Enterotoxigenic properties of Staphylococcus aureus isolated from goats' milk cheese

Akineden, Ömer; Hassan, Abdulwahed Ahmed; Schneider, Elisabeth; Usleber, Ewald

doi:10.1016/j.ijfoodmicro.2008.03.027

Cited by 110 publications

(86 citation statements)

References 36 publications

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“…In agreement with previous studies, we found that enterotoxigenic CM isolates mainly harbored the sec gene (Akineden et al 2008;Lyra et al 2013;Scherrer et al 2004). None of our CM isolates carried the seb gene.…”

Section: Detection Of Se and Tst Genessupporting

confidence: 93%

“…The co-detection of sec and tst genes observed in our study is consistent with other studies of S. aureus from mammary secretions of dairy animals and from bulk milk (Akineden et al 2008;Jørgensen et al 2005;Orden et al 1992;Scherrer et al 2004). …”

Section: Detection Of Se and Tst Genessupporting

confidence: 93%

“…DNA amplification was performed in a Perkin-Elemer GeneAmp 2400 thermocycler (Applied Biosystems, UK). The reaction and amplification conditions used for the PCR assays were those described by Akineden et al (2008).…”

Section: Detection Of Enterotoxigenic S Aureus Strainsmentioning

confidence: 99%

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Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

Pexara

Σολωμάκος

Sergelidis

et al. 2015

Dairy Sci. & Technol.

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Ovine (n=140) and caprine (n=35) raw bulk tank milk samples from farms in central Greece were examined for the occurrence of enterotoxigenic Staphylococcus aureus. The S. aureus isolates were screened for staphylococcal enterotoxin (SE) production, the presence of enterotoxin genes, antibiotic resistance (AR), and methicillin resistance. S. aureus was isolated from 24.3% and 31.4% of ovine and caprine milk samples, respectively. Among the S. aureus isolates of ovine milk (n=34) and caprine (n=11) milk, the enterotoxigenic (SEA-SED) isolates were 21 (61.8%) and 7 (63.6%) for the ovine and caprine milk, respectively. Most toxigenic isolates harbored more than one toxin gene and a total of 11 distinct toxinotypes were detected. The most frequent toxin-gene combinations were "sec, tst" (8 isolates), "seb, seg, sei, tst" (4), "seb, seg, sei" (3), and "seb" (3). Six isolates displayed multiple AR towards up to five antimicrobials. Among ovine milk isolates, the highest resistance frequency was observed towards erythromycin (11.8% of the isolates) and tetracycline (8.8%). Among caprine milk isolates, the most frequent resistance was observed towards erythromycin (18.2%). One methicillin-resistant S. aureus (MRSA) isolate was detected in an ovine milk sample and belonged to spa type t4038. This spa type

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Section: Detection Of Se and Tst Genessupporting

confidence: 93%

Section: Detection Of Se and Tst Genessupporting

confidence: 93%

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Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

Pexara

Σολωμάκος

Sergelidis

et al. 2015

Dairy Sci. & Technol.

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“…The higher prevalence 75% and 46% has been obtained by [36,37] respectively. The lower prevalence (10%, 9.5%, and 7.7%) were observed in different countries in the studies [38][39][40] in Iran, turkey and European countries respectively. The observed variation in prevalence of S. aureus especially in milk products may be reflect the level of sanitary measures in milk manufacture or due to the differences in technological methods in cheese manufacture [35].…”

Section: Discussionmentioning

confidence: 87%

Prevalence and Multiplex PCR for Enterotoxin Genes of Staphylococcus aureus Isolates from Subclinical Mastitis and Kareish Cheese

Mousa¹,

Abdeen²,

Hussein³

et al. 2017

J Infect Dis Preve Med

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Aim: Staphylococcus aureus was categorized as a contagious pathogen incriminated in subclinical mastitis as well as in dairy products. From public health view, this organism causing food poisoning outbreaks via contamination of food products with its toxins. These studies highlight the prevalence of staphylococcus aureus among subclinical mastitic cases as well as cheese samples and the dominant enterotoxigenic genes.Methodology: Examination of 100 samples (50 from subclinical mastitis, and 50 kareish cheese) from Sadat city, Menoufia province. Results and interpretation:Bacteriological culturing on selective and specific medium revealed that 26.7% and 30% in subclinical mastitis and kareish cheese respectively were due to S. aureus. Furthermore, multiplex PCR proved to be efficient technique for detection of different enterotoxins genes. The sea, seb and see genes were the most prevalent genes among the tested S. aureus isolates. Although, no detection of sec and sed genes were observed. Conclusion:It can be concluded that molecular characterization of S. aureus and its enterotoxins genes will be beneficial in designing control and preventive measures of S. aureus infection in human and animals.

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“…The highest microbial counts of S. aureus in cheese samples are determined as 10 5 CFU/g for production of enterotoxins by the official regulations (Alomar et al, 2008). Although pasteurization of milk, fermentation and ripening steps retard S. aureus growth, it is important to detect and quantify this pathogenic bacterium in cheese (Akineden et al, 2008). By this qPCR method species specific detection was performed under the described conditions.…”

Section: Quantification Of S Aureus In Naturally Contaminated Cheesementioning

confidence: 99%

Quantification of Staphylococcus aureus in white cheese by the improved DNA extraction strategy combined with TaqMan and LNA probe-based qPCR

Kadiroğlu

Korel

Ceylan

2014

Journal of Microbiological Methods

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Enterotoxigenic properties of Staphylococcus aureus isolated from goats' milk cheese

Cited by 110 publications

References 36 publications

Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

Prevalence and Multiplex PCR for Enterotoxin Genes of Staphylococcus aureus Isolates from Subclinical Mastitis and Kareish Cheese

Quantification of Staphylococcus aureus in white cheese by the improved DNA extraction strategy combined with TaqMan and LNA probe-based qPCR

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