Enterotoxigenic Escherichia coli O153?H45 from an outbreak of diarrhoea in Spain

Escribano, Alicia; Ørskov, Ida; Ørskov, Frits; Borrás, R Gil

doi:10.1007/bf00190530

Cited by 9 publications

(7 citation statements)

References 13 publications

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“…A n ETEC strain of serotype 0153: K-: H45 was also isolated in another outbreak localized in Madrid, but in another hospital, in 1984. In previous studies, enterotoxigenic strains of serotype 0153:H45 have also been found to be the most common ETEC strains occurring in sporadic cases of infant diarrhoea in Chile (1) and in Spain (9), but they have only been isolated occasionally in other countries (12,16,20,21,22). Escribano et aL (21) reported that an STaproducing strain of serotype 0153: H45 was responsible for an outbreak of neonatal diarrhoea in 1984 in a Spanish hospital in the city of Valencia.…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, enterotoxigenic strains of serotype 0153:H45 have also been found to be the most common ETEC strains occurring in sporadic cases of infant diarrhoea in Chile (1) and in Spain (9), but they have only been isolated occasionally in other countries (12,16,20,21,22). Escribano et aL (21) reported that an STaproducing strain of serotype 0153: H45 was responsible for an outbreak of neonatal diarrhoea in 1984 in a Spanish hospital in the city of Valencia. The two enterotoxigenic strains 0153: K-: H45 detected in this study showed an identical antibiotic resistance pattern to the ETEC strain causing the diarrhoeal outbreak in Valencia (21) and to the majority of ETEC strains 0153:H45 isolated in Chile (1) from sporadic cases of infant diarrhoea.…”

Section: Discussionmentioning

confidence: 99%

“…Escribano et aL (21) reported that an STaproducing strain of serotype 0153: H45 was responsible for an outbreak of neonatal diarrhoea in 1984 in a Spanish hospital in the city of Valencia. The two enterotoxigenic strains 0153: K-: H45 detected in this study showed an identical antibiotic resistance pattern to the ETEC strain causing the diarrhoeal outbreak in Valencia (21) and to the majority of ETEC strains 0153:H45 isolated in Chile (1) from sporadic cases of infant diarrhoea. Our results suggest that, in addition to ETEC 0153: K-: H45, enterotoxigenic strains belonging to serogroup 0159 might be an important cause of diarrhoeal outbreaks in Spain.…”

Section: Discussionmentioning

confidence: 99%

“…ETEC strains are a major cause of diarrhoea in developing countries and are also the agent most frequently responsible for travelers' diarrhoea (1,29,32). In developed countries ETEC diarrhoea is rare (5,9,13), although occasional outbreaks have been reported (17,21). 1 Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

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Enterotoxigenic and necrotizing Escherichia coli in human diarrhoea in Spain

et al. 1992

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Enterotoxigenic Escherichia coli (ETEC) strains of serotype 0153: K-:H45 CFA/I+ STa+ were associated with two outbreaks of neonatal diarrhoea that occurred in two different hospitals of Madrid, in one of which several children died. Two other outbreaks were associated with ETEC strains of serotypes 0159: K-:H21 (LT+) and 0159: K-:H4 (LT+ STa+) without CFA/I and CFA/II colonization factors. Necrotizing E. coli (NTEC) strains of serotype 06:K13, producing the cytotoxic necrotizing factor CNF1 and alpha-haemolysin, were also associated with two outbreaks of neonatal diarrhoea that occurred in a hospital in Madrid and in a hospital in Talavera de la Reina. The results of the characterization of some ETEC and NTEC strains isolated from sporadic cases of diarrhoea are also discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enterotoxigenic and necrotizing Escherichia coli in human diarrhoea in Spain

et al. 1992

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“…13 in Valencia on the Mediterranean coast of Spain. However, the rate was higher than that observed in other studies carried out in Spain,7933 in other European countries6* l o and in North America.89…”

Section: Discussionmentioning

confidence: 99%

Enterotoxigenic Escherichia coli associated with infant diarrhoea in Galicia, north-western Spain

