Enterotoxin and toxic shock syndrome toxin‐one production by staphylococci isolated from mastitis in sheep

Orden, José A.; Hierro, Eva; Blanco, Maria Emilia; Quiteria, José Antonio Ruiz Santa; Gómez-Lucı́a, Esperanza; Fuente, Ricardo de la

doi:10.1111/j.1699-0463.1992.tb00851.x

Cited by 17 publications

(9 citation statements)

References 18 publications

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“…The co-detection of sec and tst genes observed in our study is consistent with other studies of S. aureus from mammary secretions of dairy animals and from bulk milk (Akineden et al 2008;Jørgensen et al 2005;Orden et al 1992;Scherrer et al 2004). …”

Section: Detection Of Se and Tst Genessupporting

confidence: 92%

“…It should be noted, however, that since only one isolate per S. aureus-positive bulk tank milk sample was further characterized in our study, it is quite likely that more than one S. aureus genotype could have been present in the respective S. aureus-positive bulk tank milk samples. According to literature data, SEC appears to be the predominant toxin type detected in S. aureus isolated from OM and particularly from mastitic OM (Orden et al 1992). It has been proposed that considerable geographical variation exists in the distribution of enterotoxigenic S. aureus (Jørgensen et al 2005).…”

Section: Detection Of Enterotoxigenic S Aureusmentioning

confidence: 99%

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Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

Pexara

Σολωμάκος

Sergelidis

et al. 2015

Dairy Sci. & Technol.

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Ovine (n=140) and caprine (n=35) raw bulk tank milk samples from farms in central Greece were examined for the occurrence of enterotoxigenic Staphylococcus aureus. The S. aureus isolates were screened for staphylococcal enterotoxin (SE) production, the presence of enterotoxin genes, antibiotic resistance (AR), and methicillin resistance. S. aureus was isolated from 24.3% and 31.4% of ovine and caprine milk samples, respectively. Among the S. aureus isolates of ovine milk (n=34) and caprine (n=11) milk, the enterotoxigenic (SEA-SED) isolates were 21 (61.8%) and 7 (63.6%) for the ovine and caprine milk, respectively. Most toxigenic isolates harbored more than one toxin gene and a total of 11 distinct toxinotypes were detected. The most frequent toxin-gene combinations were "sec, tst" (8 isolates), "seb, seg, sei, tst" (4), "seb, seg, sei" (3), and "seb" (3). Six isolates displayed multiple AR towards up to five antimicrobials. Among ovine milk isolates, the highest resistance frequency was observed towards erythromycin (11.8% of the isolates) and tetracycline (8.8%). Among caprine milk isolates, the most frequent resistance was observed towards erythromycin (18.2%). One methicillin-resistant S. aureus (MRSA) isolate was detected in an ovine milk sample and belonged to spa type t4038. This spa type

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Section: Detection Of Se and Tst Genessupporting

confidence: 92%

Section: Detection Of Enterotoxigenic S Aureusmentioning

confidence: 99%

Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

Pexara

Σολωμάκος

Sergelidis

et al. 2015

Dairy Sci. & Technol.

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“…It was interesting that all SEC producers of the present study were TSST-1 positive and all SED producers were SEJ positive. A comparable relationship between the detection of SEC and TSST-1 has been reported in the literature (11,27,32,40,51). The enterotoxins D and J are encoded by a plasmid and separated from each other by an intergenic region (50).…”

Section: Discussionsupporting

confidence: 81%

Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis

Akineden

Annemüller

Hassan

et al. 2001

Clin Diagn Lab Immunol

194

143

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In the present study, 103 Staphylococcus aureus strains isolated from milk samples from 60 cows with mastitis from eight different farms in seven different locations in one region of Germany were compared pheno-and genotypically and by identification of various toxins. On the basis of culture and hemolytic properties and by determination of the tube coagulase reaction, all of the isolates could be identified as S. aureus. This could be confirmed by PCR amplification of species-specific parts of the gene encoding the 23S rRNA. In addition, all of the S. aureus isolates harbored the genes encoding staphylococcal coagulase and clumping factor and the genes encoding the X region and the immunoglobulin G binding region of protein A. These four genes displayed size polymorphisms. By PCR amplification, the genes for the toxins staphylococcal enterotoxin A (SEA), SEC, SED, SEG, SEI, SEJ, and TSST-1 but not those for SEB, SEE, SEH, and the exfoliative toxins ETA and ETB could be detected. To analyze the epidemiological relationships, the isolates were subjected to DNA fingerprinting by macrorestriction analysis of their chromosomal DNAs. According to the observed gene polymorphisms, the toxin patterns, and the information given by macrorestriction analysis of the isolates by pulsedfield gel electrophoresis, a limited number of clones seemed to be responsible for the cases of bovine mastitis on the various farms.

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“…Unlike the situation for S. aureus, only limited and conflicting data are available regarding enterotoxin production in non-S. aureus staphylococcal species (10,23,25,46,52,57,63,65). One of these species, the coagulase-positive S. intermedius, is the predominant staphylococcal species isolated from healthy and diseased dogs and has been isolated from a number of other carnivores, horses, and birds (16,36,54).…”

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confidence: 99%

Enterotoxigenic Potential ofStaphylococcus intermedius

Becker

Keller

Eiff

et al. 2001

Appl Environ Microbiol

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Staphylococcal food poisoning (SFP) caused by enterotoxigenic staphylococci is one of the main food-borne diseases. In contrast to Staphylococcus aureus, a systematic screening for the enterotoxins has not yet been performed on the genomic level for the coagulase-positive species S. intermedius. Therefore, the enterotoxigenic potential of 281 different veterinary (canine, n ‫؍‬ 247; equine, n ‫؍‬ 23; feline, n ‫؍‬ 9; other, n ‫؍‬ 2) and 11 human isolates of S. intermedius was tested by using a multiplex PCR DNA-enzyme immunoassay system targeting the staphylococcal enterotoxin genes sea, seb, sec, sed, and see. Molecular results were compared by in vitro testing of enterotoxin production by two immunoassays. A total of 33 (11.3%) S. intermedius isolates, including 31 (12.6%) canine isolates, 1 equine isolate, and 1 human isolate, tested positive for the sec gene. In vitro production of the respective enterotoxins was detected in 30 (90.9%) of these isolates by using immunological tests. In contrast, none of 65 veterinary specimen-derived isolates additionally tested and comprising 13 (sub)species of coagulase-negative staphylococci were found to be enterotoxigenic. This study shows on both molecular and immunological levels that a substantial number of S. intermedius isolates harbor the potential for enterotoxin production. Since evidence for noninvasive zoonotic transmission of S. intermedius from animal hosts to humans has been documented, an enterotoxigenic role of this microorganism in SFP via contamination of food products may be assumed.

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Enterotoxin and toxic shock syndrome toxin‐one production by staphylococci isolated from mastitis in sheep

Cited by 17 publications

References 18 publications

Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

Occurrence and antibiotic resistance of enterotoxigenic Staphylococcus aureus in raw ovine and caprine milk in Greece

Toxin Genes and Other Characteristics of Staphylococcus aureus Isolates from Milk of Cows with Mastitis

Enterotoxigenic Potential ofStaphylococcus intermedius

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