments but these models are quite basic and hardly recreate the complexity of the digestive tract. In contrast, dynamic models that allow pH regulation, flow of the food and injection in real time of digestive enzymes in the different compartments of the gastrointestinal tract are more promising to accurately mimic the digestive process. Most of the systems developed so far have been compared for their performances to in vivo data obtained on animals and/or humans. The objective of this article is to review the validation towards in vivo data of some of the dynamic digestion systems currently available in order to determine what aspects of food digestion they are able to mimic. Eight dynamic digestion systems are presented as well as their validation towards in vivo data. Advantages and limits of each simulator is discussed. This is the result of a cooperative international effort made by some of the scientists involved in Infogest, an international network on food digestion.

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“…In the three studies that mimicked human digestive conditions, S. cerevisiae CNCM I-3856 was implicated in having antagonistic effects on STEC including downregulating Stx expression and how the resident microbiota regulates infectivity [126][127][128][129].…”

Section: Probiotics And/or Prebiotics As Therapeutics To Combat Gastrmentioning

confidence: 99%

Prebiotics, Probiotics, and Bacterial Infections

Tam¹,

Land²,

Cheng³

2020

Prebiotics and Probiotics - Potential Benefits in Nutrition and Health

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Bacterial pathogens have developed exquisite virulence mechanisms to survive in the host cells. These virulence mechanisms help them bind and internalize into host cells, replicate, and evade the host immune response. The mammalian host itself has developed its own repertoire of weapons to prevent this from happening. One important component of host response in preventing infections in the gut lumen is the diverse commensal microbiota present. Dysbiosis of the gut microbiota has been implicated in the development of many gastrointestinal diseases. A potential therapeutic pathway to solve these diseases would be by providing probiotics and/or prebiotics to help stimulate growth of the beneficial commensal bacteria. Here, we will present evidence of commensal microbiota imbalance in the development of disease as well as potential therapies to restore gut harmony.

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Enterohemorrhagic Escherichia coli O157:H7 Survival in an In Vitro Model of the Human Large Intestine and Interactions with Probiotic Yeasts and Resident Microbiota

Cited by 37 publications

References 55 publications

Linking Microbial Community Structure to Function in Representative Simulated Systems

Linking Microbial Community Structure to Function in Representative Simulated Systems

Can dynamicin vitrodigestion systems mimic the physiological reality?

Prebiotics, Probiotics, and Bacterial Infections

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