Blanco

González²,

Blanco³

et al. 1991

Journal of Medical Microbiology

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Summary.To assess the role of enterotoxigenic Escherichia coli (ETEC) in infantile diarrhoea, 482 children with diarrhoea and 103 healthy controls, from three localities of Galicia, northwestern Spain, were investigated between 1985 and 1988. Rotavirus (37.3%) and Salmonella spp. (1 2.8%) were the most common causal agents, followed by ETEC (3.973, Campylobacter jejuni (2*3%), Shigella spp. (0.9%) and Yersinia enterocolitica (0.5%). ETEC were significantly more frequently isolated from children with diarrhoea who were under 1 month of age (26.5%) than from older diarrhoeic children (2.2%) (p < 0.001) or from healthy children who were under 1 month of age (0%) (p < 0.05). Among children who harboured ETEC, five of the nine children under 1 month of age developed diarrhoea in hospital, whereas none of the 10 children over 1 month of age did so. Seventeen ETEC isolates produced heat-stable enterotoxin (STa) only, four produced only heat-labile enterotoxin (LT), and two produced both toxins. Colonisation factor antigens CFA/I and CFA/II were detected in 11 (55.0%) of the 20 ETEC isolates that remained enterotoxigenic after maintenance in the laboratory. Most ETEC isolates belonged to serotypes 0 1 53 : K -: H45 (nine STa' CFA/I + isolates), 027 : K -: H7 (three STa' isolates) or 0 6 : K15 : H16 (two LT' STa' CFA/II' isolates). Our results suggest that ETEC constitute an important cause of neonatal diarrhoea in this part of Spain.

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Serotypes and colonization factors of enterotoxigenic Escherichia coli isolated in various countries

et al. 1993

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One hundred and six enterotoxigenic E. coli (ETEC) isolated from many geographical areas were serotyped and investigated for the presence of colonization factor antigens CFA/I and CFA/II, the expression of mannose-resistant haemagglutination (MRHA) and the levels of surface hydrophobicity. CFA/I was found in 6 (17%) of 36 LT+STa+ strains and in 15 (54%) of 28 STa+ strains; CFA/II was found in 16 (44%) of 36 LT+STa+ strains. None of 42 LT+ strains showed CFA/I or CFA/II. CFA/I was found in ETEC of serotypes O63:K-:H-, O78:K80, O128:K67 and O153:K:H45, whereas CFA/II was found in serotypes O6:H-, O6:K15:H16 and O6:K?:H40. Of the 69 CFA/I- CFA/II- ETEC strains, 9 (13%) showed MRHA with some of the seven erythrocyte species used and 21 (30%) were hydrophobic. Among the 21 hydrophobic strains CFA-negative we have detected: (i) 6 LT+ strains of serogroup O25 negative for MRHA, (ii) 5 strains O159 (4 LT+ and 1 LT+ STa+) also negative for MRHA, and (iii) 3 STa+ strains of serotype O27:K-:H7 that haemagglutinated calf and sheep erythrocytes when grown on Minca-Is. The 106 ETEC strains belonged to 20 different O serogroups. However, 77 (73%) were of one of nine serogroups (O6, O8, O25, O27, O78, O148, O153, O159 and O167). E. coli strains belonging to O6 and O153 groups predominated among ETEC isolated in Spain, O159 strains in the Central African Republic, O25 and O148 strains in Japan, and O15 and O78 strains in India.

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Enterotoxigenic Escherichia coli O153?H45 from an outbreak of diarrhoea in Spain

Abstract: Enterotoxigenic Escherichia coli (ETEC) strains belonging to a characteristic serobiotype, O153:H45 ST+, were isolated from an outbreak of neonatal diarrhoea in Valencia, Spain. The restriction of the geographical location of this clone to South America and Spain is discussed.

Cited by 9 publications

References 13 publications

Enterotoxigenic and necrotizing Escherichia coli in human diarrhoea in Spain

Enterotoxigenic and necrotizing Escherichia coli in human diarrhoea in Spain

Enterotoxigenic Escherichia coli associated with infant diarrhoea in Galicia, north-western Spain

Serotypes and colonization factors of enterotoxigenic Escherichia coli isolated in various countries

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