Entomopathogenic microorganisms: their role in insect pest management

Deka, Bhabesh; Baruah, Chittaranjan; Babu, Azariah

doi:10.1186/s41938-021-00466-7

Cited by 55 publications

(23 citation statements)

References 41 publications

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“…Biological control has been considered as an effective, persistent, eco-friendly option for pathogen/pest control. In particular, in the field of pest control, potential candidates such as bacteria, fungi, viruses, or endosymbionts were evaluated for their ability and suitability as biological agents [ 16 , 20 , 50 , 51 , 52 ]. Using insect-specific viruses (ISVs) as biological control agents has become a valuable method to control vector-borne diseases [ 21 , 36 , 53 , 54 , 55 , 56 ].…”

Section: Discussionmentioning

confidence: 99%

Identifying the Gut Virome of Diaphorina citri from Florida Groves

Lin

Batuman

Levy

2023

Insects

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Asian citrus psyllid (Diaphorina citri) transmits the bacterial pathogen Candidatus Liberibacter asiaticus (CLas), the putative causative agent of citrus Huanglongbing disease (HLB). Insect-specific viruses can act against insects as their natural enemies, and recently, several D. citri-associated viruses were discovered. The insect gut plays an important role as not only a pool for diverse microbes but also as a physical barrier to prevent the spread of pathogens such as CLas. However, there is little evidence of the presence of D. citri-associated viruses in the gut and of the interaction between them and CLas. Here, we dissected psyllid guts collected from five growing regions in Florida, and the gut virome was analyzed by high throughput sequencing. Four insect viruses, including D. citri-associated C virus (DcACV), D. citri densovirus (DcDV), D. citri reovirus (DcRV), and D. citri flavi-like virus (DcFLV), were identified, and their presence in the gut, including an additional D. citri cimodo-like virus (DcCLV), were confirmed with PCR-based assays. Microscopic analysis showed that DcFLV infection leads to morphological abnormalities in the nuclear structure in the infected psyllid gut cells. The complex and diverse composition of microbiota in the psyllid gut suggests a possible interaction and dynamics between CLas and the D. citri-associated viruses. Our study identified various D. citri-associated viruses that localized in the psyllid gut and provided more information that helps to evaluate the potential vectors for manipulating CLas in the psyllid gut.

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Section: Discussionmentioning

confidence: 99%

Identifying the Gut Virome of Diaphorina citri from Florida Groves

Lin

Batuman

Levy

2023

Insects

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“…However, only a few genera have been applied as entomopathogens, including entomopathogenic fungi (Beauveria, Metarhizium, Isaria, Lecanicillium and Hirsutella), entomopathogenic bacteria (Bacillus thuringiensis, B. sphaericus, B. cereus and B. popilliae), entomopathogenic virus (Nucleopolyhedrovirus/NPV and Granulovirus/GV), nematodes (Heterorhabditis, Steinernema). Some of these entomopathogens are available in commercial formulation as bioinsecticides [57].…”

Section: Utilization Of Biological Control Agentsmentioning

confidence: 99%

Crop Protection and Environment: Sustainable Control Measures of Pests and Diseases

Brotodjojo

Lakatos

2022

IOP Conf. Ser.: Earth Environ. Sci.

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The second Sustainable Development Goals determines to achieve zero starvation, accomplish food security and attain better-quality nutrition and encourage eco-friendly agriculture. The sustainability can be achieved by maintaining environments, improving capability for adjustment to climate change, unpredictable weather, and other natural catastrophes and enhancing land and soil quality progressively. The challenge in keeping high yield crops is pests and diseases attack. Control measures to suppress pests and diseases should also keep the ecosystems in balance. Pesticides that heavily used for plant protection besides their effectiveness in controlling pests and diseases also possess risks to non-target organisms. To diminish the harmful effect of pesticides, innovative methods to cut the amount of active ingredients applied have established, including developing new formulation of existing compounds, enhancing the bioactivity of active ingredients. In addition, there are also new development in plant protection products derived from natural ingredients, new mode of action and biochemical targets. Various cultural methods are considered eco-friendly measures, including habitat and cropping management, sanitation and fallowing. Furthermore, application of biocontrol agents and cultivation of resistant/tolerant crop varieties should be incorporate into plant protection as part of sustainable control methods of pests and diseases.

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“…Entomopathogenic fungi are pathogenic micro-organisms that can infect and penetrate the host and cause disease, which ultimately leads to the death of insects [9]. In the theory of integrated pest management (IPM), entomopathogenic fungi have become one of the most critical tools in the population control of agricultural pests [10][11][12]. Under natural conditions, entomopathogenic fungi mainly adhere to the surface of the host through spores.…”

Section: Introductionmentioning

confidence: 99%

First Record of Aspergillus fijiensis as an Entomopathogenic Fungus against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)

Yan

Líu

Idrees

et al. 2022

JoF

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The Asian citrus psyllid Diaphorina citri Kuwayama (Hemiptera: Liviidae) is the most widespread and devastating pest species in citrus orchards and is the natural vector of the phloem-limited bacterium that causes Huanglongbing (HLB) disease. Thus, reducing the population of D. citri is an important means to prevent the spread of HLB disease. Due to the long-term use of chemical control, biological control has become the most promising strategy. In this study, a novel highly pathogenic fungal strain was isolated from naturally infected cadavers of adult D. citri. The species was identified as Aspergillus fijiensis using morphological identification and phylogenetic analysis and assigned the strain name GDIZM-1. Tests to detect aflatoxin B1 demonstrated that A. fijiensis GDIZM-1 is a non-aflatoxin B1 producer. The pathogenicity of the strain against D. citri was determined under laboratory and greenhouse conditions. The results of the laboratory study indicated that nymphs from the 1st to 5th instar and adults of D. citri were infected by A. fijiensis GDIZM-1. The mortality of nymphs and adults of D. citri caused by infection with A. fijiensis increased with the concentration of the conidial suspension and exposure time, and the median lethal concentration (LC50) and median lethal time (LT50) values gradually decreased. The mortality of D. citri for all instars was higher than 70%, with high pathogenicity at the 7th day post treatment with 1×108 conidia/mL. The results of the greenhouse pathogenicity tests showed that the survival of D. citri adults was 3.33% on the 14th day post-treatment with 1 × 108 conidia/mL, which was significantly lower than that after treatment with the Metarhizium anisopliae GDIZMMa-3 strain and sterile water. The results of the present study revealed that the isolate of A. fijiensis GDIZM-1 was effective against D. citri and it provides a basis for the development of a new microbial pesticide against D. citri after validation of these results in the field.

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Entomopathogenic microorganisms: their role in insect pest management

Cited by 55 publications

References 41 publications

Identifying the Gut Virome of Diaphorina citri from Florida Groves

Identifying the Gut Virome of Diaphorina citri from Florida Groves

Crop Protection and Environment: Sustainable Control Measures of Pests and Diseases

First Record of Aspergillus fijiensis as an Entomopathogenic Fungus against Asian Citrus Psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae)

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