Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests

Tomson, Majesh; Sahayaraj, K.; Sayed, Samy; El-Arnaouty, S. A.; Petchidurai, Ganeshan

doi:10.1016/j.jksus.2021.101595

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…Later studies explored that the induced systemic resistance in tobacco and tomato plants is manifested by triggering of plant SA related defense pathways by a F8‐polysaccharide 36,37 . Interestingly, elicitor protein PeBb1 used in this study has been demonstrated to upregulate the expression levels of SA, JA and ET related plant defense genes in different plants against various sucking and chewing insect pests 17,20,28,38 . Djonović et al 39 .…”

Section: Discussionmentioning

confidence: 80%

“…36,37 Interestingly, elicitor protein PeBb1 used in this study has been demonstrated to upregulate the expression levels of SA, JA and ET related plant defense genes in different plants against various sucking and chewing insect pests. 17,20,28,38 Djonović et al 39 showed that a small elicitor protein (designated as Sm1) derived from a biocontrol fungus Trichoderma virens induced systemic resistance in cotton plants against foliar infection by Colletotrichum sp. This study showed triggering of the production of reactive oxygen species and plant defense related gene expressions both locally and systemically within cotton plant tissues.…”

Section: Discussionmentioning

confidence: 99%

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Systemic resistance induced in tomato plants by Beauveria bassiana‐derived proteins against tomato yellow leaf curl virus and aphid Myzus persicae

Basit,

Humza,

Majeed

et al. 2024

Pest Management Science

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BACKGROUNDTomato (Solanum lycopersicum L.) is an economically important vegetable crop around the globe. Tomato yellow leaf curling (TYLC) is the most devastating viral disease posing a serious threat to tomato production throughout the tropical and subtropical world. Induction of microbe‐mediated systemic resistance in plants have been of great interest in recent years as a novel microbiological tool in disease and insect pest management. This in‐vitro study aimed to determine the effectiveness of different strains (BB252, BB72 and ARSEF‐2860) of a hypocreal fungus Beauveria bassiana against TYLCV disease and aphid Myzus persicae. Potted tomato plants exogenously treated with conidial and filtrate suspensions of B. bassiana strains and of their partially purified or purified proteins were exposed to TYLCV inoculum and aphid M. persicae.RESULTSResults showed a significant suppression of TYLCV disease severity index by the exogenous application of conidial, filtrate and protein treatments of all B. bassiana strains and this response was directly proportional to the treatment concentration. Similarly, mean fecundity rate of M. persicae was also significantly reduced by the highest concentration of ARSEF‐2860‐derived elicitor protein PeBb1, followed by the highest concentrations of BB252‐ and BB72‐derived partially purified proteins. Moreover, these B. bassiana‐derived proteins also caused a significant upregulation of most of the plant immune marker genes associated with plant defense.CONCLUSIONOverall study findings suggest that these above mentioned B. bassiana strains and their partially purified or purified elicitor proteins could be effective biological tools for the management of TYLCV and aphid infestation on tomato plants.This article is protected by copyright. All rights reserved.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Systemic resistance induced in tomato plants by Beauveria bassiana‐derived proteins against tomato yellow leaf curl virus and aphid Myzus persicae

Basit,

Humza,

Majeed

et al. 2024

Pest Management Science

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“…Apart from their efficacy, several EPFs produced a variety of metabolically active toxin compounds that impede the defense mechanisms of the host ( Zhang & Xia, 2009 ; Gibson et al, 2014 ). Insecticidal activity was previously reported for M. anisopliae and B. bassiana crude extracts and their purified entomotoxic fractions when given or injected to several insect pests ( Sahayaraj & Tomson, 2010 ; Freed et al, 2012 ; Keerio et al, 2020 , Tomson et al, 2021 ). The current study exhibited the sub-lethal effect of crude soluble toxic protein (CSE) extracted from transformed B. bassiana against P. solenopsis and A. craccivora and its effect on the concentration of hemolymphic Na + and K + levels in them.…”

Section: Discussionmentioning

confidence: 86%

“…Similarly, earlier findings have revealed the strong sub-lethal and toxic effects of crude toxins isolated from M. anisopliae and B. bassiana (un-transformed) on phloem feeders such as Dysdercus cingulatus (Fabricius) ( Sahayaraj & Tomson, 2010 ), A. gossypii ( Gurulingappa, McGee & Sword, 2011 ), Plutella xylostella (L.) ( Freed et al, 2012 ), Bemisia tabaci (Gennadius) ( Keerio et al, 2020 ). Likewise, Tomson et al (2021) reported 61.7% mortality in P. solenopsis by the application of fractionized fungal filtrate of B. bassiana . After 10 days of treatment, the transformed B. bassiana fungal strain (BbLqqIT1-3) could cause only 39% mortality in P. solenopsis at 1 * 10 9 spore/ml; and the survived 61% nymphs could live for further 14 days (data not presented).…”

