Entomopathogenic Fungus-Related Priming Defense Mechanisms in Cucurbits Impact Spodoptera littoralis (Boisduval) Fitness

García-Espinoza, Fabián; Rosal, María José García del; Quesada‐Moraga, Enrique; Yousef-Yousef, Meelad

doi:10.1128/aem.00940-23

Cited by 15 publications

(12 citation statements)

References 115 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results suggest that D. hansenii, with other microorganisms, could induce both ISR and SAR. Our results agree with the previous ones recently published by García-Espinoza et al [10], where the inoculation of cucumber and melon plants with the entomopathogenic fungus (EPF) Metarhizium brunneum Petch strain EAMa 01/58-Su induced the expression of several genes related to ET synthesis and transduction pathway, as well as PAL and LOX genes related to SA and JA synthesis. This relative expression enhancement was correlated with lethal and sublethal effects on Spodoptera littoralis.…”

Section: Discussionsupporting

confidence: 93%

“…In this work, we take a further step towards optimizing the use of yeasts as growth promoters and for enhancing plant nutrition. Although, both D. hansenii and H. polymorpha showed a remarkable ability to induce Fe deficiency responses, this study specifically Locally, some rhizosphere microorganisms can induce Fe deficiency responses, contributing to an improvement of plant nutrition and, as consequence, promoting plant growth and alleviating Fe chlorosis [1,2,10,11,26]. Created with BioRender.com.…”

Section: Introductionmentioning

confidence: 98%

“…Furthermore, both D. hansenii and H. polymorpha fostered the development of subapical root hairs, a characteristic morphological response to Fe deficiency [2] (Figure 1). Locally, some rhizosphere microorganisms can induce Fe deficiency responses, contributing to an improvement of plant nutrition and, as consequence, promoting plant growth and alleviating Fe chlorosis [1,2,10,11,26]. Created with BioRender.com.…”

Section: Introductionmentioning

confidence: 99%

“…Rhizosphere microorganisms trigger induced resistance through long-distance signals transported by the vascular system. Additionally, airborne signals can systemically amplify plant defense mechanisms against pathogen infections or infestations by herbivores in induced plant tissues, leading to notably reduced damage.Locally, some rhizosphere microorganisms can induce Fe deficiency responses, contributing to an improvement of plant nutrition and, as consequence, promoting plant growth and alleviating Fe chlorosis[1,2,10,11,26]. Created with BioRender.com.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Exploring the Role of Debaryomyces hansenii as Biofertilizer in Iron-Deficient Environments to Enhance Plant Nutrition and Crop Production Sustainability

Sevillano-Caño,

García,

Córdoba-Galván

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

The European “Green Deal” policies are shifting toward more sustainable and environmentally conscious agricultural practices, reducing the use of chemical fertilizer and pesticides. This implies exploring alternative strategies. One promising alternative to improve plant nutrition and reinforce plant defenses is the use of beneficial microorganisms in the rhizosphere, such as “Plant-growth-promoting rhizobacteria and fungi”. Despite the great abundance of iron (Fe) in the Earth’s crust, its poor solubility in calcareous soil makes Fe deficiency a major agricultural issue worldwide. Among plant promoting microorganisms, the yeast Debaryomyces hansenii has been very recently incorporated, for its ability to induce morphological and physiological key responses to Fe deficiency in plants, under hydroponic culture conditions. The present work takes it a step further and explores the potential of D. hansenii to improve plant nutrition and stimulate growth in cucumber plants grown in calcareous soil, where ferric chlorosis is common. Additionally, the study examines D. hansenii’s ability to induce systemic resistance (ISR) through a comparative relative expression study by qRT-PCR of ethylene (ET) biosynthesis (ACO1), or ET signaling (EIN2 and EIN3), and salicylic acid (SA) biosynthesis (PAL)-related genes. The results mark a significant milestone since D. hansenii not only enhances nutrient uptake and stimulates plant growth and flower development but could also amplify induced systemic resistance (ISR). Although there is still much work ahead, these findings make D. hansenii a promising candidate to be used for sustainable and environmentally friendly integrated crop management.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Exploring the Role of Debaryomyces hansenii as Biofertilizer in Iron-Deficient Environments to Enhance Plant Nutrition and Crop Production Sustainability

Sevillano-Caño,

García,

Córdoba-Galván

et al. 2024

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The relative expression levels of resistance genes were determined using qPCR employing specific primers. 43 Total DNA in each treatment was extracted and the specific primers employed in qPCR are listed in Table S1.…”

