Enteropathogens identified in cats entering a Florida animal shelter with normal feces or diarrhea

Sabshin, Stephanie J; Levy, Julie; Tupler, Tiffany; Tucker, Sylvia J; Greiner, Ellis C.; Leutenegger, Christian M.

doi:10.2460/javma.241.3.331

Cited by 101 publications

(103 citation statements)

References 46 publications

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“…Table 1). The lack of defined clinical symptoms in cats shedding one of the viruses supports prior studies reporting frequent enteric viral infections in healthy animals (Sabshin et al, 2012; Shan et al, 2011) and humans (Ayukekbong et al, 2011; Kapusinszky et al, 2012; Witsø et al, 2006). In order to determine whether these feline viruses may cause disease in a subset of animals (e.g.…”

Section: Discussionsupporting

confidence: 80%

Feline fecal virome reveals novel and prevalent enteric viruses

Mesquita

Nascimento

et al. 2014

Veterinary Microbiology

136

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Humans keep more than 80 million cats worldwide, ensuring frequent contacts with their viruses. Despite such interactions the enteric virome of cats remains poorly understood. We analyzed a fecal sample from a single healthy cat from Portugal using viral metagenomics and detected five eukaryotic viral genomes. These viruses included a novel picornavirus (proposed genus “Sakobuvirus”) and bocavirus (feline bocavirus 2), a variant of feline astrovirus 2 and sequence fragments of a highly divergent feline rotavirus and picobirnavirus. Feline sakobuvirus A represents the prototype species of a proposed new genus in the Picornaviridae family, distantly related to human salivirus and kobuvirus. Feline astroviruses (mamastrovirus 2) are the closest relatives of the classic human astroviruses (mamastrovirus 1), suggestive of past cross-species transmission. Presence of these viruses by PCR among Portuguese cats was detected in 13% (rotavirus), 7% (astrovirus), 6% (bocavirus), 4% (sakobuvirus), and 4% (picobirnavirus) of 55 feline fecal samples. Co-infections were frequent with 40% (4/10) of cats shedding more than one of these viruses. Our study provides an initial unbiased description of the feline fecal virome indicating a high level of asymptomatic infections. Availability of the genome sequences of these viruses will facilitate future tropism and disease association studies.

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Section: Discussionsupporting

confidence: 80%

Feline fecal virome reveals novel and prevalent enteric viruses

Mesquita

Nascimento

et al. 2014

Veterinary Microbiology

136

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“…We were also able to use the differential pan-Salmonella FRET-PCR to detect Salmonella in dog and cat feces from apparently healthy animals. Although we did not culture the feces from the dogs and cats and were therefore unable to precisely determine the sensitivity and specificity of the test when performed directly on fecal samples, our findings are comparable with other studies using PCR which found Salmonella in 6% and 2% of dogs [24] and cats [25] with normal feces, respectively. Further studies are underway in our laboratory to determine the sensitivity of the PCR in detecting DNA of Salmonella directly in tissue, fecal and environmental samples.…”

Section: Discussionsupporting

confidence: 74%

Detection of Salmonella spp. Using a Generic and Differential FRET-PCR

et al. 2013

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To facilitate the detection of Salmonella and to be able to rapidly and conveniently determine the species/subspecies present, we developed and tested a generic and differential FRET-PCR targeting their tetrathionate reductase response regulator gene. The differential pan-Salmonella FRET-PCR we developed successfully detected seven plasmids that contained partial sequences of S. bongori and the six S. enterica subspecies. The detection limit varied from ∼5 copies of target gene/per PCR reaction for S. enterica enterica to ∼200 for S. bongori. Melting curve analysis demonstrated a T m of ∼68°C for S. enterica enterica, ∼62.5°C for S. enterica houtenae and S. enterica diarizonae, ∼57°C for S. enterica indica, and ∼54°C for S. bongori, S. enterica salamae and S. enterica arizonae. The differential pan-Salmonella FRET-PCR also detected and determined the subspecies of 4 reference strains and 47 Salmonella isolated from clinically ill birds or pigs. Finally, we found it could directly detect and differentiate Salmonella in feline (5/50 positive; 10%; one S. enterica salamae and 4 S. enterica enterica) and canine feces (15/114 positive; 13.2%; all S. enterica enterica). The differential pan-Salmonella FRET-PCR failed to react with 96 non-Salmonella bacterial strains. Our experiments show the differential pan-Salmonella FRET-PCR we developed is a rapid, sensitive and specific method to detect and differentiate Salmonella.

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“…Vertical transmission has not been demonstrated (Foley et al, 1997). Persistently infected, asymptomatic carriers spread FCoV since most of these cats shed the virus for a period of months or years, either continuously or transiently (Foley et al, 1997;Cave et al, 2004;Dye et al, 2008;Kipar et al, 2010;Sabshin et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

A retrospective clinical and epidemiological study on feline coronavirus (FCoV) in cats in Istanbul, Turkey

Tekelioğlu¹,

Berriatua

Turan

et al. 2015

Preventive Veterinary Medicine

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The presence of antibodies to feline coronavirus (FCoV) and feline immunodeficiency virus (FIV), together with feline leukemia virus (FeLV) antigen was investigated in 169 ill household and stray cats attending a veterinary surgery in Istanbul in 2009-14. The estimated FCoV and FIV seroprevalence (95% confidence intervals) were 37% (30-45%) and 11% (6-16%), respectively and FeLV prevalence was 1% (0-3%). FCoV seroprevalence increased until 2 years of age, was highest in 2014 and among household cats living with other cats and with outdoor access, and was lower in FIV seropositive compared to seronegative cats. Symptoms typically associated with wet feline infectious peritonitis (FIP) including ascites, abdominal distention or pleural effusion, coupled in many cases with non-antibiotic responsive fever, were observed in 19% (32/169) of cats, and 75% (24/32) of these cats were FCoV seropositive. FCoV seropositivity was also associated with a high white blood cell count, high plasma globulin, low plasma albumin and low blood urea nitrogen. The percentage of FCoV seropositive and seronegative cats that died in spite of supportive veterinary treatment was 33% (21/63) and 12% (13/106), respectively. These results indicate that FCoV is widespread and has a severe clinical impact in cats from Istanbul. Moreover, the incidence of FCoV infections could be rising, and in the absence of effective vaccination cat owners need to be made aware of ways to minimize the spread of this virus.

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Enteropathogens identified in cats entering a Florida animal shelter with normal feces or diarrhea

Cited by 101 publications

References 46 publications

Feline fecal virome reveals novel and prevalent enteric viruses

Feline fecal virome reveals novel and prevalent enteric viruses

Detection of Salmonella spp. Using a Generic and Differential FRET-PCR

A retrospective clinical and epidemiological study on feline coronavirus (FCoV) in cats in Istanbul, Turkey

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