Entrapment of enzyme-linked magnetic nanoparticles within poly(ethylene glycol) hydrogel microparticles prepared by photopatterning

Park, Sami; Lee, Yeol; Kim, Dae Nyun; Park, Sangphil; Jang, Eunji; Koh, Won Gun

doi:10.1016/j.reactfunctpolym.2009.02.001

Cited by 25 publications

(16 citation statements)

References 34 publications

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“…The small size, high stability, high capacity and strong magnetic responsiveness of super-paramagnetic ferrite nanoparticles can have significant influence on adsorption and desorption of the proteins. Because of these reasons, lots of study about immobilization or adsorption of enzymes with ferrite nanoparticle-polymer composites has been under consideration in literature [8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Adsorption and desorption studies of lysozyme by Fe3O4–polymer nanocomposite via fluorescence spectroscopy

Koç

Alveroğlu

2015

Journal of Molecular Structure

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Section: Introductionmentioning

confidence: 99%

Adsorption and desorption studies of lysozyme by Fe3O4–polymer nanocomposite via fluorescence spectroscopy

Koç

Alveroğlu

2015

Journal of Molecular Structure

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“…swelling or phase transition) for making stimuli-responsive smart materials (42)(43)(44). Other signaling mechanisms such as electrochemical detection have also been demonstrated while few examples of optical sensors are known (25,30,(45)(46)(47)(48)(49). In particular, very few colorimetric sensors have been reported (25,45,50,51).…”

Section: Introductionmentioning

confidence: 99%

DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection

Baeissa

Dave

Smith

et al. 2010

ACS Appl. Mater. Interfaces

127

115

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Highly sensitive and selective DNA detection plays a central role in many fields of research and various assay platforms have been developed. Compared to homogeneous DNA detection, surface immobilized probes allow washing steps and signal amplification to give higher sensitivity. Previously research was focused on developing glass or gold based surfaces for DNA immobilization, we herein report hydrogel immobilized DNA. Specifically, acrydite-modified DNA was covalently functionalized to the polyacrylamide hydrogel during gel formation. There are several advantages of these DNAfunctionalized monolithic hydrogels. First, they can be easily handled in a way similar to that in homogeneous assays. Second, they have a low optical background where in combination with DNAfunctionalized gold nanoparticles, even ~0.1 nM target DNA can be visually detected. By using the attached gold nanoparticles to catalyze the reduction of Ag + , as low as 1 pM target DNA can be detected. The gels can be regenerated by a simple thermal treatment and the regenerated gels perform similarly compared to freshly prepared ones. The amount of gold nanoparticles adsorbed through DNA hybridization decreases with increasing gel percentage. Other parameters including DNA concentration, DNA sequence, ionic strength of the solution and temperature have also been systematically characterized in this study.

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“…In another study, Park et al showed that increasing the molecular weight of the PEGDA precursor from 575 to 3400 g mol −1 resulted in improved kinetics for glucose readout at the cost of higher probe leaching, due to an increased porosity [36]. To circumvent the leaching issue, the authors covalently captured the enzyme on magnetic nanoparticles of larger diameter (~30nm) first, that were then efficiently embedded within the hydrogel mesh.…”

Section: Selecting a Materials And A Strategy For Probe Immobilizationmentioning

confidence: 99%

“…The amount of swelling increased with the precursor molecular weight (40% for PEGDA700, 80% for PEGDA3400). However, the particle deformation was isotropic and the overall shape and aspect ratio were not affected [36, 67]. Another study found that increasing the percentage of PEGDA in the prepolymer solution increased the tendency of particles to uptake water immediately after polymerization [33].…”

Section: Selecting a Materials And A Strategy For Probe Immobilizationmentioning

confidence: 99%

Hydrogel microparticles for biosensing

Goff

Srinivas

Hill³

et al. 2015

European Polymer Journal

180

144

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Due to their hydrophilic, biocompatible, and highly tunable nature, hydrogel materials have attracted strong interest in the recent years for numerous biotechnological applications. In particular, their solution-like environment and non-fouling nature in complex biological samples render hydrogels as ideal substrates for biosensing applications. Hydrogel coatings, and later, gel dot surface microarrays, were successfully used in sensitive nucleic acid assays and immunoassays. More recently, new microfabrication techniques for synthesizing encoded particles from hydrogel materials have enabled the development of hydrogel-based suspension arrays. Lithography processes and droplet-based microfluidic techniques enable generation of libraries of particles with unique spectral or graphical codes, for multiplexed sensing in biological samples. In this review, we discuss the key questions arising when designing hydrogel particles dedicated to biosensing. How can the hydrogel material be engineered in order to tune its properties and immobilize bioprobes inside? What are the strategies to fabricate and encode gel particles, and how can particles be processed and decoded after the assay? Finally, we review the bioassays reported so far in the literature that have used hydrogel particle arrays and give an outlook of further developments of the field.

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Entrapment of enzyme-linked magnetic nanoparticles within poly(ethylene glycol) hydrogel microparticles prepared by photopatterning

Cited by 25 publications

References 34 publications

Adsorption and desorption studies of lysozyme by Fe3O4–polymer nanocomposite via fluorescence spectroscopy

Adsorption and desorption studies of lysozyme by Fe3O4–polymer nanocomposite via fluorescence spectroscopy

DNA-Functionalized Monolithic Hydrogels and Gold Nanoparticles for Colorimetric DNA Detection

Hydrogel microparticles for biosensing

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