Section: In Vitro Evaluation Of Plant Disease Resistance Induced By F...mentioning

confidence: 99%

Metarhizium rileyi with broad‐spectrum insecticidal ability confers resistance against phytopathogens and insect pests as a phytoendophyte

Zhang,

Sui,

Tian

et al. 2024

Pest Management Science

View full text Add to dashboard Cite

BACKGROUNDEntomophagous fungi (EPF) not only directly kill insect pests, but also colonise plants and improve their resistance against pests. However, most previous research has focused on Beauveria bassiana and Metarhizium anisopliae, and there are few reports on whether other EPF can enhance resistance against pests via endogenous colonization. Herein, an EPF strain was isolated from diseased larvae of Spodoptera litura in a soybean field, and subjected to genome‐wide sequencing at the chromosomal level. The pathogenicity of the isolate toward various pest insects was evaluated, and the ability to colonize plants and induce resistance against phytopathogens and insect pests of were tested.RESULTSThe purified isolate was identified as M. rileyi and designated MrS1Gz1–1. Biological assays revealed its strong pathogenicity toward five insect pests belonging to Lepidoptera and Hemiptera. Furthermore, the strain inhibited the growth of soil‐borne plant disease caused by Sclerotinia sclerotiorum in vitro. It colonized plants as an endophyte via soil application, thereby inducing plant resistance‐related genes against phytopathogen infection, and it disrupted the feeding selectivity of S. litura larvae.CONCLUSIONM. rileyi MrS1Gz1–1 has potential as a broad‐spectrum microbial control agent that can induce resistance against phytopathogens and insect pests feeding as an endotype. The complete genome provides a valuable resource for exploring host interactions.

show abstract

Novel report on soil infection with Metarhizium rileyi against soil‐dwelling life stages of insect pests

Vivekanandhan,

Kamaraj,

Alharbi

et al. 2024

J Basic Microbiol

View full text Add to dashboard Cite

Entomopathogenic fungi are the most effective control remedy against a wide range of medical and agricultural important pests. The present study aimed to isolate, identify, and assess the virulence of Metarhizium rileyi against Spodoptera litura and Spodoptera frugiperda pupae under soil conditions. The biotechnological methods were used to identify the isolate as M. rileyi. The fungal conidial pathogenicity (2.0 × 107, 2.0 × 108, 2.0 × 109, 2.0 × 1010, and 2.0 × 1011 conidia/mL−1) was tested against prepupae of S. litura and S. frugiperda at 3, 6, 9, and 12 days after treatments. Additionally, the artificial soil‐conidial assay was performed on a nontarget species earthworm Eudrilus eugeniae, using M. rileyi conidia. The present results showed that the M. rileyi caused significant mortality rates in S. litura pupae (61‐90%), and S. litura pupae were more susceptible than S. frugiperda pupae (46%–73%) at 12 day posttreatment. The LC50 and LC90 of M. rileyi against S. litura, were 3.4 × 1014–9.9 × 1017 conidia/mL−1 and 6.6 × 105–4.6 × 1014 conidia/mL−1 in S. frugiperda, respectively. The conidia of M. rileyi did not exhibit any sublethal effect on the adult stage of E. eugeniae, and Artemia salina following a 12‐day treatment period. Moreover, in the histopathological evaluation no discernible harm was observed in the gut tissues of E. eugeniae, including the lumen and epithelial cells, as well as the muscles, setae, nucleus, mitochondria, and coelom. The present findings provide clear evidence that M. rileyi fungal conidia can be used as the foundation for the development of effective bio‐insecticides to combat the pupae of S. litura and S. frugiperda agricultural pests.

show abstract

Entomopathogenic Fungus-Related Priming Defense Mechanisms in Cucurbits Impact Spodoptera littoralis (Boisduval) Fitness

Abstract: Entomopathogenic fungi are multipurpose microorganisms with direct and indirect effects on insect pests. Also, EPF provide multiple benefits to plants by solubilizing minerals and facilitating nutrient acquisition.

Cited by 15 publications

References 115 publications

Exploring the Role of Debaryomyces hansenii as Biofertilizer in Iron-Deficient Environments to Enhance Plant Nutrition and Crop Production Sustainability

Exploring the Role of Debaryomyces hansenii as Biofertilizer in Iron-Deficient Environments to Enhance Plant Nutrition and Crop Production Sustainability

Metarhizium rileyi with broad‐spectrum insecticidal ability confers resistance against phytopathogens and insect pests as a phytoendophyte

Novel report on soil infection with Metarhizium rileyi against soil‐dwelling life stages of insect pests

Contact Info

Product

Resources

About