Entomopathogenic fungi-mediated biological control of the red palm weevil Rhynchophorus ferrugineus

Yang, Tzu-Hao; Wu, Li-Hsin; Liao, Chung-Ta; Li, Dongwei; Shin, Tae Young; Nai, Yu‐Shin

doi:10.1016/j.aspen.2023.102037

Cited by 9 publications

(4 citation statements)

References 13 publications

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“…Prior to use under field conditions, the most important aspect is to identify highly virulent isolates through laboratory screening bioassays [61]. Results of our screening assay showed that all isolates were pathogenic to R. ferrugineus and further supported the findings of Sun et al and Yang et al [61,62] who worked with different B. bassiana and M. anisopliae isolates. Although, R. ferrugineus was susceptible to all isolates, the isolates varied in their virulence.…”

Section: Discussionsupporting

confidence: 82%

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Efficacy and Persistence of Entomopathogenic Fungi against Rhynchophorus ferrugineus on Date Palm: Host to Host Transmission

Alwaneen,

Wakil,

Kavallieratos

et al. 2024

Agronomy

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The red palm weevil (RPW), Rhynchophorus ferrugineus (Olivier) (Coleoptera: Dryophthoridae), is a destructive and voracious pest of palm species worldwide. Due to environmental and regulatory concerns, ecologically safe alternatives to synthetic chemical insecticides are needed to manage this cryptic insect species. Entomopathogenic fungi have the potential to manage this pest. The scope of management and effectiveness can be improved by direct control or horizontal transmission of entomopathogenic fungal isolates. We tested in the laboratory the virulence and pathogenicity of fifteen different entomopathogenic fungal isolates belonging to the following species: Beauveria bassiana, Metarhizium anisopliae, Beauveria brongniartii and Purpureocillium lilacinum. All fungal isolates were found virulent against larvae (14.9 ± 1.06 to 81.5 ± 1.48% mortality) and adults (5.6 ± 1.12 to 51.7 ± 1.51% mortality) at 12 d post-treatment. From a screening bioassay, five M. anisopliae (WG-08, WG-09) and B. bassiana (WG-23, WG-24, WG-25) isolates were tested for their concentration response mortality against larvae and adults after 7, 14 and 21 days (d) of treatment. Mortality was found positively correlated with concentration and time. At 21 d of treatment, WG-23 and WG-25 1 × 108 conidia/mL resulted in 100% mortality against larvae while only WG-25 1 × 109 conidia/mL caused 100% mortality of adults. Along with mortality, all the potential isolates have strong ovicidal effects that reduced 81.49% at 1 × 108 conidia/mL. The horizontal transmission bioassay indicated that the infected adults transmitted the disease to healthy individuals. Horizontal transmission of fungi from infected to non-infected adults not only caused significant mortality but also had a serious sublethal impact on insect development and fitness including reduced number of eggs/d fecundity, egg viability and neonate survival. Isolate WG-25 reduced oviposition (0.5 eggs/d), fecundity (11.7 eggs/female), egg viability (11.6%) along with larval survival 25.9% when infected male mated with normal female. In semi-field trials, all fungal isolates reduced survival of larvae found inside the palms and ultimately reduced infestations over a period of two months. The results of this study indicate that entomopathogenic fungi should be further tested for sustainable and efficient control of RPW in date palm production systems.

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Section: Discussionsupporting

confidence: 82%

“…Several studies have confirmed the efficacy of various isolates of EPF against different developmental stages of R. ferrugineus [28,61,62,72,83,84]. In the field, larvae due to their cryptic nature [85], are difficult to infect with EPF.…”

Section: Discussionmentioning

confidence: 97%

Efficacy and Persistence of Entomopathogenic Fungi against Rhynchophorus ferrugineus on Date Palm: Host to Host Transmission

