Enteroviral Capsid Protein VP1 Is Present in Myocardial Tissues From Some Patients With Myocarditis or Dilated Cardiomyopathy

Li, Yanwen; Bourlet, Thomas; Andréoletti, Laurent; Mosnier, Jean‐François; Peng, Tianqing; Yang, Yifei; Archard, L. C.; Pozzetto, Bruno; Zhang, Hongyi

doi:10.1161/01.cir.101.3.231

Cited by 129 publications

(91 citation statements)

References 15 publications

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“…13 Although enteroviruses have classically been identified as the prime viral agent, new techniques to extract viral genome from myocardium with polymerase chain reaction techniques have in both children and adults revealed previously unrecognized viruses such as adenovirus, parvovirus B19, human herpesvirus 6, hepatitis C, Epstein-Barr virus, and cytomegalovirus. 2,3,[14][15][16][17][18][19] Interestingly, the pattern of identified viral pathogens in myocarditis has evolved over the last 20 years from enteroviruses and adenoviruses to primarily parvovirus and herpesvirus 6. Endomyocardial fibroelastosis, a once frequent cause of infantile dilated cardiomyopathy that is now rarely seen, was linked to the mumps virus via viral polymerase chain reaction analysis of archived pathological sample, suggesting that its reduced prevalence might be attributed to immunization.…”

mentioning

confidence: 99%

Diagnosis and Treatment of Myocarditis in Children in the Current Era

2014

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mentioning

confidence: 99%

Diagnosis and Treatment of Myocarditis in Children in the Current Era

2014

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“…Myocarditis scoring was done as described in materials and methods. *P 0.05 compared with CVB3-H3, P 0.05 compared with Day 3. culture in the tissue (Li et al, 2000;McCarthy et al, 2000;Seong et al, 2001). Moreover, the time window for the detection of inflammation after a virus infection, based on infectious-immune hypothesis (Kawai et al, 1999;Feldman et al, 2000;Jung et al, 2003), remains to be defined.…”

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confidence: 99%

Myocardial injury occurs earlier than myocardial inflammation in acute experimental viral myocarditis

Lim

Shin²,

Choe³

et al. 2005

Exp Mol Med

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“…The methods applied for immunohistochemical stainings and antibodies used have been described in detail elsewhere (8,11). For visualization of all antibodies, a labeled streptavidin-biotin technique was used; 3-amino-9-ethylcarbazol was applied as chromogen.…”

Section: Postmortem Samplesmentioning

confidence: 99%

Role of Virus-Induced Myocardial Affections in Sudden Infant Death Syndrome: A Prospective Postmortem Study

et al. 2004

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The cause of sudden infant death syndrome (SIDS) is an unresolved problem of high relevance. Previous studies indicate a role of infections. In our prospective study, we investigated the frequency of virus-induced myocardial affections in SIDS. Postmortem samples from SIDS victims and control subjects were investigated prospectively. Pediatric cases of unnatural death served as controls. Samples were studied for enteroviruses, adenoviruses, parvovirus B19, and Epstein-Barr virus applying PCR. Immunohistochemical investigations for inflammatory cells, the necrosis marker C5b-9 (m) complement complex, and the enteroviral capsid protein VP1 were performed. Overall, 62 SIDS victims were studied. As controls, 11 infants were enrolled. Enteroviruses were detected in 14 (22.5%), adenoviruses in 2 (3.2%), Epstein-Barr viruses in 3 (4.8%), and parvovirus B19 in 7 (11.2%) cases of SIDS. Control group samples were completely virus negative. Compared with controls, immunohistochemical investigations partially revealed a significant increase in the number of T lymphocytes in SIDS myocardial samples (p Ͻ 0.05). Furthermore, cases with elevated numbers of leukocytes and macrophages, microfocal C5b-9 (m) ϩ necroses, and enteroviral VP1 capsid protein within the myocardium were detected. Applying a comprehensive combination of molecular and immunohistochemical techniques, our results demonstrate a clearly higher prevalence of viral myocardial affections in SIDS. Our results emphasize the importance of PCR-based diagnosis of viral myocardial affections. We suggest preliminary criteria for cellular immunohistochemical diagnosis of viral myocardial affections derived from our findings. The diagnosis of sudden infant death syndrome (SIDS) is established by comprehensive exclusion of all other possible causes of death in the age group. Myocarditis is a widely known explanation for sudden death in cases of suspected SIDS as well as in older children (1). Recent clinical studies and anecdotal communications reported on different viruses in such cases applying molecular techniques to detect the genome sequences of enteroviruses (EV) (2), adenoviruses (AV) (3), Epstein-Barr virus (EBV) (4), and parvovirus B19 (PVB19) (5) in clinical and autopsy samples, respectively. Until recently, a maximum percentage of up to 17% of cases of sudden unexpected death in infancy showing-if at all-histologic hints of viral myocardial affections was assumed (6). The aim of our study was to determine the incidence of virus-induced myocardial affections in cases of SIDS with modern immunohistochemical and molecular pathologic methods. METHODS

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Enteroviral Capsid Protein VP1 Is Present in Myocardial Tissues From Some Patients With Myocarditis or Dilated Cardiomyopathy

Cited by 129 publications

References 15 publications

Diagnosis and Treatment of Myocarditis in Children in the Current Era

Diagnosis and Treatment of Myocarditis in Children in the Current Era

Myocardial injury occurs earlier than myocardial inflammation in acute experimental viral myocarditis

Role of Virus-Induced Myocardial Affections in Sudden Infant Death Syndrome: A Prospective Postmortem Study

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