Entrapping quercetin in silica/polyethylene glycol hybrid materials: Chemical characterization and biocompatibility

Barrino

Poggetto³

et al. 2020

Materials

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The sol–gel route represents a valuable technique to obtain functional materials, in which organic and inorganic members are closely connected. Herein, four hybrid materials, containing caffeic acid entrapped in a silica matrix at 5, 10, 15, and 20 wt.%, were synthesized and characterized through Fourier-Transform Infrared (FT-IR) and Ultraviolet-Visible (UV–Vis) spectroscopy. FT-IR analysis was also performed to evaluate the ability to induce the hydroxyapatite nucleation. Despite some structural changes occurring on the phenol molecular skeleton, hybrid materials showed scavenging properties vs. 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2′-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid) radical cation (ABTS•+), which was dependent on the tested dose and on the caffeic acid wt.%. The SiO2/caffeic acid materials are proposed as valuable antibacterial agents against Escherichia coli and Enterococcus faecalis.

Section: Introductionmentioning

confidence: 99%

New SiO2/Caffeic Acid Hybrid Materials: Synthesis, Spectroscopic Characterization, and Bioactivity

Barrino

Poggetto³

et al. 2020

Materials

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“…After 30 min, the absorption at 515 nm was measured in reference to a blank by a Shimadzu UV-1700 spectrophotometer. The results were expressed in terms of the percentage decrease of the initial DPPH • radical absorption by the test samples [ 14 , 15 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

“…The determination of ABTS •+ solution scavenging capacity was estimated as previously reported [ 14 , 15 , 16 ], with slight modifications. The ABTS radical cation was generated by reacting ABTS (2,2′-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid); 7.0 mM) and potassium persulfate (2.45 mM).…”

Section: Methodsmentioning

confidence: 99%

“…The sol–gel technique was employed for the preparation of carbon composite electrode modified with electroless deposition of chlorogenic acid, which were evaluated for their stability and electrochemical properties was [ 10 ]. The high versatility of sol–gel routes for the formulation of organic-inorganic hybrid materials [ 11 , 12 , 13 ], together with data from our recent researches aimed at entrapping another natural antioxidant compound, such as quercetin, in a silica matrix [ 14 , 15 , 16 ], intrigued us to investigate the possibility of synthesizing new materials having chlorogenic acid as the organic component. Thus, silica-based materials, differing in their CGA amount (5 wt%, 10 wt%, 15 wt%, and 20 wt%), were synthetized and characterized by Fourier transform infrared spectroscopy (FTIR) and UV-VIS spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

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Synthesis of Bioactive Chlorogenic Acid-Silica Hybrid Materials via the Sol–Gel Route and Evaluation of Their Biocompatibility

Pacifico

2017

Materials

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Natural phenol compounds are gaining a great deal of attention because of their potential use as prophylactic and therapeutic agents in many diseases, as well as in applied science for their preventing role in oxidation deterioration. With the aim to synthetize new phenol-based materials, the sol–gel method was used to embed different content of the phenolic antioxidant chlorogenic acid (CGA) within silica matrices to obtain organic-inorganic hybrid materials. Fourier transform infrared (FTIR) measurements were used to characterize the prepared materials. The new materials were screened for their bioactivity and antioxidant potential. To this latter purpose, direct DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid) methods were applied: radical scavenging capability appeared strongly dependent on the phenol amount in investigated hybrids, and became pronounced, mainly toward the ABTS radical cation, when materials with CGA content equal to 15 wt% and 20 wt% were analyzed. The in vitro biocompatibility of the synthetized materials was estimated by using the MTT assay towards fibroblast NIH 3T3 cells, human keratinocyte HaCaT cells, and the neuroblastoma SH-SY5Y cell line. As cell viability and morphology of tested cell lines seemed to be unaffected by new materials, the attenuated total reflectance (ATR)-FTIR method was applied to deeply measure the effects of the hybrids in the three different cell lines.

“…The sol-gel route represented a consolidated technique in our laboratories, effectively applied with the aim to obtain new biocompatible and bioactive organic-inorganic hybrid materials, in which different natural compounds were embedded in a silica matrix, with or without the addition of polymers (e.g. polyethylene glycol, poly(ε-caprolactone)) [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

New SiO<sub>2</sub>/Caffeic Acid Hybrid Materials: Synthesis, Spectroscopic Characterization and Bioactivity

Barrino

Poggetto³

et al. 2019

Preprint

Self Cite

Sol-gel route represents a valuable technique to obtain functional materials, in which organic and inorganic members are closely connected. Herein four hybrid materials, containing caffeic acid entrapped in a silica matrix at 5, 10, 15 and 20 wt%, were synthesized and characterized through FT-IR and UV-Vis spectroscopy. FT-IR analysis was also performed to evaluate the ability to induce the hydroxyapatite nucleation. Despite some structural changes occurred on the phenol molecular skeleton, hybrid materials showed scavenging properties vs DPPH and ABTS + radicals, which was dependent on the tested dose and on the caffeic acid wt%. Finally, the SiO2/caffeic acid materials have been proposed as valuable antibacterial agents against Escherichia coli and Enterococcus faecalis.