Entrapment of viable microorganisms by SiO2 sol-gel layers on glass surfaces: Trapping, catalytic performance and immobilization durability of Saccharomyces cerevisiae

Inama, L.; Dirè, Sandra; Carturan, G.; Cavazza, A.

doi:10.1016/0168-1656(93)90113-2

Cited by 117 publications

(61 citation statements)

References 18 publications

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“…Haematological condition such as total erythrocyte count (RBC), white blood cells (WBC) count, haemoglobin count (Hb) and total protein in the serum of fish is an effective tool that can be used to evaluate physiological and pathological changes in fish (Inama et al, 1993). Moreover, WBC has been regularly used as indicators of health status in fish because WBC is key components of innate immune defense and involved in regulation of immunological function in fish including ornamental fish (Betta sp) (Zhou et al, 2010;Ekman et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured

et al. 2016

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This research aimed to determine the phytochemicals content of Terminalia catappa leaves extract (TCL) and its effects on the survival and blood profiles of ornamental fish (Betta sp) Ninety fish were randomly assigned into six triplicates groups and reared in various concentration of TCL: 0 (control), 125, 250, 375, 500, 625 ppm for 30 days. Temperature, Dissolve oxygen (DO), and pH were monitored during the trial. After 30 days, survival, Red Blood Cells (RBC), White Blood Cells (WBC), haemoglobin (Hb), lymphocyte, and total protein serum (TPS) were analyzed. Based on the phytochemicals test, saponin, triterpenoid, quinon, phenolic, tannin, and flavonoid were detected on the TCL. Temperature and DO were not affected by any concentration of TCL. The lowest pH (5.05) was found in fish medium immersed with 625 ppm of TCL. Adding TCL above 375 ppm resulted in significantly higher survival, RBC, and Hb. The highest WBC was found in fish immersed with 625 ppm whereas the lowest lymphocyte was found in fish immersed with 375 of TCL. However, immersing any various concentration of TCL did not affect on the TPS. In summary, immersing TCL above 375 ppm is beneficial to enhance survival, RBC, WBC, and Hb of Betta sp.

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Section: Introductionmentioning

confidence: 99%

The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured

et al. 2016

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“…Sol-gel technology has also been used for encapsulating proteins, cells, microbe and immunoreagents. [19][20][21] It is interesting to investigate the possibility of preparing an electrochemical immunosensor for Brucella antibody determination based on the silica sol-gel A novel amperometric immunosensor setup is described which uses horseradish peroxidase (HRP) as a label in conjunction with a current-based Brucella sensor. The Bacteria modified immunosensor was constructed by using a biocomposite formed by dispersing graphite powder into a mixture of Brucella melitensis and silicate polymer gel.…”

Section: Introductionmentioning

confidence: 99%

Bacteria-modified Amperometric Immunosensor for a Brucella melitensis Antibody Assay

Gong

Shen

et al. 2002

ANAL. SCI.

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Brucellosis is an infectious disease that spreads easily between animals and even human beings. There is a critical fact that once a flock of livestocks is infected by Brucella, the prevalent brucellosis would cause over half of the infected pregnant ones to abort. 1 A sensitive and simple detection of Brucella antibody is essential in clinic and veterinary diagnosis. Some traditional diagnostic procedures, such as bacterioscopy (BS), agglutination test (AT), complement fixation test (CFT), precipitation reaction test (PRT), SPA-co-agglutination test (SCAT), enzyme-linked immunosorbent assay (ELISA), radio immunoassay (RIA) and indirect fluorescent antibody (IFAT) test, have been employed in clinical analysis. 2 However, some of these approaches are rather time-consuming or requiring highly qualified personnel; others depend on sophisticated instrumentation. Moreover, most of these procedures are only adapted for the qualitative or semiquantitative detection for Brucella assays. To obtain satisfactory results, most of the diagnostic procedures have to be coupled with another independent test; otherwise, a part of the infected samples may escape the screening test. Developing new, simple and more sensitive quantitative diagnosis methods has attracted the considerable interest.Electrochemical immunosensors are robust tools of immunoanalytical means that have been the subject of continued research and development in recent years. These methods generally involve the production or use of an electrochemical active substance for signal generation.Immunoenzymatic sensors involving the enzyme label and the specificity of an antigen-antibody binding have an effect of chemical amplification and relatively high selectivity. The advantages of electrochemical immunosensors include speed, accuracy, selectivity and sensitivity. paraffin, 6 nafion, 7 chitosan, 8 were used as a matrix material to prepare immunosensors. They are mostly organic polymers, which tend to swell when dipped into a solvent, leading to a shift of the response signal. The activity of biomolecules in these materials used for immobilization decreases appreciably and the immobilized biomolecules do not retain their activity over a long time period. Recently, in the so-called sol-gel process, some biomaterials were trapped into a silicate glass matrix under mild conditions. [10][11][12] Biomolecules show considerable retention of their activity in such immobilization matrices. The other outstanding feature of such a process is that small substrate molecules can diffuse into the pores of the polymeric silica matrix and reach the site of the bioreagent. The Sol-Gel process for biomolecules immobilization seems to be very promising, especially due to the mild conditions that are used for the immobilization process as well as the simplicity of the procedure involved. 13In addition, the immobilization material can be optimized so that transparent monoliths, thin films or fine particles, are formed depending on the ultimate application of the preparation. 14-17 Brau...

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“…The immobilized biocatalyst survives experimental processing and can be successfully applied e.g. for the degradation of sucrose [73].…”

Section: ____________________________________________________________mentioning

confidence: 99%

Bioactive Sol-Gel Coatings

Böttcher¹

2000

J. prakt. Chem.

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Entrapment of viable microorganisms by SiO2 sol-gel layers on glass surfaces: Trapping, catalytic performance and immobilization durability of Saccharomyces cerevisiae

Cited by 117 publications

References 18 publications

The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured

The Effects of Terminalia catappa L. Leaves Extract on the Water Quality Properties, Survival and Blood Profile of Ornamental fish (Betta sp) Cultured

Bacteria-modified Amperometric Immunosensor for a Brucella melitensis Antibody Assay

Bioactive Sol-Gel Coatings

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