Section: Discussionmentioning

confidence: 94%

Bioefficacy of engineered Beauveria bassiana with scorpion neurotoxin, LqqIT1 against cotton mealybug, Phenacoccus solenopsis and cowpea aphid, Aphis craccivora

Rajput,

Suroshe,

Yadav

et al. 2023

PeerJ

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Cotton mealybug, Phenacoccus solenopsis (Tinsley) and cowpea aphid Aphis craccivora (Koch) are notorious polyphagous, hemipteran sap sucking insect pests. A recombinant toxin gene ‘LqqIT1’ from the scorpion Leiurus quinquestriatus quinquestriatus (Ehrenberg) was cloned in the pAL1 fungal expression vector and then expressed in the entomopathogenic fungus Beauveria bassiana (Balasmo) using genetic modification techniques. The genetically transformed B. bassiana strain (BbLqqIT1-3) and its un-transformed parent strain (Bb-C) were screened to infect the third instar nymphs of P. solenopsis and first instar nymph of A. craccivora through leaf treatment and topical application (spray) method at 1 * 107 spores per ml concentration. The recombinant strain BbLqqIT1-3 was highly pathogenic against A. craccivora but non pathogenic to P. solenopsis. BbLqqIT1-3 induced 72 and 43.33% mortality in A. craccivora nymphs 96 h after leaf treatment and topical application, respectively. The nymphs of A. craccivora infected with BbLqqIT1-3 displayed classical neurotoxic symptoms such as sluggishness, solublize and liquification of the body. Crude soluble toxin protein, BbLqqIT1a-CSE and Bb-WT-CSE was extracted from the BbLqqIT1-3 and Bb-C, respectively using ammonium sulphate precipitation method, and their oral toxicity was analyzed at 5 µg/ml concentration. The survival of the studied insects was negatively affected by the crude soluble toxin extracts. The LT50 values of BbLqqIT1a-CSE against P. solenopsis and A. craccivora were 22.18 and 17.69 h, respectively. Exposure to crude soluble toxin extracts also accounted for the imbalance of ionic concentrations in the hemolymph of treated insects such as hyperpotassemia (3.53–8.18 meq/ml) in the P. solenopsis and hypopotassemia (7.52–0.47 meq/ml) in A. craccivora. The transformed fungus BbLqqIT1-3 strain exhibited promising results in invitro study.

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“…Beauveria bassiana is one of the most widely studied and applied entomopathogenic fungi in the field of biological control of pests, with more than 360 species. It has the advantages of high toxicity, wide application, and no environmental pollution (Mascarin and Jaronski, 2016;Liu et al, 2021;Tomson et al, 2021). It has been widely used to control agricultural pests such as Bactrocera dorsalis (Hendel), Tetranychus urticae (Koch), and Bemisia tabaci (Gennadius) (Zibaee et al, 2013;Liu et al, 2017;Guo et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Distribution and interaction of the suitable areas of Beauveria bassiana and Bactrocera dorsalis (Hendel)

et al. 2022

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Climate is a key factor affecting the potential distribution of insects, and the host is another important constraint for the distribution of pests. To elucidate changes in the potential distribution of Beauveria bassiana under climate change scenarios, this paper used the data of two different greenhouse gas (GHG) emission scenarios (RCP2.6, RCP8.5) to predict the potential distribution of B. bassiana and its typical host, Bactrocera dorsalis (Hendel), based on the MaxEnt model. Then, the potential distribution of B. bassiana and B. dorsalis (Hendel) was compared, and their suitable growth area’s change and expansion trend under two different GHG emission scenarios were mastered. The results of this study show that the potential distribution area of B. bassiana will increase by 2,050 under the RCP8.5 climate scenario, mainly in central Europe and southwestern Asia, with an increased area of 3.28 × 105 km2. However, under the climate scenario of RCP2.6, the potential distribution area for B. bassiana decreased by 2.0 × 105 km2, mainly in North America. This study will provide a theoretical basis for the control of B. dorsalis (Hendel) with B. bassiana.

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Entomotoxic proteins of Beauveria bassiana Bals. (Vuil.) and their virulence against two cotton insect pests

Cited by 8 publications

References 25 publications

Systemic resistance induced in tomato plants by Beauveria bassiana‐derived proteins against tomato yellow leaf curl virus and aphid Myzus persicae

Systemic resistance induced in tomato plants by Beauveria bassiana‐derived proteins against tomato yellow leaf curl virus and aphid Myzus persicae

Bioefficacy of engineered Beauveria bassiana with scorpion neurotoxin, LqqIT1 against cotton mealybug, Phenacoccus solenopsis and cowpea aphid, Aphis craccivora

Distribution and interaction of the suitable areas of Beauveria bassiana and Bactrocera dorsalis (Hendel)

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