Alwaneen,

Wakil,

Kavallieratos

et al. 2024

Agronomy

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“…Conversely, the Bv065 strain was not significantly effective in causing lethal effects to the weevil species R. palmarum , which has shown susceptibility to other B. bassiana strains as reported in other studies (León-Martínez et al 2019 ; Dalbon et al 2024 ). Also, B. bassiana has been effective for controlling other palm weevil species like R. ferrugineus (Dembilio et al 2010 ; Yang et al 2023 ). Although the Bv065 strain may not cause significant lethal effects on palmarum, there may be sublethal effects of B. bassiana infection as suggested by previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…In the realm of integrated pest management, entomopathogenic fungi serve as pivotal biological control agents, particularly in tropical agricultural settings (McGuire and Northfield 2020 ). Notably, Beauveria and Metarhizium species have demonstrated their effectiveness as biological control agents against a range of agriculturally significant pests, including ticks (Sullivan et al 2022 a), whiteflies (Barra-Bucarei et al 2020 ; Sani et al 2020 ), citrus psyllids (Maluta et al 2022 ) and red palm weevils (Yang et al 2023 ). Their broad-spectrum efficacy encompasses all developmental stages of these pests, making them essential components of integrated pest management strategies.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a native strain of the entomopathogenic fungus Beauveria bassiana for the control of the palm weevil Dynamis borassi (Coleoptera: Curculionidae) in the neotropics

Gutiérrez,

Alarcón,

Ortiz

et al. 2024

World J Microbiol Biotechnol

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This study aimed to isolate and characterize a native strain of Beauveria bassiana, coded as Bv065, showcasing its potential as a biological control agent targeting the palm weevil Dynamis borassi. Originating from a naturally infected D. borassi specimen collected in southwestern Colombia, the fungus underwent molecular identification and was identified as B. bassiana, exhibiting high sequence similarity with known reference strains. The physiological characterization revealed that Bv065 thrived within a temperature range of 25 to 30 °C and a pH range of 6 to 9. Moreover, the key carbon sources that allow optimal growth of the strain were identified through metabolic profiling, including sucrose, D-mannose, and γ-amino-butyric acid. These findings offer strategic insights for scalability and formulation methodologies. Additionally, enzymatic analyses unveiled robust protease activity within Bv065, crucial for catalysing insect cuticle degradation and facilitating host penetration, thus accentuating its entomopathogenic potential. Subsequent evaluations exposed Bv065’s pathogenicity against D. borassi, causing significant mortality within nine days of exposure, albeit exhibiting limited effectiveness against Rhynchophorus palmarum. This study underscores the importance of understanding optimal growth conditions and metabolic preferences of B. bassiana strains for developing effective biopesticides. The findings suggest Bv065 as a promising candidate for integrated pest management strategies in neotropical regions, particularly for controlling palm weevil infestations in coconut and peach palm cultivation. Future research avenues include refining mass production methodologies, formulating novel delivery systems, and conducting comprehensive field efficacy trials to unlock the full potential of Bv065 in fostering sustainable pest management practices. Overall, this study contributes to the growing body of knowledge on entomopathogenic fungi and their pivotal role in biological control, offering nuanced perspectives on eco-friendly alternatives to conventional insecticidal interventions.

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Entomopathogenic fungi in crops protection with an emphasis on bioactive metabolites and biological activities

Shahbaz,

Palaniveloo,

Tan

et al. 2024

World J Microbiol Biotechnol

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Entomopathogenic fungi-mediated biological control of the red palm weevil Rhynchophorus ferrugineus

Cited by 9 publications

References 13 publications

Efficacy and Persistence of Entomopathogenic Fungi against Rhynchophorus ferrugineus on Date Palm: Host to Host Transmission

Efficacy and Persistence of Entomopathogenic Fungi against Rhynchophorus ferrugineus on Date Palm: Host to Host Transmission

Isolation and characterization of a native strain of the entomopathogenic fungus Beauveria bassiana for the control of the palm weevil Dynamis borassi (Coleoptera: Curculionidae) in the neotropics

Entomopathogenic fungi in crops protection with an emphasis on bioactive metabolites and biological activities

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