Entomotoxic properties of Dioclea violacea lectin and its effects on digestive enzymes of Anagasta kuehniella (Lepidoptera)

Oliveira, Carolina Turatti; Kunz, Daniele; Silva, Carlos P.; Macedo, Maria Lígia Rodrigues

doi:10.1016/j.jinsphys.2015.07.007

Cited by 14 publications

(11 citation statements)

References 48 publications

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“…Reduction in the weight and efficiency of food conversion into body mass were detected in Anagasta kuehniella larvae, when these insects were maintained with an artificial diet containing M. oleifera seed lectins [30,31]. The ingestion of Dioclea violacea lectin also decreased A. kuehniella larval mass without affecting its survival [32].…”

Section: Discussionmentioning

confidence: 96%

“…The D. violacea lectin, which was not able to kill A. kuehniella larvae, but affected their nutrition, was able to bind to the peritrophic membrane [32]. The chitin-binding lectins from the bark, heartwood, and leaf of M. urundeuva were able to induce apoptosis in the digestive and enteroendocrine cells of N. corniger gut and consequently block the absorption of nutrients and deregulate the coordination of enzyme release into the gut lumen [39].…”

Section: Discussionmentioning

confidence: 99%

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Effects of Opuntia ficus-indica lectin on feeding, survival, and gut enzymes of maize weevil, Sitophilus zeamais

et al. 2018

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In this study, the effects of Opuntia ficus-indica lectin (OfiL) on the survival and nutritional parameters of Sitophilus zeamais (maize weevil) adults were evaluated. OfiL was incorporated into the artificial diets at concentrations of 15, 60, and 95 mg/g (mg of lectin per g of wheat flour). Mortality was evaluated after 7 and 15 days, and the amount of food ingested and the weight of the insects were determined on the 7th day. In addition, the in vitro effects of OfiL on the gut enzymes of the insect were investigated. The ingestion of OfiL did not show any significant difference in the mortality rates compared to control. The relative consumption rate was also similar to that of the control, and no deterrent effect was detected. However, the values of the relative biomass variation and the efficiency of ingested food conversion were negative in the treatments at 60 and 95 mg/g, showing that lectin ingestion resulted in weight loss. OfiL exhibited a stimulatory effect on the protease activity from S. zeamais gut extract, which may cause uncontrolled hydrolysis of proteins in the digestive tract. This lectin did not promote significant alteration in the amylase activity. In conclusion, OfiL was able to exert anti-nutritional effects without causing a deterrent effect.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Effects of Opuntia ficus-indica lectin on feeding, survival, and gut enzymes of maize weevil, Sitophilus zeamais

et al. 2018

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“…Its insecticidal activity is due to the affinity of plant lectins to carbohydrate structures present in the midgut of insects (Bolognesi et al, 2008), affecting the functioning of their physiological processes. Oliveira et al (2015) investigated the effects of Dioclea violacea lectin (DVL) on the larval development of Anagasta kuehniella and observed that chronic exposure to DVL affected larval development without affecting survival. Its entomotoxic activity was facilitated by several factors, such as resistance of the lectin to proteolytic digestion, binding to chitin molecules present in the peritrophic membrane of the midgut of larvae and an effect on the activity of digestive enzymes.…”

Section: Miscellaneousmentioning

confidence: 99%

Research Article A proteomic approach to guarana seed and pericarp maturation.

Souza¹,

Lopez-Losano

Angelo³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Paullinia cupana Kunth var. sorbilis (Mart.) Ducke, the cultivated guarana plant, is native to the Amazon and has been valued for its medicinal, stimulant and energetic properties for centuries. The seeds are the main commercial product of the plant and the source of high amounts of purine alkaloids (caffeine and theobromine) and polyphenols (flavonoids, catechins, and tannins). Proteins involved in the development and maturation of guarana fruits in its native habitat are interesting issues for proteomics. This study presents the proteomic profile of the seed and pericarp of healthy guarana in different maturation stages. Protein contents were higher in the mature seed compared to other stages due to the accumulation of storage proteins -11S globulins. Proteins selected for identification by mass spectrometry are mostly related to stress responses and defense and this is not unexpected for fast growing and differentiating reproductive tissues.

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“…Lectin‐FITC conjugates have been used to identify glycan moieties in biomolecules, components of the cytoskeleton, and receptors on human endothelial cells . Conjugation with FITC revealed that the Dioclea violacea lectin binds to the peritrophic matrix of Anagasta kuehniella larvae and that the Rhizoctonia solani lectin penetrates the peritrophic matrix of Tribolium castaneum …”

Section: Introductionmentioning

confidence: 99%

Ovicidal lectins from Moringa oleifera and Myracrodruon urundeuva cause alterations in chorionic surface and penetrate the embryos of Aedes aegypti eggs

Alves

Soares

Bento

et al. 2019

Pest Management Science

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BACKGROUND: Lectins from Moringa oleifera seeds (WSMoL), Myracrodruon urundeuva bark (MuBL), and heartwood (MuHL) are larvicidal agents against Aedes aegypti; in addition, WSMoL is an ovicidal agent against this mosquito. In this work, we evaluated the ovicidal activity of MuBL and MuHL by determining the concentrations that reduce the hatching rates by 50% in 72 h (EC 50 ). The effects of WSMoL, MuBL, and MuHL on the ultrastructure of the eggs' surface were assessed by scanning electron microscopy (SEM). In addition, the ability of these lectins to penetrate the eggs was investigated by using conjugates of the lectins with fluorescein isothiocyanate (FITC). RESULTS: MuBL andMuHL were ovicidal agents with EC 50 of 0.26 and 0.80 mg/mL (260 and 800 ppm), respectively. SEM images of eggs treated with WSMoL for 24 h revealed discontinuity of the exochorionic network and the absence of the exochorionic cells and their tubercles. After 48 and 72 h of incubation, strong deformation and degeneration of egg surfaces were observed. In MuBL and MuHL-treated eggs, the presence of lumps on the surface of the eggs, disappearance of the exochorionic network and the decrease and deformation of tubercles were observed. Lastly, fluorescence microscopy revealed that the three lectins were able to enter the eggs and reach the digestive tract of the embryos. CONCLUSION: WSMoL, MuBL, and MuHL are ovicidal agents on A. aegypti that have differing efficiencies in terms of how they cause alterations in the chorionic surface and in terms of their ability to penetrate the eggs.

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Entomotoxic properties of Dioclea violacea lectin and its effects on digestive enzymes of Anagasta kuehniella (Lepidoptera)

Cited by 14 publications

References 48 publications

Effects of Opuntia ficus-indica lectin on feeding, survival, and gut enzymes of maize weevil, Sitophilus zeamais

Effects of Opuntia ficus-indica lectin on feeding, survival, and gut enzymes of maize weevil, Sitophilus zeamais

Research Article A proteomic approach to guarana seed and pericarp maturation.

Ovicidal lectins from Moringa oleifera and Myracrodruon urundeuva cause alterations in chorionic surface and penetrate the embryos of Aedes aegypti eggs

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